![](/mouse/mmdbj/images/transparent.gif;jsessionid=D12AACAEB9655C3E0C42462624DE7E4E) |
![](/mouse/mmdbj/images/titleMicrosatelliteEn.gif;jsessionid=D12AACAEB9655C3E0C42462624DE7E4E) |
|
Locus Name |
D8Mit204 |
Chromosome |
0
|
MGI Position (cM) |
20.0
|
Position (cM) |
22.69
|
Ensembl Chromosome |
|
Position (bp)(Length)(Chr.)(OLD) |
|
Position (GRCm38) |
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
A/J>PGN2=SM=129=SWR>JF1=B6=MSM |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
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