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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9922R-2 |
| Symbol |
CG9922 |
Full Name |
CG9922 |
| CG No |
CG9922 |
Old CG No |
CG9922 |
| Synonyms |
NEST:bs28g09, BcDNA:RE55111, CG9922 |
| Accession No (Link to NCBI) |
NM_142072.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGACGGAAGCAGCGGAAATCAA |
| Primer Seq. 3' |
aatctagaggtaccCTGATGATGGCTTCCAAGGCG |
| Predicted Fragment Size |
366 |
| IR fragment full Seq |
0001 GACGGAAGCA GCGGAAATCA ACGGCAGAGA AGAAGTGGAG GATGACGAGC AGGATAAAAA
0061 GCAAAAGAAA TCCGATGTGA AACGACATGA CGACGGCGCC GCAGATTTGG AACGTGTGAC
0121 GGATTATGCC GAGGAAAAGG AGATCTCTGC GGACAATATA TCCAGCGCCG TGGAGCAGTT
0181 TGGCAACCAG CGTAACAAGG AGAACGAACT GCGAGTAGCC AAAGAGAAGG AGCTCCAGAA
0241 GGTCCAAGTC AAGAAGGAGG ACATTGAACT GATTATGAAC GAGCTGCTGG TTAGCAAGGC
0301 GCATNCCGAG AAAGTGCTCC GCGAGCAGAG CGGCGATGTG GTGGCCGCCT TGGAAGCCAT
0361 CATCAG |
| in silico PCR Fragment |
0001 GACGGAAGCA GCGGAAATCA ACGGCAGAGA AGAAGTGGAG GATGACGAGC AGGATAAAAA
0061 GCAAAAGAAA TCCGATGTGA AACGACATGA CGACGGCGCC GCAGATTTGG AACGTGTGAC
0121 GGATTATGCC GAGGAAAAGG AGATCTCTGC GGACAATATA TCCAGCGCCG TGGAGCAGTT
0181 TGGCAACCAG CGTAACAAGG AGAACGAACT GCGAGTAGCC AAAGAGAAGG AGCTCCAGAA
0241 GGTCCAAGTC AAGAAGGAGG ACATTGAACT GATTATGAAC GAGCTGCTGG TTAGCAAGGC
0301 GCATGCCGAG AAAGTGCTCC GCGAGCAGAG CGGCGATGTG GTGGCCGCCT TGGAAGCCAT
0361 CATCAG |
| Assemble Data |
9922R-2.IR_full 1 GACGGAAGCAGCGGAAATCAACGGCAGAGAAGAAGTGGAGGATGACGAGCAGGATAAAAA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9922R-2.in silico 1 GACGGAAGCAGCGGAAATCAACGGCAGAGAAGAAGTGGAGGATGACGAGCAGGATAAAAA 60
9922R-2.IR_full 61 GCAAAAGAAATCCGATGTGAAACGACATGACGACGGCGCCGCAGATTTGGAACGTGTGAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9922R-2.in silico 61 GCAAAAGAAATCCGATGTGAAACGACATGACGACGGCGCCGCAGATTTGGAACGTGTGAC 120
9922R-2.IR_full 121 GGATTATGCCGAGGAAAAGGAGATCTCTGCGGACAATATATCCAGCGCCGTGGAGCAGTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9922R-2.in silico 121 GGATTATGCCGAGGAAAAGGAGATCTCTGCGGACAATATATCCAGCGCCGTGGAGCAGTT 180
9922R-2.IR_full 181 TGGCAACCAGCGTAACAAGGAGAACGAACTGCGAGTAGCCAAAGAGAAGGAGCTCCAGAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9922R-2.in silico 181 TGGCAACCAGCGTAACAAGGAGAACGAACTGCGAGTAGCCAAAGAGAAGGAGCTCCAGAA 240
9922R-2.IR_full 241 GGTCCAAGTCAAGAAGGAGGACATTGAACTGATTATGAACGAGCTGCTGGTTAGCAAGGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9922R-2.in silico 241 GGTCCAAGTCAAGAAGGAGGACATTGAACTGATTATGAACGAGCTGCTGGTTAGCAAGGC 300
9922R-2.IR_full 301 GCATNCCGAGAAAGTGCTCCGCGAGCAGAGCGGCGATGTGGTGGCCGCCTTGGAAGCCAT 360
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
9922R-2.in silico 301 GCATGCCGAGAAAGTGCTCCGCGAGCAGAGCGGCGATGTGGTGGCCGCCTTGGAAGCCAT 360
9922R-2.IR_full 361 CATCAG 366
||||||
9922R-2.in silico 361 CATCAG 366
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
348 |
0 |
0 |
0 |
NM_142072.2 |
CG9922-RA (CG9922), mRNA |
| 0.57 |
2 |
4 |
2 |
2 |
NM_141703.1 |
CG12802-RA (CG12802), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_141669.2 |
CG8362-RA (nmdyn-D7), mRNA |
| 0 |
0 |
1 |
5 |
13 |
NM_132584.2 |
CG32654-RC (CG32654), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_165536.1 |
CG2146-RB, transcript variant B (didum), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_057838.3 |
CG2146-RA, transcript variant A (didum), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_165535.1 |
CG2146-RC, transcript variant C (didum), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_134988.1 |
CG15432-RA (CG15432), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_141123.1 |
CG7130-RA (CG7130), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_058072.3 |
CG8432-RA (Rep), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_143576.1 |
CG15547-RA (CG15547), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_139495.1 |
CG9973-RA (CG9973), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_079833.2 |
CG7917-RA (Nlp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_165375.2 |
CG31623-RA (dtr), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_142432.2 |
CG7985-RA (CG7985), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169249.1 |
CG31100-RA (CG31100), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_134792.3 |
CG4233-RC, transcript variant C (Got2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168835.1 |
CG17149-RB, transcript variant B (CG17149), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140937.2 |
CG17149-RA, transcript variant A (CG17149), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_057942.3 |
CG5227-RC, transcript variant C (sdk), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_134315.2 |
CG5227-RD, transcript variant D (sdk), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_134314.2 |
CG5227-RB, transcript variant B (sdk), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_057941.3 |
CG5227-RA, transcript variant A (sdk), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001032254.1 |
CG7969-RA, transcript variant A (Menl-2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001032253.1 |
CG7969-RB, transcript variant B (Menl-2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001032252.1 |
CG7969-RC, transcript variant C (Menl-2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001032257.1 |
CG7964-RA, transcript variant A (Menl-1), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_057915.3 |
CG9749-RA (Abi), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166120.1 |
CG30085-RA (CG30085), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169829.1 |
CG7702-RB, transcript variant B (CG7702), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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