|
|
|
Locus Name |
D14Mit37 |
Chromosome |
14
|
MGI Position (cM) |
27.5
|
Position (cM) |
29.05
|
Ensembl Chromosome |
14
|
Position (bp)(Length)(Chr.)(OLD) |
61337553-61337691 (139) (Chr.14)
|
Position (GRCm38) |
62383443-62383581 (139) (Chr.14)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
NC/Nga>NC/jic=B6>129>PGN2>SWR=SM=A/J>MSM>JF1 |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
NC/Nga
|
NC/jic
|
PGN2
|
MSM
|
JF1
|
136 |
91 |
91 |
134 |
91 |
144 |
136 |
93 |
62 |
61 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
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