NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9884R-2

Symbol oaf Full Name out at first

CG No CG9884 Old CG No CG9884

Synonyms l(2)22Fb, tnd, l(2)ND1, CG9884, oaf

Accession No (Link to NCBI) NM_057692.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAATTGCTGCGGTCGCGTCGT

Primer Seq. 3'

aatctagaggtaccCCTGCAGATGTCTGGTGATGG

Predicted Fragment Size

IR fragment full Seq

0001 CAATTGCTGC GGTCGCGTCG TCAGTAAAAA TCACTTTTTC AAGCACAGTC GCGCGTTTCT 
0061 GTNGTTCCTG TTGTGCAACT TAGTGATGAA CGCGGACgCA TTCTGCCCAC TCCCAGCTGC 
0121 TCATTAACGT CCAAAATCAG GGCGGCGAGG TGATCCAGGA GAGTATTACC TCCAACATTG 
0181 GCGAGGACCT GATAACGCTG GAGTTTCAGA AGACCGACGG AACGCATCAT CACCCAGGTC 
0241 ATCGACTTTC GCAATGAGGT TCAAATCCTC AAGGCTCTGG TTCTCGGCGA GGAGGAGCGT 
0301 gGCCAGAGCC AGTACCAGGT CAtgTNNTTC GCAACCAAGT TCAACAAGGG CGACTTCATC 
0361 TCCTCGGCGG CAATGGCCAA gcTGCGCCAG AAGAATCCGC ACACCATCCG CANTCCCGAG 
0421 GAGGACAAGG GCCGTGAGAC CTTCACCATG AGCAGCTGGG TACAgNTCAA CCGCTCGCTG 
0481 CCCATCACCA GACATCTGCA GG

in silico PCR Fragment

0001 CAATTGCTGC GGTCGCGTCG TCAGTAAAAA TCACTTTTTC AAGCACAGTC GCGCGTTTCT 
0061 GTGGTTCCTG CTGTGCAACT TAGTGATGAA CGCGGACGCA TTC-GCCCAC TCCCAGCTGC 
0121 TCATTAACGT CCAAAATCAG GGCGGCGAGG TGATCCAGGA GAGTATTACC TCCAACATTG 
0181 GCGAGGACCT GATAACGCTG GAGTTTCAGA AGACCGACGG AACGC-TCAT CACCCAGGTC 
0241 ATCGACTTTC GCAATGAGGT TCAAATCCTC AAGGCTCTGG TTCTCGGCGA GGAGGAGCGT 
0301 GGCCAGAGCC AGTACCAGGT CATGTGCTTC GCAACCAAGT TCAACAAGGG CGACTTCATC 
0361 TCCTCGGCGG CAATGGCCAA GCTGCGCCAG AAGAATCCGC ACACCATCCG CACTCCCGAG 
0421 GAGGACAAGG GCCGTGAGAC CTTCACCATG AGCAGCTGGG TACAGCTCAA CCGCTCGCTG 
0481 CCCATCACCA GACATCTGCA GG

Assemble Data


9884R-2.IR_full       1   CAATTGCTGCGGTCGCGTCGTCAGTAAAAATCACTTTTTCAAGCACAGTCGCGCGTTTCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9884R-2.in silico     1   CAATTGCTGCGGTCGCGTCGTCAGTAAAAATCACTTTTTCAAGCACAGTCGCGCGTTTCT 60


9884R-2.IR_full       61  GTNGTTCCTGTTGTGCAACTTAGTGATGAACGCGGACGCATTCTGCCCACTCCCAGCTGC 120
                          || ||||||| |||||||||||||||||||||||||||||||| ||||||||||||||||
9884R-2.in silico     61  GTGGTTCCTGCTGTGCAACTTAGTGATGAACGCGGACGCATTC-GCCCACTCCCAGCTGC 120


9884R-2.IR_full       121 TCATTAACGTCCAAAATCAGGGCGGCGAGGTGATCCAGGAGAGTATTACCTCCAACATTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9884R-2.in silico     121 TCATTAACGTCCAAAATCAGGGCGGCGAGGTGATCCAGGAGAGTATTACCTCCAACATTG 180


9884R-2.IR_full       181 GCGAGGACCTGATAACGCTGGAGTTTCAGAAGACCGACGGAACGCATCATCACCCAGGTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
9884R-2.in silico     181 GCGAGGACCTGATAACGCTGGAGTTTCAGAAGACCGACGGAACGC-TCATCACCCAGGTC 240


9884R-2.IR_full       241 ATCGACTTTCGCAATGAGGTTCAAATCCTCAAGGCTCTGGTTCTCGGCGAGGAGGAGCGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9884R-2.in silico     241 ATCGACTTTCGCAATGAGGTTCAAATCCTCAAGGCTCTGGTTCTCGGCGAGGAGGAGCGT 300


9884R-2.IR_full       301 GGCCAGAGCCAGTACCAGGTCATGTNNTTCGCAACCAAGTTCAACAAGGGCGACTTCATC 360
                          |||||||||||||||||||||||||  |||||||||||||||||||||||||||||||||
9884R-2.in silico     301 GGCCAGAGCCAGTACCAGGTCATGTGCTTCGCAACCAAGTTCAACAAGGGCGACTTCATC 360


9884R-2.IR_full       361 TCCTCGGCGGCAATGGCCAAGCTGCGCCAGAAGAATCCGCACACCATCCGCANTCCCGAG 420
                          |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
9884R-2.in silico     361 TCCTCGGCGGCAATGGCCAAGCTGCGCCAGAAGAATCCGCACACCATCCGCACTCCCGAG 420


9884R-2.IR_full       421 GAGGACAAGGGCCGTGAGACCTTCACCATGAGCAGCTGGGTACAGNTCAACCGCTCGCTG 480
                          ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
9884R-2.in silico     421 GAGGACAAGGGCCGTGAGACCTTCACCATGAGCAGCTGGGTACAGCTCAACCGCTCGCTG 480


9884R-2.IR_full       481 CCCATCACCAGACATCTGCAGG 502
                          ||||||||||||||||||||||
9884R-2.in silico     481 CCCATCACCAGACATCTGCAGG 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_057692.2	CG9884-RA, transcript variant A (oaf), mRNA
100	482	0	0	1	NM_175949.1	CG9884-RD, transcript variant D (oaf), mRNA
100	482	0	0	1	NM_164489.1	CG9884-RB, transcript variant B (oaf), mRNA
100	482	0	0	1	NM_164490.1	CG9884-RC, transcript variant C (oaf), mRNA
0	0	2	3	7	NM_132288.1	CG15365-RA (CG15365), mRNA
0	0	2	2	7	NM_136698.2	CG12134-RA, transcript variant A (CG12134), mRNA
0	0	2	2	7	NM_165725.2	CG12134-RB, transcript variant B (CG12134), mRNA
0	0	2	2	6	NM_165181.1	CG17927-RC, transcript variant C (Mhc), mRNA
0	0	2	2	6	NM_165182.1	CG17927-RG, transcript variant G (Mhc), mRNA
0	0	2	2	6	NM_165183.1	CG17927-RE, transcript variant E (Mhc), mRNA
0	0	2	2	6	NM_165184.1	CG17927-RJ, transcript variant J (Mhc), mRNA
0	0	2	2	6	NM_165185.1	CG17927-RF, transcript variant F (Mhc), mRNA
0	0	2	2	6	NM_165186.1	CG17927-RD, transcript variant D (Mhc), mRNA
0	0	2	2	6	NM_165187.1	CG17927-RA, transcript variant A (Mhc), mRNA
0	0	2	2	6	NM_165188.1	CG17927-RI, transcript variant I (Mhc), mRNA
0	0	2	2	6	NM_078863.4	CG17927-RH, transcript variant H (Mhc), mRNA
0	0	2	2	6	NM_165190.1	CG17927-RK, transcript variant K (Mhc), mRNA
0	0	2	2	6	NM_165191.1	CG17927-RL, transcript variant L (Mhc), mRNA
0	0	2	2	6	NM_165192.1	CG17927-RM, transcript variant M (Mhc), mRNA
0	0	2	2	6	NM_165189.1	CG17927-RB, transcript variant B (Mhc), mRNA
0	0	2	2	2	NM_135087.1	CG7371-RA (CG7371), mRNA
0	0	2	2	2	NM_141906.2	CG4860-RA (CG4860), mRNA
0	0	1	3	8	NM_079659.2	CG3937-RA, transcript variant A (cher), mRNA
0	0	1	3	8	NM_206502.1	CG3937-RD, transcript variant D (cher), mRNA
0	0	1	2	15	NM_141001.2	CG4365-RA, transcript variant A (CG4365), mRNA
0	0	1	2	15	NM_168873.1	CG4365-RC, transcript variant C (CG4365), mRNA
0	0	1	2	11	NM_168874.1	CG4365-RB, transcript variant B (CG4365), mRNA
0	0	1	2	7	NM_169061.1	CG4365-RB, transcript variant B (CG4365), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA
0	0	1	2	7	NM_169060.1	CG4365-RB, transcript variant B (CG4365), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA
0	0	1	2	5	NM_132061.1	CG5937-RA (CG5937), mRNA

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