|
|
|
Locus Name |
D11Mit155 |
Chromosome |
11
|
MGI Position (cM) |
|
Position (cM) |
29.69
|
Ensembl Chromosome |
11
|
Position (bp)(Length)(Chr.)(OLD) |
58644635-58644749 (115) (Chr.11), 58654663-58654773 (111) (Chr.11)
|
Position (GRCm38) |
58828440-58828554 (112) (Chr.11), 58838468-58838578 (112) (Chr.11)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
MSM>A/J=SWR>JF1=B6=PGN2>129=NC/Nga>SM=NC/jic |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
NC/jic
|
NC/Nga
|
PGN2
|
MSM
|
JF1
|
113 |
102 |
121 |
104 |
121 |
102 |
104 |
113 |
123 |
113 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
|
|
|