|
|
|
Locus Name |
D1Mit175 |
Chromosome |
1
|
MGI Position (cM) |
26.2
|
Position (cM) |
26.43
|
Ensembl Chromosome |
1
|
Position (bp)(Length)(Chr.)(OLD) |
48527586-48527719 (134) (Chr.1)
|
Position (GRCm38) |
48583041-48583174 (134) (Chr.1)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
PGN2=SWR>B6>A/J=129=SM>JF1=MSM |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
|
|
|