FSI-Tg(Gt II)-msi1

Strain information
Strain ID TG1340
Strain Name FSI-Tg(Gt II)-msi1
Strain Type Transgenics
Characteristics Single transgene integration on chromosome 5, EGFP fluorescence in CNS, brain and in adult testis. See: Froschauer, A., Sprott, D., Gerwien, F. et al. Effective generation of transgenic reporter and gene trap lines of the medaka (Oryzias latipes) using the Ac/Ds transposon system. Transgenic Res 21, 149.162 (2012). https://doi.org/10.1007/s11248-011-9514-x
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Special affairs [Address] ■ The RECIPIENT shall refer the following publication in any PUBLICATIONS.
(Name of the Publication: FROSCHAUER, Alexander, et al. Effective generation of transgenic reporter and gene trap lines of the medaka (Oryzias latipes) using the Ac/Ds transposon system. Transgenic research, 2012, 21.1: 149-162. )
Category Transgenic strains
Organization National Institute for Basic Biology
Frozen sperm in NIBB
Deposited by Technische Universitaet Dresden
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Document (PDF) Tg(GT II)_transgene map
Document (PDF) Genotyping protocol_TG1340_FSI-Tg(GtII)-msi1
Image

TG1340_Tg(Gt II)_msi1
Characteristic embryonic fluorescence pattern in the gene trap line and FSI Tg(GtII)-msi1.
The msi1-GtII EGFP expression is weak and ubiquitous at 1 dpf (D). EGFP expressing cells in ventral brain regions lead to a diffuse fluorescence signal in the brain at 2 dpf (E) and 3 dpf (F)


/medaka