NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9747R-1

Symbol CG9747 Full Name CG9747

CG No CG9747 Old CG No CG9747

Synonyms CG9747

Accession No (Link to NCBI) NM_143522.2

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAGTGTGCAGCAAGACGCCAG

Primer Seq. 3'

aatctagaggtaccGATGGATCGCAGCACGGTGTT

Predicted Fragment Size

IR fragment full Seq

0001 NAGTGTGCAG CAAGACGCCA GCCATCGCGA CGGACTCCAG TCCTCCGCTT GTGGCCAGGA 
0061 gGTCGAAGTT CCCCAATGGA GACGCCCAGC AGCAGGTGCG CAGGGGAGAG GACTCCTCGC 
0121 CCGAGCTGCC GCCCCGTGGC AAGCTCCAAC TGCCCCCGAA GCGGNAGGAG CTGCCCAGGT 
0181 CGGTGGTGCC CAAGGAGATC GCCAATCATG TGCCCAAGGA TGCAATTGAT CTCGAGGAGT 
0241 ACACACCGCG ACCTTCGTCG GAGATCGAGT GTTGGGTTGG CAGTTTCAGG CTCCGCTCAA 
0301 GTGGGACAAA GTCATCCAGA TCTCGCTCCT GCACATCGTG GCCGGAATCT GCCTDCTGAC 
0361 GTATCCGCTG CGGGAGCTCA ATCCATACAC CACCATCTGG TCCTTCTTCG TTGGCGGCGT 
0421 GGCCGGATTC GGAGTTACGG NCCGGAGCCC ATAGGTTCTG GACCCACAAG AGCTACAAGG 
0481 CCAACACCGT GCTGCGATCC ATC

in silico PCR Fragment

0001 GAGTGTGCAG CAAGACGCCA GCCATCGCGA CGGACTCCAG TCCTCCGCTT GTGGCCAGGA 
0061 GGTCGAAGTT CCCCAATGGA GACGCCCAGC AGCAGGTGCG CAGGGGAGAG GACTCCTCGC 
0121 CCGAGCTGCC GCCCCGTGGC AAGCTCCAAC TGCCCCCGAA GCGGGAGGAG CTGCCCAGGT 
0181 CGGTGGTGCC CAAGGAGATC GCCAATCATG TGCCCAAGGA TGCAATTGAT CTCGAGGAGT 
0241 ACAC-CCGC- ACCTTCGTCG GAGATCGAGT GTTGGGTTGG CAGTTTCAGG CTCCGCTCAA 
0301 GTGGGACAAA GTCATCCAGA TCTCGCTCCT GCACATCGTG GCCGGAATCT GCCTGCTGAC 
0361 GTATCCGCTG CGGGAGCTCA ATCCATACAC CACCATCTGG TCCTTCTTCG TTGGCGGCGT 
0421 GGCCGGATTC GGAGTTACGG -CCGGAGCCC ATAGGTTCTG GACCCACAAG AGCTACAAGG 
0481 CCAACACCGT GCTGCGATCC ATC

Assemble Data


9747R-1.IR_full        1   AGTGTGCAGCAAGACGCCAGCCATCGCGACGGACTCCAGTCCTCCGCTTGTGGCCAGGA 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9747R-1.in silico      1   AGTGTGCAGCAAGACGCCAGCCATCGCGACGGACTCCAGTCCTCCGCTTGTGGCCAGGA 59


9747R-1.IR_full       61  GGTCGAAGTTCCCCAATGGAGACGCCCAGCAGCAGGTGCGCAGGGGAGAGGACTCCTCGC 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9747R-1.in silico     61  GGTCGAAGTTCCCCAATGGAGACGCCCAGCAGCAGGTGCGCAGGGGAGAGGACTCCTCGC 119


9747R-1.IR_full       121 CCGAGCTGCCGCCCCGTGGCAAGCTCCAACTGCCCCCGAAGCGGNAGGAGCTGCCCAGGT 179
                          |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
9747R-1.in silico     121 CCGAGCTGCCGCCCCGTGGCAAGCTCCAACTGCCCCCGAAGCGGGAGGAGCTGCCCAGGT 179


9747R-1.IR_full       181 CGGTGGTGCCCAAGGAGATCGCCAATCATGTGCCCAAGGATGCAATTGATCTCGAGGAGT 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9747R-1.in silico     181 CGGTGGTGCCCAAGGAGATCGCCAATCATGTGCCCAAGGATGCAATTGATCTCGAGGAGT 239


9747R-1.IR_full       241 ACACACCGCGACCTTCGTCGGAGATCGAGTGTTGGGTTGGCAGTTTCAGGCTCCGCTCAA 299
                          |||| |||| ||||||||||||||||||||||||||||||||||||||||||||||||||
9747R-1.in silico     241 ACAC-CCGC-ACCTTCGTCGGAGATCGAGTGTTGGGTTGGCAGTTTCAGGCTCCGCTCAA 299


9747R-1.IR_full       301 GTGGGACAAAGTCATCCAGATCTCGCTCCTGCACATCGTGGCCGGAATCTGCCTDCTGAC 359
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
9747R-1.in silico     301 GTGGGACAAAGTCATCCAGATCTCGCTCCTGCACATCGTGGCCGGAATCTGCCTGCTGAC 359


9747R-1.IR_full       361 GTATCCGCTGCGGGAGCTCAATCCATACACCACCATCTGGTCCTTCTTCGTTGGCGGCGT 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9747R-1.in silico     361 GTATCCGCTGCGGGAGCTCAATCCATACACCACCATCTGGTCCTTCTTCGTTGGCGGCGT 419


9747R-1.IR_full       421 GGCCGGATTCGGAGTTACGGNCCGGAGCCCATAGGTTCTGGACCCACAAGAGCTACAAGG 479
                          |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
9747R-1.in silico     421 GGCCGGATTCGGAGTTACGG-CCGGAGCCCATAGGTTCTGGACCCACAAGAGCTACAAGG 479


9747R-1.IR_full       481 CCAACACCGTGCTGCGATCCATC 502
                          |||||||||||||||||||||||
9747R-1.in silico     481 CCAACACCGTGCTGCGATCCATC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_143522.2	CG9747-RA (CG9747), mRNA
0	0	4	5	2	NM_136090.2	CG15173-RA (CG15173), mRNA
0	0	3	3	3	NM_079845.2	CG7951-RA (sima), mRNA
0	0	2	3	2	NM_165291.1	CG17544-RC, transcript variant C (CG17544), mRNA
0	0	2	3	2	NM_136117.2	CG17544-RA, transcript variant A (CG17544), mRNA
0	0	2	3	2	NM_165290.1	CG17544-RB, transcript variant B (CG17544), mRNA
0	0	2	2	5	NM_136732.1	CG12904-RA (CG12904), mRNA
0	0	1	5	2	NM_139891.3	CG7462-RC, transcript variant C (Ank2), mRNA
0	0	1	4	6	NM_168228.1	CG32375-RA (CG32375), mRNA
0	0	1	4	3	NM_168382.1	CG6721-RB, transcript variant B (Gap1), mRNA
0	0	1	4	3	NM_079290.2	CG6721-RA, transcript variant A (Gap1), mRNA
0	0	1	4	2	NM_136211.3	CG9320-RA (CG9320), mRNA
0	0	1	3	4	NM_135749.2	CG5983-RA, transcript variant A (ACXC), mRNA
0	0	1	3	4	NM_001038813.1	CG5983-RB, transcript variant B (ACXC), mRNA
0	0	1	2	11	NM_132061.1	CG5937-RA (CG5937), mRNA
0	0	1	2	5	NM_167620.2	CG32547-RA (CG32547), mRNA
0	0	1	2	3	NM_167502.2	CG12223-RD, transcript variant D (Dsp1), mRNA
0	0	1	2	3	NM_080715.3	CG12223-RC, transcript variant C (Dsp1), mRNA
0	0	1	2	3	NM_139598.2	CG14989-RB (CG14989), mRNA
0	0	1	2	3	NM_206762.1	CG12223-RE, transcript variant E (Dsp1), mRNA
0	0	1	2	3	NM_167503.2	CG12223-RA, transcript variant A (Dsp1), mRNA
0	0	1	2	3	NM_167504.1	CG12223-RB, transcript variant B (Dsp1), mRNA
0	0	1	2	3	NM_139704.1	CG10635-RA (CG10635), mRNA
0	0	1	2	2	NM_132223.2	CG10959-RA (CG10959), mRNA
0	0	0	6	9	NM_141500.1	CG2767-RA (CG2767), mRNA
0	0	0	5	5	NM_080421.3	CG18730-RA (Amy-p), mRNA
0	0	0	5	5	NM_079044.1	CG17876-RA (Amy-d), mRNA
0	0	0	5	4	NM_138067.1	CG15874-RA (CG15874), mRNA
0	0	0	4	8	NM_165823.1	CG30028-RA (gammaTry), mRNA
0	0	0	4	8	NM_132213.1	CG2260-RA (CG2260), mRNA

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