 |
 |
| |
Locus Name |
D15Mit199 |
| 染色体番号 |
15
|
| MGI Position (cM) |
10.8
|
| Position (cM) |
6.83
|
| Ensembl 染色体番号 |
15
|
| Position (bp)(Length)(Chr.)(OLD) |
12771341-12771465 (125) (Chr.15)
|
| Position (GRCm38) |
12826616-12826740 (125) (Chr.15)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
NC/Nga>JF1>MSM>SWR=129>A/J>PGN2>SM=B6=NC/jic |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
NC/Nga
|
NC/jic
|
PGN2
|
MSM
|
JF1
|
| 126 |
126 |
136 |
136 |
130 |
146 |
126 |
127 |
137 |
140 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
|
|
|
|
|
