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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9781R-1 
 Symbol obst-G  Full Name obstructor-G 
 CG No CG9781  Old CG No CG9781 
 Synonyms obst-G, CG9781 
 Accession No (Link to NCBI) NM_140272.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGTGCTGATGGAAACGCTGTGA 
 Primer Seq. 3' 
aatctagaggtaccATCGCTGCGATGAGCAACAAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NTGCTGATGG AAACGCTGTG ACCGGAACTT GTGAAAAGAA TACGTTGTTT AATCCTTTAN 
0061 ACGTTGCACT GCGATGATCC TGACAATGTG GATTGCATTT TCGATGGCAA GGATAATATT 
0121 GTTGACGACA CGTCAAGTTC TGAAAGCGAC GAAGACGATG ATGAGGAAAT GGCCAAAACT 
0181 GATCCTCCCG TTACTGTCAA AGCGACGAAA AAGCCGCGTC CTACAACCCT TGACAAAATG 
0241 TGTGCTGGCA AGAAGGATGG AGTGATGCTT ACAAAGAACG GGTCCTGCCA GGAATATTAT 
0301 GTCTGCAAAG CAAAAAAGCC CCATCTGCGC TCTTGTCCTG ACAAACAACA TTTTAGTCCA 
0361 ACTCGTCGCA TTTGCATGAA GGCCTCAGAA GCCAAGTGTT CGGGTGGAAC CCGAGAAAAT 
0421 AAAGAATCAG ATGGTCCTGC CACCACGGGA GGAGTGTGTT CAGATGAAAA GGAAAACTCT 
0481 CTTGTTgCTC ATCGCAGCGA T 
 in silico PCR Fragment 
0001 GTGCTGATGG AAACGCTGTG ACCGGAACTT GTGAAAAGAA TACGTTGTTT AATCCTTTA- 
0061 ACGTTGCACT GCGATGATCC TGACAATGTG GATTGCATTT TCGATGGCAA GGATAATATT 
0121 GTTGACGACA CGTCAAGTTC TGAAAGCGAC GAAGACGATG ATGAGGAAAT GGCCAAAACT 
0181 GATCCTCCCG TTACTGTCAA AGCGACGAAA AAGCCGCGTC CTACAACCCT TGACAAAATG 
0241 TGTGCTGGCA AGAAGGATGG AGTGATGCTT ACAAAGAACG GGTCCTGCCA GGAATATTAT 
0301 GTCTGCAAAG CAAAAAAGCC CCATCTGCGC TCTTGTCCTG ACAAACAACA TTTTAGTCCA 
0361 ACTCGTCGCA TTTGCATGAA GGCCTCAGAA GCCAAGTGTT CGGGTGGAAC CCGAGAAAAT 
0421 AAAGAATCAG ATGGTCCTGC CACCACGGGA GGAGTGTGTT CAGATGAAAA GGAAAACTCT 
0481 CTTGTTGCTC ATCGCAGCGA T 
 Assemble Data 

9781R-1.IR_full        1   TGCTGATGGAAACGCTGTGACCGGAACTTGTGAAAAGAATACGTTGTTTAATCCTTTAN 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
9781R-1.in silico      1   TGCTGATGGAAACGCTGTGACCGGAACTTGTGAAAAGAATACGTTGTTTAATCCTTTA- 59


9781R-1.IR_full       61  ACGTTGCACTGCGATGATCCTGACAATGTGGATTGCATTTTCGATGGCAAGGATAATATT 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9781R-1.in silico     61  ACGTTGCACTGCGATGATCCTGACAATGTGGATTGCATTTTCGATGGCAAGGATAATATT 119


9781R-1.IR_full       121 GTTGACGACACGTCAAGTTCTGAAAGCGACGAAGACGATGATGAGGAAATGGCCAAAACT 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9781R-1.in silico     121 GTTGACGACACGTCAAGTTCTGAAAGCGACGAAGACGATGATGAGGAAATGGCCAAAACT 179


9781R-1.IR_full       181 GATCCTCCCGTTACTGTCAAAGCGACGAAAAAGCCGCGTCCTACAACCCTTGACAAAATG 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9781R-1.in silico     181 GATCCTCCCGTTACTGTCAAAGCGACGAAAAAGCCGCGTCCTACAACCCTTGACAAAATG 239


9781R-1.IR_full       241 TGTGCTGGCAAGAAGGATGGAGTGATGCTTACAAAGAACGGGTCCTGCCAGGAATATTAT 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9781R-1.in silico     241 TGTGCTGGCAAGAAGGATGGAGTGATGCTTACAAAGAACGGGTCCTGCCAGGAATATTAT 299


9781R-1.IR_full       301 GTCTGCAAAGCAAAAAAGCCCCATCTGCGCTCTTGTCCTGACAAACAACATTTTAGTCCA 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9781R-1.in silico     301 GTCTGCAAAGCAAAAAAGCCCCATCTGCGCTCTTGTCCTGACAAACAACATTTTAGTCCA 359


9781R-1.IR_full       361 ACTCGTCGCATTTGCATGAAGGCCTCAGAAGCCAAGTGTTCGGGTGGAACCCGAGAAAAT 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9781R-1.in silico     361 ACTCGTCGCATTTGCATGAAGGCCTCAGAAGCCAAGTGTTCGGGTGGAACCCGAGAAAAT 419


9781R-1.IR_full       421 AAAGAATCAGATGGTCCTGCCACCACGGGAGGAGTGTGTTCAGATGAAAAGGAAAACTCT 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9781R-1.in silico     421 AAAGAATCAGATGGTCCTGCCACCACGGGAGGAGTGTGTTCAGATGAAAAGGAAAACTCT 479


9781R-1.IR_full       481 CTTGTTGCTCATCGCAGCGAT 500
                          |||||||||||||||||||||
9781R-1.in silico     481 CTTGTTGCTCATCGCAGCGAT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140272.1  CG9781-RA (CG9781), mRNA 
0   0  6  7  7  NM_143365.2  CG10009-RA (Noa36), mRNA 
0   0  4  4  11  NM_168072.1  CG15010-RA, transcript variant A (ago), mRNA 
0   0  4  4  11  NM_168073.1  CG15010-RB, transcript variant B (ago), mRNA 
0   0  4  4  11  NM_079198.2  CG15010-RC, transcript variant C (ago), mRNA 
0   0  3  3  11  NM_167218.1  CG32685-RC (CG32685), mRNA 
0   0  2  5  11  NM_001038966.1  CG33967-RA (CG33967), mRNA 
0   0  2  3  5  NM_206099.1  CG8878-RB, transcript variant B (CG8878), mRNA 
0   0  2  3  5  NM_136889.2  CG8878-RA, transcript variant A (CG8878), mRNA 
0   0  2  2  12  NM_166882.1  CG32813-RC, transcript variant C (CG32813), mRNA 
0   0  2  2  12  NM_166884.2  CG32813-RB, transcript variant B (CG32813), mRNA 
0   0  2  2  12  NM_130553.2  CG32813-RA, transcript variant A (CG32813), mRNA 
0   0  2  2  12  NM_166881.1  CG32813-RE, transcript variant E (CG32813), mRNA 
0   0  2  2  12  NM_166885.1  CG32813-RD, transcript variant D (CG32813), mRNA 
0   0  2  2  12  NM_166883.1  CG32813-RF, transcript variant F (CG32813), mRNA 
0   0  2  2  6  NM_168629.2  CG32149-RC, transcript variant C (RhoGAP71E), mRNA 
0   0  2  2  6  NM_140527.2  CG32149-RA, transcript variant A (RhoGAP71E), mRNA 
0   0  2  2  6  NM_168630.1  CG32149-RB, transcript variant B (RhoGAP71E), mRNA 
0   0  2  2  5  NM_168627.1  CG7656-RC, transcript variant C (CG7656), mRNA 
0   0  2  2  5  NM_168628.1  CG7656-RA, transcript variant A (CG7656), mRNA 
0   0  2  2  5  NM_140526.3  CG7656-RB, transcript variant B (CG7656), mRNA 
0   0  2  2  4  NM_206004.1  CG10719-RC, transcript variant C (brat), mRNA 
0   0  2  2  4  NM_134302.1  CG10719-RB, transcript variant B (brat), mRNA 
0   0  2  2  4  NM_057597.2  CG10719-RA, transcript variant A (brat), mRNA 
0   0  2  2  3  NM_132133.1  CG3044-RA (CG3044), mRNA 
0   0  1  7  11  NM_080341.2  CG12737-RA, transcript variant A (Crag), mRNA 
0   0  1  7  11  NM_167164.2  CG12737-RB, transcript variant B (Crag), mRNA 
0   0  1  5  15  NM_132420.2  CG32676-RA (CG32676), mRNA 
0   0  1  4  4  NM_080075.2  CG32400-RA (Lcp65Ab1), mRNA 
0   0  1  4  4  NM_176291.1  CG18773-RA (Lcp65Ab2), mRNA 

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