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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9705R-3 
 Symbol CG9705  Full Name CG9705 
 CG No CG9705  Old CG No CG9705 
 Synonyms CG9705 
 Accession No (Link to NCBI) NM_140663.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees male pupal semi-lethal, female pupal semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGACCGAACCAAGGACGCCC 
 Primer Seq. 3' 
aatctagaggtaccGGCGAGGAGCCACCATAGAAT 
 Predicted Fragment Size 
422 
 IR fragment full Seq 
0001 ATGACCGAAC CAAGGACGCC CGAGAAGCTG CTGGCCGCCA AGCCGCCGGT TTTACACCAC 
0061 AACAGCCATA GTCCCAATGC CTCGCTGCAG CTGCCCAGTC CCATCATCAC GAAGGCGCAC 
0121 TCGCACGGCC TCGACCTCCG CTCGGGCCTT GGAAAATCCG GTTGTGACCG GTATGGTGAA 
0181 ATCCTTCAGC CGCACCAAAG GACACGGCTT CATTACACCC AACGCCGGCG GAGAGGACGT 
0241 TTTTTGCCAC GTTTCCGACA TCGAAGGTGA ATATGTGCCC ATGCCCGGGG ACGAGGTCAA 
0301 ATACCGTCTG TGCGCCATTC CGCCCAAGTA TGAGAAGCAC CTAGGCCGTG CACAGTGCTA 
0361 GATTAGCCAC CTGACAGCCG GAGGTGCACC ACAAGTGGGA GGAGCCATTC TATGGTGGCT 
0421 CCTCGCC 
 in silico PCR Fragment 
0001 ATGACCGAAC CAAGGACGCC CGAGAAGCTG CTGGCCGCCA AGCCGCCGGT TTTACACCAC 
0061 AACAGCCATA GTCCCAATGC CTCGCTGCAG CTGCCCAGTC CCATCATCAC G-AGGCGCAC 
0121 TCGCACGGCC TCGACCTCCG CTCGGGCCTT GGAAAATCCG GTTGTGACCG GTATGGTGAA 
0181 ATCCTTCAGC CGCACCAAAG GACACGGCTT CATTACACCC AACGCCGGCG GAGAGGACGT 
0241 TTTTTGCCAC GTTTCCGACA TCGAAGGTGA ATATGTGCCC ATGCCCGGGG ACGAGGTCAA 
0301 ATACCGTCTG TGCGCCATTC CGCCCAAGTA TGAGAAGCAC C-AGGCCGTG CAC-GTGC-A 
0361 GATTAGCCAC CTGAC-GCCG GAGGTGCACC ACAAGTGGGA GGAGCCATTC TATGGTGGCT 
0421 CCTCGCC 
 Assemble Data 

9705R-3.IR_full       1   ATGACCGAACCAAGGACGCCCGAGAAGCTGCTGGCCGCCAAGCCGCCGGTTTTACACCAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9705R-3.in silico     1   ATGACCGAACCAAGGACGCCCGAGAAGCTGCTGGCCGCCAAGCCGCCGGTTTTACACCAC 60


9705R-3.IR_full       61  AACAGCCATAGTCCCAATGCCTCGCTGCAGCTGCCCAGTCCCATCATCACGAAGGCGCAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
9705R-3.in silico     61  AACAGCCATAGTCCCAATGCCTCGCTGCAGCTGCCCAGTCCCATCATCACG-AGGCGCAC 120


9705R-3.IR_full       121 TCGCACGGCCTCGACCTCCGCTCGGGCCTTGGAAAATCCGGTTGTGACCGGTATGGTGAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9705R-3.in silico     121 TCGCACGGCCTCGACCTCCGCTCGGGCCTTGGAAAATCCGGTTGTGACCGGTATGGTGAA 180


9705R-3.IR_full       181 ATCCTTCAGCCGCACCAAAGGACACGGCTTCATTACACCCAACGCCGGCGGAGAGGACGT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9705R-3.in silico     181 ATCCTTCAGCCGCACCAAAGGACACGGCTTCATTACACCCAACGCCGGCGGAGAGGACGT 240


9705R-3.IR_full       241 TTTTTGCCACGTTTCCGACATCGAAGGTGAATATGTGCCCATGCCCGGGGACGAGGTCAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9705R-3.in silico     241 TTTTTGCCACGTTTCCGACATCGAAGGTGAATATGTGCCCATGCCCGGGGACGAGGTCAA 300


9705R-3.IR_full       301 ATACCGTCTGTGCGCCATTCCGCCCAAGTATGAGAAGCACCTAGGCCGTGCACAGTGCTA 360
                          ||||||||||||||||||||||||||||||||||||||||| ||||||||||| |||| |
9705R-3.in silico     301 ATACCGTCTGTGCGCCATTCCGCCCAAGTATGAGAAGCACC-AGGCCGTGCAC-GTGC-A 360


9705R-3.IR_full       361 GATTAGCCACCTGACAGCCGGAGGTGCACCACAAGTGGGAGGAGCCATTCTATGGTGGCT 420
                          ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
9705R-3.in silico     361 GATTAGCCACCTGAC-GCCGGAGGTGCACCACAAGTGGGAGGAGCCATTCTATGGTGGCT 420


9705R-3.IR_full       421 CCTCGCC 427
                          |||||||
9705R-3.in silico     421 CCTCGCC 427



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   404  0  0  0  NM_168683.1  CG9705-RB, transcript variant B (CG9705), mRNA 
100   404  0  0  0  NM_140663.2  CG9705-RA, transcript variant A (CG9705), mRNA 
0.24   1  7  2  2  NM_164645.1  CG31915-RA (CG31915), mRNA 
0   0  3  7  2  NM_166125.2  CG18255-RA, transcript variant A (Strn-Mlck), mRNA 
0   0  3  7  2  NM_166126.1  CG18255-RC, transcript variant C (Strn-Mlck), mRNA 
0   0  3  7  2  NM_166127.1  CG18255-RF, transcript variant F (Strn-Mlck), mRNA 
0   0  3  7  2  NM_079030.2  CG18255-RB, transcript variant B (Strn-Mlck), mRNA 
0   0  3  7  2  NM_166128.1  CG18255-RG, transcript variant G (Strn-Mlck), mRNA 
0   0  2  8  7  NM_139726.1  CG17334-RA (lin-28), mRNA 
0   0  2  5  3  NM_135451.1  CG3818-RA (CG3818), mRNA 
0   0  2  5  2  NM_134856.1  CG15398-RA (CG15398), mRNA 
0   0  2  2  13  NM_144448.2  CG1915-RC, transcript variant C (sls), mRNA 
0   0  2  2  4  NM_143745.2  CG18389-RA (Eip93F), mRNA 
0   0  2  2  4  NM_166641.1  CG2987-RB, transcript variant B (alpha-catenin-related), mRNA 
0   0  2  2  4  NM_143816.2  CG2987-RA, transcript variant A (alpha-catenin-related), mRNA 
0   0  1  7  6  NM_079176.1  CG12008-RA, transcript variant A (kst), mRNA 
0   0  1  7  6  NM_206266.1  CG12008-RB, transcript variant B (kst), mRNA 
0   0  1  7  6  NM_206265.1  CG12008-RC, transcript variant C (kst), mRNA 
0   0  1  4  4  NM_139990.2  CG5971-RA (CG5971), mRNA 
0   0  1  4  4  NM_206080.1  CG2204-RH, transcript variant H (G-oalpha47A), mRNA 
0   0  1  4  3  NM_176127.2  CG2204-RG, transcript variant G (G-oalpha47A), mRNA 
0   0  1  4  3  NM_078960.4  CG2204-RA, transcript variant A (G-oalpha47A), mRNA 
0   0  1  4  3  NM_176126.2  CG2204-RF, transcript variant F (G-oalpha47A), mRNA 
0   0  1  4  3  NM_206079.1  CG2204-RI, transcript variant I (G-oalpha47A), mRNA 
0   0  1  4  3  NM_176125.2  CG2204-RE, transcript variant E (G-oalpha47A), mRNA 
0   0  1  4  3  NM_176124.2  CG2204-RD, transcript variant D (G-oalpha47A), mRNA 
0   0  1  4  3  NM_165773.2  CG2204-RC, transcript variant C (G-oalpha47A), mRNA 
0   0  1  4  3  NM_165772.3  CG2204-RB, transcript variant B (G-oalpha47A), mRNA 
0   0  1  3  11  NM_058124.2  CG5772-RA (Sur), mRNA 
0   0  1  3  5  NM_167487.1  CG32577-RA (disco-r), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+- 
DC
+ 
DM
+- 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
++ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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