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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9536R-2 
 Symbol CG9536  Full Name CG9536 
 CG No CG9536  Old CG No CG9536 
 Synonyms CG9536 
 Accession No (Link to NCBI) NM_135189.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAGCTCAACTGGCCAGAAACT 
 Primer Seq. 3' 
aatctagaggtaccGCGCGTCTTGAAGATCAGGTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CAGCTCAACT GGCCAGAAAC TGGCCGTACA TCTGGCAACA GCTCAATGCT CTGCTGCACA 
0061 ACACCTCGCC GGTCATAACG CTCATCTGCG TGGTGACCAC GTTCGGCTAC CTGCTATCCT 
0121 TCTCGGAGAC GGCCATCCTG CTGCTCAGCG TCACTCCCGG TTACATCCTG CCCAATGGCA 
0181 AGTTCTGGAT ATGGACCGCC TTCACGTTCT GCTTCATCGA GCTGCACTGG TGGGAGGTGG 
0241 CCGTCGACGT GGTCACCGTC GGCCTGTGCG GCAAGATGCT GGAACCGCTC TGGGGCCAGC 
0301 TGGAGATGTT CAAGTTCTTC GCGCTGAGCA ACTTTGGCGT CTCGCTGCTG ACCACTGTCT 
0361 ACTATTTGTT CTACTACATG GTCACCAAGA ACCCGACCAT TCTCTTCGAG GTGCACATTC 
0421 ACGGNCTGGC CGGATACGTG GCTGGCATCT GNGTAGCCGT ACGCCAGATA ATGCCCGATC 
0481 ACCTGATCTT CAATGACgcg C 
 in silico PCR Fragment 
0001 CAGCTCAACT GGCCAGAAAC TGGCCGTACA TCTGGCAACA GCTCAATGCT CTGCTGCACA 
0061 ACACCTCGCC GGTCATAACG CTCATCTGCG TGGTGACCAC GTTCGGCTAC CTGCTATCCT 
0121 TCTCGGAGAC GGCCATCCTG CTGCTCAGCG TCACTCCCGG TTACATCCTG CCCAATGGCA 
0181 AGTTCTGGAT ATGGACCGCC TTCACGTTCT GCTTCATCGA GCTGCACTGG TGGGAGGTGG 
0241 CCGTCGACGT GGTCACCGTC GGCCTGTGCG GCAAGATGCT GGAACCGCTC TGGGGCCAGC 
0301 TGGAGATGTT CAAGTTCTTC GCGCTGAGCA ACTTTGGCGT CTCGCTGCTG ACCACTGTCT 
0361 ACTATTTGTT CTACTACATG GTCACCAAGA ACCCGACCAT TCTCTTCGAG GTGCACATTC 
0421 ACGGCCTGGC CGGATACGTG GCTGGCATCT GCGTAGCCGT ACGCCAGATA ATGCCCGATC 
0481 ACCTGATCTT CAA-GACGCG C 
 Assemble Data 

9536R-2.IR_full       1   CAGCTCAACTGGCCAGAAACTGGCCGTACATCTGGCAACAGCTCAATGCTCTGCTGCACA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9536R-2.in silico     1   CAGCTCAACTGGCCAGAAACTGGCCGTACATCTGGCAACAGCTCAATGCTCTGCTGCACA 60


9536R-2.IR_full       61  ACACCTCGCCGGTCATAACGCTCATCTGCGTGGTGACCACGTTCGGCTACCTGCTATCCT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9536R-2.in silico     61  ACACCTCGCCGGTCATAACGCTCATCTGCGTGGTGACCACGTTCGGCTACCTGCTATCCT 120


9536R-2.IR_full       121 TCTCGGAGACGGCCATCCTGCTGCTCAGCGTCACTCCCGGTTACATCCTGCCCAATGGCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9536R-2.in silico     121 TCTCGGAGACGGCCATCCTGCTGCTCAGCGTCACTCCCGGTTACATCCTGCCCAATGGCA 180


9536R-2.IR_full       181 AGTTCTGGATATGGACCGCCTTCACGTTCTGCTTCATCGAGCTGCACTGGTGGGAGGTGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9536R-2.in silico     181 AGTTCTGGATATGGACCGCCTTCACGTTCTGCTTCATCGAGCTGCACTGGTGGGAGGTGG 240


9536R-2.IR_full       241 CCGTCGACGTGGTCACCGTCGGCCTGTGCGGCAAGATGCTGGAACCGCTCTGGGGCCAGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9536R-2.in silico     241 CCGTCGACGTGGTCACCGTCGGCCTGTGCGGCAAGATGCTGGAACCGCTCTGGGGCCAGC 300


9536R-2.IR_full       301 TGGAGATGTTCAAGTTCTTCGCGCTGAGCAACTTTGGCGTCTCGCTGCTGACCACTGTCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9536R-2.in silico     301 TGGAGATGTTCAAGTTCTTCGCGCTGAGCAACTTTGGCGTCTCGCTGCTGACCACTGTCT 360


9536R-2.IR_full       361 ACTATTTGTTCTACTACATGGTCACCAAGAACCCGACCATTCTCTTCGAGGTGCACATTC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9536R-2.in silico     361 ACTATTTGTTCTACTACATGGTCACCAAGAACCCGACCATTCTCTTCGAGGTGCACATTC 420


9536R-2.IR_full       421 ACGGNCTGGCCGGATACGTGGCTGGCATCTGNGTAGCCGTACGCCAGATAATGCCCGATC 480
                          |||| |||||||||||||||||||||||||| ||||||||||||||||||||||||||||
9536R-2.in silico     421 ACGGCCTGGCCGGATACGTGGCTGGCATCTGCGTAGCCGTACGCCAGATAATGCCCGATC 480


9536R-2.IR_full       481 ACCTGATCTTCAATGACGCGC 501
                          ||||||||||||| |||||||
9536R-2.in silico     481 ACCTGATCTTCAA-GACGCGC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_135189.2  CG9536-RA (CG9536), mRNA 
0.62   3  2  5  6  NM_142315.2  CG8977-RA, transcript variant A (Cctgamma), mRNA 
0.62   3  2  5  6  NM_169727.1  CG8977-RB, transcript variant B (Cctgamma), mRNA 
0.2   1  2  4  5  NM_170004.1  CG7070-RB, transcript variant B (PyK), mRNA 
0.2   1  2  4  5  NM_079724.2  CG7070-RA, transcript variant A (PyK), mRNA 
0.2   1  2  2  3  NM_134869.2  CG2964-RA (CG2964), mRNA 
0   0  3  2  3  NM_168051.2  CG32258-RA, transcript variant A (Gr64e), mRNA 
0   0  3  2  3  NM_001031935.1  CG32258-RB, transcript variant B (Gr64e), mRNA 
0   0  3  2  3  NM_168050.2  CG14987-RA, transcript variant A (Gr64d), mRNA 
0   0  3  2  3  NM_140768.1  CG14353-RA (CG14353), mRNA 
0   0  2  5  4  NM_136636.3  CG13739-RA (CG13739), mRNA 
0   0  2  4  3  NM_167441.2  CG9176-RB, transcript variant B (cngl), mRNA 
0   0  2  4  3  NM_078608.3  CG9176-RC, transcript variant C (cngl), mRNA 
0   0  2  3  4  NM_139849.2  CG8598-RA, transcript variant A (eco), mRNA 
0   0  2  3  4  NM_168201.2  CG8598-RB, transcript variant B (eco), mRNA 
0   0  2  3  3  NM_168196.1  CG32387-RA, transcript variant A (CG32387), mRNA 
0   0  2  3  3  NM_001043123.1  CG32387-RC, transcript variant C (CG32387), mRNA 
0   0  2  3  3  NM_168195.1  CG32387-RB, transcript variant B (CG32387), mRNA 
0   0  1  5  5  NM_168040.1  CG32266-RA (CG32266), mRNA 
0   0  1  4  8  NM_143754.2  CG18660-RC, transcript variant C (Nckx30C), mRNA 
0   0  1  4  8  NM_164870.1  CG18660-RB, transcript variant B (Nckx30C), mRNA 
0   0  1  4  8  NM_164869.1  CG18660-RA, transcript variant A (Nckx30C), mRNA 
0   0  1  3  7  NM_135646.2  CG6230-RA (CG6230), mRNA 
0   0  1  2  7  NM_143320.2  CG18437-RA (CG18437), mRNA 
0   0  1  2  5  NM_140618.1  CG13040-RA (CG13040), mRNA 
0   0  1  2  5  NM_136854.1  CG13194-RA (pyr), mRNA 
0   0  1  2  3  NM_057817.2  CG10637-RA, transcript variant A (Nak), mRNA 
0   0  1  2  3  NM_057818.2  CG10637-RB, transcript variant B (Nak), mRNA 
0   0  1  2  2  NM_057270.3  CG7123-RA, transcript variant A (LanB1), mRNA 
0   0  1  2  2  NM_141887.1  CG3532-RA (CG3532), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+- 
DC
+ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
++ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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