|
|
|
Locus Name |
D15Mit107 |
Chromosome |
15
|
MGI Position (cM) |
49.0
|
Position (cM) |
40.45
|
Ensembl Chromosome |
15
|
Position (bp)(Length)(Chr.)(OLD) |
84214263-84214412 (150) (Chr.15)
|
Position (GRCm38) |
84386497-84386646 (150) (Chr.15)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
PGN2>129>MSM>B6=JF1>SM=SWR>A/J |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
PGN2
|
MSM
|
JF1
|
152 |
148 |
148 |
165 |
146 |
167 |
156 |
152 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
|
|
|