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( Mouse Microsatellite Data Base of Japan )

Development of microsatellite markers has revolutionized mammalian genetics in the past decade. Simple sequence length polymorphisms (SSLPs) are easily typed by the polymerase chain reaction (PCR) with primers flanking the microsatellite sequences in the mammalian genome. In the mouse, the number of primer sets for SSLPs has reached almost 7,000, and they are commercially available. In addition to SSLPs, a mating scheme using interspecific and intersubspecific crosses facilitates high resolution mapping of genes in the mouse genome.

Mouse Microsatellite Data Base of Japan (MMDBJ) provides information about SSLPs among different mouse strains, focusing on strains derived from Japanese wild mouse, Mus musculus molossinus, which are genetically remote from the standard laboratory strains. Two molossinus-derived strains, MSM and JF1, are now widely used in gene mapping because of the high level of microsatellite polymorphisms for the standard laboratory strains and high reproduction ability in crosses with other strains. This data base includes PCR conditions for all entries of primer sets and keyword searches for the information.

As of June 2000, more than 1,700 entries of microsatellite loci have been made. The MMDBJ has been developed by contributions of many researchers in this field. We hope that scientists will continue to deposit their own data in the MMDBJ in order to make this data base more comprehensive and valuable. We are willing to accept any data of microsatellite polymorphisms irrespective of whether or not molossinus mouse strains are included.

Finally, we hope that the MMDBJ will be very useful in your future research.

This project is being conducted with the support of the "Genome Frontier Development Promotion" assisted by the Special Coordination Funds for Promoting Science and Technology of the Science and Technology Agency, to which we would like to express our deep gratitude.

National Institute of Genetics