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Locus table
  Locus Name D2Mit253
Chromosome 2
MGI Position (cM) 50.3
Position (cM) 52.24
Ensembl Chromosome 2
Position (bp)(Length)(Chr.)(OLD) 92391796-92391936 (141) (Chr.2)
Position (GRCm38) 92590957-92591097 (141) (Chr.2)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism JF1>MSM>B6=SM=SWR>129>PGN2>A/J

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image

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National Institute of Genetics