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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9935R-1 
 Symbol CG9935  Full Name CG9935 
 CG No CG9935  Old CG No CG9935 
 Synonyms CG9935, CT36399 
 Accession No (Link to NCBI) NM_143674.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGTTTGGGAGTTCCACCCTTG 
 Primer Seq. 3' 
aatctagaggtaccGAATCCCGAGAAACTTGTCGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGTTTGGGAG TTCCACCCTT GGTCAGAATT GGAGCTATAT TTTCAAACCA GCCTGGAATG 
0061 TATAACTCGG AGTTAGCGTT TAGATACGCT ATTCATAGAC TTAATATGGA CAAAAGCCTT 
0121 TTGCCTGAGA CTACCGTTGA TTACTATGTT GAATACGTAA ACCGATTTGA CTCTTTCGAG 
0181 ACAGTTCAAA AAGTGTGCAA ACTTATACGA GTTGGTGTTC AAGCTGTTTT TAGTCCAACT 
0241 GACTCTGTTT TGGCGACCCA TATAAATTCA ATTTGCGACG CACTGGACAT CCCAAACATA 
0301 GGCCGGTCTG CCCATGACTT TTCTATTAAC GTCTATCCAT CGAAGCAACT TGTAAATTAT 
0361 GCCTTTAACG ATGTAATACA GTATTTAAAT TGGACACGCT TTGGGATATT GCATGAAAAA 
0421 GAAAATGGAA TCATAAACTT GCATCAGCTA TCCCGGTCAT TTCACGGAGA AGTTCATATG 
0481 CGACAAGTTT CTCGGGATTC  
 in silico PCR Fragment 
0001 GGTTTGGGAG TTCCACCCTT GGTCAGAATT GGAGCTATAT TTTCAAACCA GCCTGGAATG 
0061 TATAACTCGG AGTTAGCGTT TAGATACGCT ATTCATAGAC TTAATATGGA CAAAAGCCTT 
0121 TTGCCTGAGA CTACCGTTGA TTACTATGTT GAATACGTAA ACCGATTTGA CTCTTTCGAG 
0181 ACAGTTCAAA AAGTGTGCAA ACTTATACGA GTTGGTGTTC AAGCTGTTTT TAGTCCAACT 
0241 GACTCTGTTT TGGCGACCCA TATAAATTCA ATTTGCGACG CACTGGACAT CCCAAACATA 
0301 GGCCGGTCTG CCCATGACTT TTCTATTAAC GTCTATCCAT CGAAGCAACT TGTAAATTAT 
0361 GCCTTTAACG ATGTAATACA GTATTTAAAT TGGACACGCT TTGGGATATT GCATGAAAAA 
0421 GAAAATGGAA TCATAAACTT GCATCAGCTA TCCCGGTCAT TTCACGGAGA AGTTCATATG 
0481 CGACAAGTTT CTCGGGATTC  
 Assemble Data 

9935R-1.IR_full       1   GGTTTGGGAGTTCCACCCTTGGTCAGAATTGGAGCTATATTTTCAAACCAGCCTGGAATG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9935R-1.in silico     1   GGTTTGGGAGTTCCACCCTTGGTCAGAATTGGAGCTATATTTTCAAACCAGCCTGGAATG 60


9935R-1.IR_full       61  TATAACTCGGAGTTAGCGTTTAGATACGCTATTCATAGACTTAATATGGACAAAAGCCTT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9935R-1.in silico     61  TATAACTCGGAGTTAGCGTTTAGATACGCTATTCATAGACTTAATATGGACAAAAGCCTT 120


9935R-1.IR_full       121 TTGCCTGAGACTACCGTTGATTACTATGTTGAATACGTAAACCGATTTGACTCTTTCGAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9935R-1.in silico     121 TTGCCTGAGACTACCGTTGATTACTATGTTGAATACGTAAACCGATTTGACTCTTTCGAG 180


9935R-1.IR_full       181 ACAGTTCAAAAAGTGTGCAAACTTATACGAGTTGGTGTTCAAGCTGTTTTTAGTCCAACT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9935R-1.in silico     181 ACAGTTCAAAAAGTGTGCAAACTTATACGAGTTGGTGTTCAAGCTGTTTTTAGTCCAACT 240


9935R-1.IR_full       241 GACTCTGTTTTGGCGACCCATATAAATTCAATTTGCGACGCACTGGACATCCCAAACATA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9935R-1.in silico     241 GACTCTGTTTTGGCGACCCATATAAATTCAATTTGCGACGCACTGGACATCCCAAACATA 300


9935R-1.IR_full       301 GGCCGGTCTGCCCATGACTTTTCTATTAACGTCTATCCATCGAAGCAACTTGTAAATTAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9935R-1.in silico     301 GGCCGGTCTGCCCATGACTTTTCTATTAACGTCTATCCATCGAAGCAACTTGTAAATTAT 360


9935R-1.IR_full       361 GCCTTTAACGATGTAATACAGTATTTAAATTGGACACGCTTTGGGATATTGCATGAAAAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9935R-1.in silico     361 GCCTTTAACGATGTAATACAGTATTTAAATTGGACACGCTTTGGGATATTGCATGAAAAA 420


9935R-1.IR_full       421 GAAAATGGAATCATAAACTTGCATCAGCTATCCCGGTCATTTCACGGAGAAGTTCATATG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9935R-1.in silico     421 GAAAATGGAATCATAAACTTGCATCAGCTATCCCGGTCATTTCACGGAGAAGTTCATATG 480


9935R-1.IR_full       481 CGACAAGTTTCTCGGGATTC 500
                          ||||||||||||||||||||
9935R-1.in silico     481 CGACAAGTTTCTCGGGATTC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_143674.1  CG9935-RA, transcript variant A (CG9935), mRNA 
100   482  0  0  1  NM_001042785.1  CG9935-RB, transcript variant B (CG9935), mRNA 
100   482  0  0  1  NM_001042786.1  CG9935-RC, transcript variant C (CG9935), mRNA 
0   0  2  4  3  NM_079240.2  CG7391-RA, transcript variant A (Clk), mRNA 
0   0  2  4  3  NM_001014574.1  CG7391-RF, transcript variant F (Clk), mRNA 
0   0  2  4  3  NM_001014575.1  CG7391-RE, transcript variant E (Clk), mRNA 
0   0  2  4  3  NM_001014576.1  CG7391-RD, transcript variant D (Clk), mRNA 
0   0  2  4  2  NM_206299.1  CG7391-RC, transcript variant C (Clk), mRNA 
0   0  2  4  2  NM_170628.1  CG7391-RB, transcript variant B (Clk), mRNA 
0   0  1  4  5  NM_058019.3  CG3181-RA (Ts), mRNA 
0   0  1  2  2  NM_137051.1  CG13337-RA (CG13337), mRNA 
0   0  0  7  3  NM_057215.3  CG4807-RB, transcript variant B (ab), mRNA 
0   0  0  7  3  NM_057214.3  CG4807-RA, transcript variant A (ab), mRNA 
0   0  0  7  2  NM_139443.1  CG13809-RA (osm-1), mRNA 
0   0  0  6  4  NM_139377.2  CG7955-RC, transcript variant C (CG7955), mRNA 
0   0  0  6  4  NM_167902.1  CG7955-RA, transcript variant A (CG7955), mRNA 
0   0  0  6  4  NM_167903.1  CG7955-RB, transcript variant B (CG7955), mRNA 
0   0  0  4  8  NM_167482.3  CG32580-RA (CG32580), mRNA 
0   0  0  4  6  NM_143768.2  CG12286-RA (kar), mRNA 
0   0  0  4  3  NM_001015505.1  CG17866-PA.3 (CG17866), mRNA 
0   0  0  4  3  NM_135923.2  CG18801-RA (Ku80), mRNA 
0   0  0  4  3  NM_143061.1  CG13648-RA (tnc), mRNA 
0   0  0  4  2  NM_169838.1  CG14296-RA, transcript variant A (endoA), mRNA 
0   0  0  4  2  NM_138767.2  CG14296-RB, transcript variant B (endoA), mRNA 
0   0  0  4  2  NM_138180.1  CG13881-RA (CG13881), mRNA 
0   0  0  3  7  NM_164374.1  CG18497-RA, transcript variant A (spen), mRNA 
0   0  0  3  7  NM_079979.2  CG18497-RB, transcript variant B (spen), mRNA 
0   0  0  3  7  NM_164375.1  CG18497-RC, transcript variant C (spen), mRNA 
0   0  0  3  4  NM_167434.2  CG5877-RB, transcript variant B (CG5877), mRNA 
0   0  0  3  4  NM_132786.2  CG5877-RA, transcript variant A (CG5877), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
++ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
     

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