 |
 |
| |
Locus Name |
DXMit124 |
| Chromosome |
X
|
| MGI Position (cM) |
|
| Position (cM) |
2.25
|
| Ensembl Chromosome |
X
|
| Position (bp)(Length)(Chr.)(OLD) |
8492715-8492893 (179) (Chr.X), 8673940-8674108 (169) (Chr.X)
|
| Position (GRCm38) |
9335756-9335934 (169) (Chr.X), 9516981-9517149 (169) (Chr.X)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
BLG2>MSM>JF1>B6=PGN2>SWN>A/J>SWR=NC/Nga=NC>129=SM>SK/Cam=NC/jic=DBA>CBA>NJL>CAST |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC/Nga
|
NC/jic
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
| 178 |
172 |
174 |
172 |
175 |
166 |
168 |
174 |
168 |
174 |
178 |
168 |
164 |
193 |
177 |
187 |
181 |
158 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
|
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