 |
 |
| |
Locus Name |
D8Mit156 |
| Chromosome |
8
|
| MGI Position (cM) |
73.0
|
| Position (cM) |
73.7
|
| Ensembl Chromosome |
8
|
| Position (bp)(Length)(Chr.)(OLD) |
131781594-131781732 (139) (Chr.8)
|
| Position (GRCm38) |
128895504-128895642 (139) (Chr.8)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
BLG2>NJL>SWN=CAST>SK/Cam=CBA>DBA>A/J=SWR=SM=B6>NC>PGN2>129=MSM=JF1 |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
| 147 |
147 |
147 |
95 |
147 |
155 |
154 |
138 |
135 |
155 |
165 |
177 |
159 |
95 |
95 |
159 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
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