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Locus Name |
D7Mit170 |
Chromosome |
7
|
MGI Position (cM) |
23.0
|
Position (cM) |
19.11
|
Ensembl Chromosome |
7
|
Position (bp)(Length)(Chr.)(OLD) |
47270354-47270485 (132) (Chr.7)
|
Position (GRCm38) |
47402332-47402463 (132) (Chr.7)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
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|
Polymorphism |
JF1>SM=B6=A/J>SWR=129=PGN2=MSM |
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PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
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|
min |
|
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cycle 3 |
c |
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min |
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remarks |
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