 |
 |
| |
Locus Name |
D11Mit190 |
| Chromosome |
11
|
| MGI Position (cM) |
22.0
|
| Position (cM) |
22.4
|
| Ensembl Chromosome |
11
|
| Position (bp)(Length)(Chr.)(OLD) |
47624902-47625048 (147) (Chr.11)
|
| Position (GRCm38) |
47781479-47781625 (140) (Chr.11)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
NJL=MSM>CAST>SWN>JF1>PGN2>A/J=B6=CBA=DBA>SWR=SM=129>NC>BLG2>SK/Cam |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
| 147 |
146 |
146 |
146 |
147 |
147 |
147 |
142 |
151 |
132 |
167 |
136 |
163 |
167 |
162 |
165 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
|
|
|
|
|
