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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9578R-4 |
| Symbol |
CG9578 |
Full Name |
CG9578 |
| CG No |
CG9578 |
Old CG No |
CG9578 |
| Synonyms |
CG9578 |
| Accession No (Link to NCBI) |
NM_134533.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Parsons LM, Grzeschik NA, Amaratunga K, Burke P, Quinn LM, Richardson HE.
A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
G3 (Bethesda) (2017) 7(8) 2497-2509
[
PubMed ID = 28611255
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGACAGCGAGTTCATCCAGT |
| Primer Seq. 3' |
aatctagaggtaccGTGCAGCTCATCCTCGAACAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGGACAGCGA GTTCATCCAG TTCCACGACT GGGCCATCAA GTATGAGAAG TCGCACATCC
0061 TGAAGAGCAG CTGCCAACTG GGCACCGCCA AGTGCTGTCC CAAGGACTCG GCCGACCGCT
0121 GCGACCTCTG CCACTACCAG CACTCCCTGC AGCTGCCCCA CCTGCCCGAC ATGGTGTTCC
0181 ACAAGAATCG GCTGGTGTTA CAGCACANAG GACGGCGCCA CTCTCGAGTT CTGCCCCATG
0241 GACGCGCTGG CGCTGGTGGA CAATGGGAAN GCAGCCGCTG GAGGTCGCTC GTGCTCAGGA
0301 GTGGCGGGAA ACGCGTAACG AGCAGACAAT GGAGGAGAAG TTCAAGCCTT TCGACTGGAC
0361 ATTCACCTCC ACCTACCAGG GCACCATGAA CGAAAAAGTG CGATCGGAGA CCACGAATCA
0421 GACGCTGAAC AAGTTCAAGC TGATGCAGCG CGAGAACATC ATCTTCTACC ACGATTTAAN
0481 NCTGtTCGAG GATGAGCTGC AC |
| in silico PCR Fragment |
0001 TGGACAGCGA GTTCATCCAG TTCCACGACT GGGCCATCAA GTATGAGAAG TCGCACATCC
0061 TGAAGAGCAG CTGCCAACTG GGCACCGCCA AGTGCTGTCC CAAGGACTCG GCCGACCGCT
0121 GCGACCTCTG CCACTACCAG CACTCCCTGC AGCTGCCCCA CCTGCCCGAC ATGGTGTTCC
0181 ACAAGAATCG GCTGGTGTTA CAGCACA-AG GACGGCGCCA CTCTCGAGTT CTGCCCCATG
0241 GACGCGCTGG CGCTGGTGGA CAATGGGAA- GCAGCCGCTG GAGGTCGCTT GTGCTCAGGA
0301 GTGGCGGGAA ACGCGTAACG AGCAGACAAT GGAGGAGAAG TTCAAGCCTT TCGACTGGAC
0361 ATTCACCTCC ACCTACCAGG GCACCATGAA CGAAAAAGTG CGATCGGAGA CCACGAATCA
0421 GACGCTGAAC AAGTTCAAGC TGATGCAGCG CGAGAACATC ATCTTCTACC ACGATTTAAC
0481 TCTGTTCGAG GATGAGCTGC AC |
| Assemble Data |
9578R-4.IR_full 1 TGGACAGCGAGTTCATCCAGTTCCACGACTGGGCCATCAAGTATGAGAAGTCGCACATCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9578R-4.in silico 1 TGGACAGCGAGTTCATCCAGTTCCACGACTGGGCCATCAAGTATGAGAAGTCGCACATCC 60
9578R-4.IR_full 61 TGAAGAGCAGCTGCCAACTGGGCACCGCCAAGTGCTGTCCCAAGGACTCGGCCGACCGCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9578R-4.in silico 61 TGAAGAGCAGCTGCCAACTGGGCACCGCCAAGTGCTGTCCCAAGGACTCGGCCGACCGCT 120
9578R-4.IR_full 121 GCGACCTCTGCCACTACCAGCACTCCCTGCAGCTGCCCCACCTGCCCGACATGGTGTTCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9578R-4.in silico 121 GCGACCTCTGCCACTACCAGCACTCCCTGCAGCTGCCCCACCTGCCCGACATGGTGTTCC 180
9578R-4.IR_full 181 ACAAGAATCGGCTGGTGTTACAGCACANAGGACGGCGCCACTCTCGAGTTCTGCCCCATG 240
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
9578R-4.in silico 181 ACAAGAATCGGCTGGTGTTACAGCACA-AGGACGGCGCCACTCTCGAGTTCTGCCCCATG 240
9578R-4.IR_full 241 GACGCGCTGGCGCTGGTGGACAATGGGAANGCAGCCGCTGGAGGTCGCTCGTGCTCAGGA 300
||||||||||||||||||||||||||||| ||||||||||||||||||| ||||||||||
9578R-4.in silico 241 GACGCGCTGGCGCTGGTGGACAATGGGAA-GCAGCCGCTGGAGGTCGCTTGTGCTCAGGA 300
9578R-4.IR_full 301 GTGGCGGGAAACGCGTAACGAGCAGACAATGGAGGAGAAGTTCAAGCCTTTCGACTGGAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9578R-4.in silico 301 GTGGCGGGAAACGCGTAACGAGCAGACAATGGAGGAGAAGTTCAAGCCTTTCGACTGGAC 360
9578R-4.IR_full 361 ATTCACCTCCACCTACCAGGGCACCATGAACGAAAAAGTGCGATCGGAGACCACGAATCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9578R-4.in silico 361 ATTCACCTCCACCTACCAGGGCACCATGAACGAAAAAGTGCGATCGGAGACCACGAATCA 420
9578R-4.IR_full 421 GACGCTGAACAAGTTCAAGCTGATGCAGCGCGAGAACATCATCTTCTACCACGATTTAAN 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9578R-4.in silico 421 GACGCTGAACAAGTTCAAGCTGATGCAGCGCGAGAACATCATCTTCTACCACGATTTAAC 480
9578R-4.IR_full 481 CTGTTCGAGGATGAGCTGCAC 501
|||||||||||||||||||||
9578R-4.in silico 481 CTGTTCGAGGATGAGCTGCAC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_134533.2 |
CG9578-RA (CG9578), mRNA |
| 0.41 |
2 |
2 |
2 |
4 |
NM_135117.1 |
CG11034-RA (CG11034), mRNA |
| 0.2 |
1 |
2 |
2 |
3 |
NM_168897.1 |
CG32439-RA (CG32439), mRNA |
| 0 |
0 |
3 |
4 |
8 |
NM_132569.1 |
CG11245-RA (CG11245), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_141220.1 |
CG9783-RA (CG9783), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_169390.2 |
CG31374-RB, transcript variant B (CG31374), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_176460.1 |
CG31374-RC, transcript variant C (CG31374), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_169391.1 |
CG31374-RA, transcript variant A (CG31374), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_176123.1 |
CG33183-RA, transcript variant A (Hr46), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_176122.1 |
CG33183-RB, transcript variant B (Hr46), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_176121.1 |
CG33183-RC, transcript variant C (Hr46), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_142442.1 |
CG12320-RA (CG12320), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_134852.1 |
CG15395-RA (CG15395), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_140907.1 |
CG17732-RA (CG17732), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_143612.2 |
CG11333-RA (CG11333), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136598.2 |
CG8196-RA (Ance-4), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001038821.1 |
CG31732-RF, transcript variant F (yuri), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001038822.1 |
CG31732-RG, transcript variant G (yuri), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_141639.1 |
CG16779-RA (CG16779), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_001038820.1 |
CG31732-RE, transcript variant E (yuri), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_165106.4 |
CG31732-RB, transcript variant B (yuri), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_057719.3 |
CG8274-RA (Mtor), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_167410.2 |
CG14414-RB, transcript variant B (CG14414), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132736.2 |
CG14414-RA, transcript variant A (CG14414), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143303.1 |
CG6599-RA (CG6599), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_176729.1 |
CG14414-RC, transcript variant C (CG14414), mRNA |
| 0 |
0 |
0 |
9 |
6 |
NM_137881.1 |
CG3649-RA (CG3649), mRNA |
| 0 |
0 |
0 |
7 |
15 |
NM_141534.1 |
CG7443-RA (CG7443), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_143367.2 |
CG10011-RA (CG10011), mRNA |
| 0 |
0 |
0 |
6 |
19 |
NM_170430.2 |
CG31037-RA (ca), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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