 |
 |
| |
Locus Name |
D12Mit126 |
| Chromosome |
12
|
| MGI Position (cM) |
22.0
|
| Position (cM) |
19.43
|
| Ensembl Chromosome |
12
|
| Position (bp)(Length)(Chr.)(OLD) |
53950206-53950341 (136) (Chr.12)
|
| Position (GRCm38) |
53028586-53028721 (136) (Chr.12)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
BLG2>MSM>129>CAST>SWN>SK/Cam>SWR>CBA=A/J>NJL>DBA=NC=B6>SM>PGN2>JF1 |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
| 137 |
135 |
141 |
150 |
139 |
139 |
137 |
137 |
134 |
144 |
138 |
186 |
145 |
153 |
121 |
149 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
|
|
|
|
|
