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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9663R-3 
 Symbol CG9663  Full Name CG9663 
 CG No CG9663  Old CG No CG9663 
 Synonyms CG9663 
 Accession No (Link to NCBI) NM_134916.3 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATGTCATCCGGTTGAACCAC 
 Primer Seq. 3' 
aatctagaggtaccGCACTAAGTTCTCCCGACTTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NATGTCATCC GGTTGAACCA CCTAAAGTCC AGCTCGGAAA CGCATCTGCC ATCGCGGGGA 
0061 TCTGGAACGG GGTCTGGAGC ACTACCACGT CCCACGACAC CCGTGCCCTC CAAGAAACTG 
0121 TCGTCCAAAC GACCTCCACA CTTGACCCTC AACATAAAGG CCAAGTCCCA CGATGCTCCG 
0181 CTGGCCGAGA CGGAAAACAC GGTTCTCTCG ACCAGTGAAT CTGTCTATGG AACTCCGCTG 
0241 GGCGCCACAC CCGTTTCGTC ACCTGCCAAC CAAAGTCAGC GCAGTCAGGC GAGCCAGCAT 
0301 AGTGACGAAA GCCGCAACAC CAGCANGCAC CGCCACCAGC GGAGGATCCA TATTCCCCCA 
0361 CGAACAGTAC AAAACCATCA AGAAGATCAA CATTGGCTTC GAGAATATAC GCTACACTAC 
0421 CAAATTCGGA GTCTTCCAGA GGGAAACCAA GGATGTGCTA ATGGGTTTAA CGGGCTACTT 
0481 CAAGTCGGGA GAACTTAGTG C 
 in silico PCR Fragment 
0001 GATGTCATCC GGTTGAACCA CCTAAAGTCC AGCTCGGAAA CGCATCTGCC ATCGCGGGGA 
0061 TCTGGAACGG GGTCTGGAGC ACTACCACGT CCCACGACAC CCGTGCCCTC CAAGAAACTG 
0121 TCGTCCAAAC GACCTCCACA CTTGACCCTC AACATAAAGG CCAAGTCCCA CGATGCTCCG 
0181 CTGGCCGAGA CGGAAAACAC GGTTCTCTCG ACCAGTGAAT CTGTCTATGG AACTCCGCTG 
0241 GGCGCCACAC CCGTTTCGTC ACCTGCCAAC CAAAGTCAGC GCAGTCAGGC GAGCCAGCAT 
0301 AGTGACGAAA GCCGCAACAC CAGCA-GCAC CGCCACCAGC GGAGGATCCA TATTCCCCCA 
0361 CGAACAGTAC AAAACCATCA AGAAGATCAA CATTGGCTTC GAGAATATAC GCTACACTAC 
0421 CAAATTCGGA GTCTTCCAGA GGGAAACCAA GGATGTGCTA ATGGGTTTAA CGGGCTACTT 
0481 CAAGTCGGGA GAACTTAGTG C 
 Assemble Data 

9663R-3.IR_full        1   ATGTCATCCGGTTGAACCACCTAAAGTCCAGCTCGGAAACGCATCTGCCATCGCGGGGA 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9663R-3.in silico      1   ATGTCATCCGGTTGAACCACCTAAAGTCCAGCTCGGAAACGCATCTGCCATCGCGGGGA 59


9663R-3.IR_full       61  TCTGGAACGGGGTCTGGAGCACTACCACGTCCCACGACACCCGTGCCCTCCAAGAAACTG 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9663R-3.in silico     61  TCTGGAACGGGGTCTGGAGCACTACCACGTCCCACGACACCCGTGCCCTCCAAGAAACTG 119


9663R-3.IR_full       121 TCGTCCAAACGACCTCCACACTTGACCCTCAACATAAAGGCCAAGTCCCACGATGCTCCG 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9663R-3.in silico     121 TCGTCCAAACGACCTCCACACTTGACCCTCAACATAAAGGCCAAGTCCCACGATGCTCCG 179


9663R-3.IR_full       181 CTGGCCGAGACGGAAAACACGGTTCTCTCGACCAGTGAATCTGTCTATGGAACTCCGCTG 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9663R-3.in silico     181 CTGGCCGAGACGGAAAACACGGTTCTCTCGACCAGTGAATCTGTCTATGGAACTCCGCTG 239


9663R-3.IR_full       241 GGCGCCACACCCGTTTCGTCACCTGCCAACCAAAGTCAGCGCAGTCAGGCGAGCCAGCAT 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9663R-3.in silico     241 GGCGCCACACCCGTTTCGTCACCTGCCAACCAAAGTCAGCGCAGTCAGGCGAGCCAGCAT 299


9663R-3.IR_full       301 AGTGACGAAAGCCGCAACACCAGCANGCACCGCCACCAGCGGAGGATCCATATTCCCCCA 359
                          ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
9663R-3.in silico     301 AGTGACGAAAGCCGCAACACCAGCA-GCACCGCCACCAGCGGAGGATCCATATTCCCCCA 359


9663R-3.IR_full       361 CGAACAGTACAAAACCATCAAGAAGATCAACATTGGCTTCGAGAATATACGCTACACTAC 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9663R-3.in silico     361 CGAACAGTACAAAACCATCAAGAAGATCAACATTGGCTTCGAGAATATACGCTACACTAC 419


9663R-3.IR_full       421 CAAATTCGGAGTCTTCCAGAGGGAAACCAAGGATGTGCTAATGGGTTTAACGGGCTACTT 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9663R-3.in silico     421 CAAATTCGGAGTCTTCCAGAGGGAAACCAAGGATGTGCTAATGGGTTTAACGGGCTACTT 479


9663R-3.IR_full       481 CAAGTCGGGAGAACTTAGTGC 500
                          |||||||||||||||||||||
9663R-3.in silico     481 CAAGTCGGGAGAACTTAGTGC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_134916.3  CG9663-RA (CG9663), mRNA 
0   0  4  13  54  NM_132027.2  CG15772-RA (CG15772), mRNA 
0   0  4  12  45  NM_080025.1  CG11387-RA, transcript variant A (ct), mRNA 
0   0  4  8  26  NM_132114.1  CG14442-RA (CG14442), mRNA 
0   0  4  5  9  NM_139548.2  CG12016-RA, transcript variant A (CG12016), mRNA 
0   0  4  5  9  NM_168022.1  CG12016-RB, transcript variant B (CG12016), mRNA 
0   0  3  7  6  NM_132035.1  CG4064-RA (CG4064), mRNA 
0   0  3  5  13  NM_167130.1  CG11387-RB, transcript variant B (ct), mRNA 
0   0  3  2  4  NM_206760.1  CG9214-RB, transcript variant B (Tob), mRNA 
0   0  3  2  4  NM_132876.3  CG9214-RA, transcript variant A (Tob), mRNA 
0   0  2  22  148  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
0   0  2  22  148  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
0   0  2  22  148  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
0   0  2  10  26  NM_079224.3  CG10037-RA (vvl), mRNA 
0   0  2  8  5  NM_057438.3  CG6172-RA, transcript variant A (vnd), mRNA 
0   0  2  8  5  NM_001042788.1  CG6172-RB, transcript variant B (vnd), mRNA 
0   0  2  6  8  NM_079861.2  CG1455-RA (CanA1), mRNA 
0   0  2  5  20  NM_206357.2  CG33261-RD, transcript variant D (Trl), mRNA 
0   0  2  5  6  NM_141171.2  CG11128-RC, transcript variant C (slif), mRNA 
0   0  2  5  4  NM_168977.1  CG11128-RA, transcript variant A (slif), mRNA 
0   0  2  5  4  NM_168978.1  CG11128-RB, transcript variant B (slif), mRNA 
0   0  2  4  18  NM_057502.2  CG1322-RB, transcript variant B (zfh1), mRNA 
0   0  2  4  18  NM_206356.2  CG33261-RB, transcript variant B (Trl), mRNA 
0   0  2  4  17  NM_170522.2  CG1322-RC, transcript variant C (zfh1), mRNA 
0   0  2  4  11  NM_135615.2  CG6700-RA (CG6700), mRNA 
0   0  2  4  5  NM_143026.1  CG6879-RA (CG6879), mRNA 
0   0  2  4  4  NM_170552.1  CG1499-RB, transcript variant B (CG1499), mRNA 
0   0  2  4  4  NM_143614.1  CG1499-RA, transcript variant A (CG1499), mRNA 
0   0  2  3  28  NM_206145.1  CG8963-RB, transcript variant B (CG8963), mRNA 
0   0  2  3  28  NM_137336.2  CG8963-RA, transcript variant A (CG8963), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
     

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