|
|
|
Locus Name |
D8Mit298 |
Chromosome |
8
|
MGI Position (cM) |
29.0
|
Position (cM) |
31.61
|
Ensembl Chromosome |
8
|
Position (bp)(Length)(Chr.)(OLD) |
49785096-49785218 (123) (Chr.8)
|
Position (GRCm38) |
48286279-48286401 (123) (Chr.8)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
JF1>MSM>B6=A/J>SWR=SM=PGN2=129 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
|
|
|