 |
 |
| |
Locus Name |
D4Mit15 |
| 染色体番号 |
4
|
| MGI Position (cM) |
42.6
|
| Position (cM) |
33.57
|
| Ensembl 染色体番号 |
4
|
| Position (bp)(Length)(Chr.)(OLD) |
91048480-91048764 (285) (Chr.4)
|
| Position (GRCm38) |
91556462-91556746 (284) (Chr.4)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
A/J>SK/Cam=CBA>PGN2>NJL>MSM=129=JF1>CAST=SWN>DBA=SWR=B6=NC=SM>BLG2 |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
| 290 |
290 |
290 |
331 |
353 |
349 |
290 |
290 |
348 |
349 |
339 |
287 |
329 |
331 |
331 |
329 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
|
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