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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9579R-3 |
| Symbol |
AnnX |
Full Name |
Annexin X |
| CG No |
CG9579 |
Old CG No |
CG9579 |
| Synonyms |
CG9579, Annx, anxX, Ann, AnnX, annexin |
| Accession No (Link to NCBI) |
NM_057267.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
larval lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Fairchild MJ, Islam F, Tanentzapf G.
Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.
PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.
The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.
Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGAATACAAACCCGTGCCC |
| Primer Seq. 3' |
aatctagaggtaccGCGTGTCCAGTCCGTCACGTA |
| Predicted Fragment Size |
499 |
| IR fragment full Seq |
0001 ATGGAATACA AACCCGTGCC CACGGTTAAG GACGCAGCTC CCTTCGACGC CTCCCAGGAC
0061 GCCCAGGTGC TGCGGGCGGC GATGAAGGGA TTCGGCACCG ACGAGCAGGA AATCATCGAC
0121 GTGCTCGTCG GCAGNGAGCA ACCAGCAGAG GCAGACGATC AAGGCGGTTT ACGAAGCGGA
0181 GTTCGAGCGC GACCTGGTGG ACGATCTTAA GGACGAGCTG GGAGGCAAGT TCGAGGACGT
0241 GATCGTGGGT CTAATGATGC CACCAGTGGA GTACCTGTGC AAGCAANCTG CACGCCGCCA
0301 TGGCGGGCAG TCGGAACCGA GGAGGCCACG CTCGTCGAGA TCCTGTGCAC CAAGACCAAC
0361 GAGGAGATGG CCCAGATCGT GGCCGTCTAC GAGGAGCGCT ACCAGCgNCC GCTGGCCGAG
0421 CAGATGTGCA GCGAGACCTC CGGCTTTTTC CGCCGCNCTG CTCACGCTGA TCGTGACCGG
0481 AGTACGTGAC GGACTGGANC ANGc |
| in silico PCR Fragment |
0001 ATGGAATACA AACCCGTGCC CACGGTTAAG GACGCAGCTC CCTTCGACGC CTCCCAGGAC
0061 GCCCAGGTGC TGCGGGCGGC GATGAAGGGA TTCGGCACCG ACGAGCAGGA AATCATCGAC
0121 GTGCTCGTCG GCAG-GAGCA ACCAGCAGAG GCAGACGATC AAGGCGGTTT ACGAAGCGGA
0181 GTTCGAGCGC GACCTGGTGG ACGATCTTAA GGACGAGCTG GGAGGCAAGT TCGAGGACGT
0241 GATCGTGGGT CTAATGATGC CACCAGTGGA GTACCTGTGC AAGCAA-CTG CACGCCGCCA
0301 TGGCGGGCA- TCGGAACCGA GGAGGCCACG CTCGTCGAGA TCCTGTGCAC CAAGACCAAC
0361 GAGGAGATGG CCCAGATCGT GGCCGTCTAC GAGGAGCGCT ACCAGCGCCC GCTGGCCGAG
0421 CAGATGTGCA GCGAGACCTC CGGCTTTTTC CGCCGC-CTG CTCACGCTGA TCGTGACCGG
0481 AGTACGTGAC GGACTGGA-C ACGC |
| Assemble Data |
9579R-3.IR_full 1 ATGGAATACAAACCCGTGCCCACGGTTAAGGACGCAGCTCCCTTCGACGCCTCCCAGGAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9579R-3.in silico 1 ATGGAATACAAACCCGTGCCCACGGTTAAGGACGCAGCTCCCTTCGACGCCTCCCAGGAC 60
9579R-3.IR_full 61 GCCCAGGTGCTGCGGGCGGCGATGAAGGGATTCGGCACCGACGAGCAGGAAATCATCGAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9579R-3.in silico 61 GCCCAGGTGCTGCGGGCGGCGATGAAGGGATTCGGCACCGACGAGCAGGAAATCATCGAC 120
9579R-3.IR_full 121 GTGCTCGTCGGCAGNGAGCAACCAGCAGAGGCAGACGATCAAGGCGGTTTACGAAGCGGA 180
|||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
9579R-3.in silico 121 GTGCTCGTCGGCAG-GAGCAACCAGCAGAGGCAGACGATCAAGGCGGTTTACGAAGCGGA 180
9579R-3.IR_full 181 GTTCGAGCGCGACCTGGTGGACGATCTTAAGGACGAGCTGGGAGGCAAGTTCGAGGACGT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9579R-3.in silico 181 GTTCGAGCGCGACCTGGTGGACGATCTTAAGGACGAGCTGGGAGGCAAGTTCGAGGACGT 240
9579R-3.IR_full 241 GATCGTGGGTCTAATGATGCCACCAGTGGAGTACCTGTGCAAGCAANCTGCACGCCGCCA 300
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
9579R-3.in silico 241 GATCGTGGGTCTAATGATGCCACCAGTGGAGTACCTGTGCAAGCAA-CTGCACGCCGCCA 300
9579R-3.IR_full 301 TGGCGGGCAGTCGGAACCGAGGAGGCCACGCTCGTCGAGATCCTGTGCACCAAGACCAAC 360
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
9579R-3.in silico 301 TGGCGGGCA-TCGGAACCGAGGAGGCCACGCTCGTCGAGATCCTGTGCACCAAGACCAAC 360
9579R-3.IR_full 361 GAGGAGATGGCCCAGATCGTGGCCGTCTACGAGGAGCGCTACCAGCGNCCGCTGGCCGAG 420
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
9579R-3.in silico 361 GAGGAGATGGCCCAGATCGTGGCCGTCTACGAGGAGCGCTACCAGCGCCCGCTGGCCGAG 420
9579R-3.IR_full 421 CAGATGTGCAGCGAGACCTCCGGCTTTTTCCGCCGCNCTGCTCACGCTGATCGTGACCGG 480
|||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
9579R-3.in silico 421 CAGATGTGCAGCGAGACCTCCGGCTTTTTCCGCCGC-CTGCTCACGCTGATCGTGACCGG 480
9579R-3.IR_full 481 AGTACGTGACGGACTGGANCANGC 504
|||||||||||||||||| || ||
9579R-3.in silico 481 AGTACGTGACGGACTGGA-CACGC 504
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
481 |
0 |
0 |
7 |
NM_057267.3 |
CG9579-RA (AnnX), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_133132.1 |
CG14194-RA (CG14194), mRNA |
| 0 |
0 |
2 |
7 |
3 |
NM_132159.1 |
CG1677-RA (CG1677), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169828.1 |
CG31229-RA (CG31229), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167654.1 |
CG3400-RF, transcript variant F (Pfrx), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167651.1 |
CG3400-RA, transcript variant A (Pfrx), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167656.1 |
CG3400-RI, transcript variant I (Pfrx), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_058104.4 |
CG3400-RB, transcript variant B (Pfrx), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_058103.2 |
CG3400-RG, transcript variant G (Pfrx), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167652.1 |
CG3400-RD, transcript variant D (Pfrx), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167655.1 |
CG3400-RH, transcript variant H (Pfrx), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167653.1 |
CG3400-RE, transcript variant E (Pfrx), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169985.1 |
CG31465-RA (CG31465), mRNA |
| 0 |
0 |
1 |
17 |
32 |
NM_206531.1 |
CG5730-RC, transcript variant C (AnnIX), mRNA |
| 0 |
0 |
1 |
17 |
32 |
NM_057256.3 |
CG5730-RA, transcript variant A (AnnIX), mRNA |
| 0 |
0 |
1 |
17 |
32 |
NM_057255.2 |
CG5730-RB, transcript variant B (AnnIX), mRNA |
| 0 |
0 |
1 |
17 |
32 |
NM_206530.1 |
CG5730-RD, transcript variant D (AnnIX), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_137908.2 |
CG3085-RA (CG3085), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_169358.1 |
CG14685-RA, transcript variant A (Cap-H2), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141757.1 |
CG14685-RB, transcript variant B (Cap-H2), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_206471.1 |
CG14685-RD, transcript variant D (Cap-H2), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_169359.1 |
CG14685-RC, transcript variant C (Cap-H2), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137993.2 |
CG4735-RA (shu), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_078577.2 |
CG4147-RB, transcript variant B (Hsc70-3), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_167308.1 |
CG4147-RD, transcript variant D (Hsc70-3), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_167306.1 |
CG4147-RA, transcript variant A (Hsc70-3), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_167307.1 |
CG4147-RC, transcript variant C (Hsc70-3), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136597.3 |
CG8170-RA, transcript variant A (CG8170), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001043068.1 |
CG8170-RB, transcript variant B (CG8170), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078761.3 |
CG7234-RI (GluRIIB), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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