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Locus table
  Locus Name D2Mit285
Chromosome 2
MGI Position (cM) 86.0
Position (cM) 74.49
Ensembl Chromosome 2
Position (bp)(Length)(Chr.)(OLD) 152548742-152548882 (141) (Chr.2)
Position (GRCm38) 152857301-152857441 (141) (Chr.2)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism 129>SM>SWR>B6>A/J>JF1=MSM>PGN2
Strains / Fragments (bp)
B6 SM SWR 129 A/J PGN2 MSM JF1
139 153 151 157 138 135 137 137

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image

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National Institute of Genetics