NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9511R-1

Symbol CG9511 Full Name CG9511

CG No CG9511 Old CG No CG9511

Synonyms CT26922, CG9511

Accession No (Link to NCBI) NM_135180.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCCCAGCTGGATCTTGGTGTG

Primer Seq. 3'

aatctagaggtaccAGCTGCGCCCCGAACTCATTG

Predicted Fragment Size

IR fragment full Seq

0001 CCCCAGCTGG ATCTTGGTGT GAAGGACTTC CAGCTGGAGC TTCTCGCGCG AGTTAAGCGA 
0061 CCTGGTGCAA CAGCGGATTG AACCGAGTCT CAACGACTAC CTGGATCGTC TGGGACGCAT 
0121 TCCTCGGAAT GCCAACTATA CAGAAGAGAT GAGTNGCAGC TCGCGCAACA CCAAAGCTTC 
0181 AATTGAAGGT GACGCTCATC ANAGCAGCTG CTNCGNACGT GCGTCCGCAC CTGGACACNG 
0241 GATATGGAGT ACACCATTGT GCTGCGCAAC CTCGTCTTTC TGAACCGAGT CAGATCCACG 
0301 CTGAGGGCTG CCGAGGAGAC TAGCACACAA GAGATCCGAA TGATGCGCAT GCACCGCCTC 
0361 TGCCAGCAAC TTGACCGACC GGCTCCTCAT NCGCGGCAGT CGCCTGATCA ACGAACAGAC 
0421 AGTCGGGGCG GCATTGGGGC AACTCAATCT GACCAAAAAG GAGGTGAACT CCACCGGCTT 
0481 GGATCTCAAT GAGTTCgGGG NGCAGCT

in silico PCR Fragment

0001 CCCCAGCTGG ATCTTGGTGT GAAGGACTTC CAGCTGGAGC TTCT-GCGCG AGTTAAGCGA 
0061 CCTGGTGCAA CAGCGGATTG AACCGAGTCT CAACGACTAC CTGGATCGTC TAGGACGCAT 
0121 TCCTCGGAAT GCCAACTACA CCGAAGAGAT GAGT-GCAGC TCGCGCAACA CCAAAGCTTC 
0181 AATTGAAGGT GACGCTCATC A-AGCAGCTG CT-CG-ACGT GCGTCCGCAC CTGGACAC-G 
0241 GATATGGAGT ACACCATTGT GCTGCGCAAC CTGGTCTTTC TGAACCGAGT CAGATCCACG 
0301 CTGAGGGCTG CCGAGGAGAC TAGCACACAA GAGATCCGAA TGATGCGCAT GCACCGCCTC 
0361 TGCCAGCAAC TTGACCGACC GGCTCCTCAT -CGCGGCAGT CGCCTGATCA ACGAACAGAC 
0421 AGTCGGGGCG GCATTGGGGC AACTCAATCT GACCAAAAAG GAGGTGAACT CCACCGGCTT 
0481 GGATCTCAAT GAGTTCGGGG CGCAGCT

Assemble Data


9511R-1.IR_full       1   CCCCAGCTGGATCTTGGTGTGAAGGACTTCCAGCTGGAGCTTCTCGCGCGAGTTAAGCGA 60
                          |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
9511R-1.in silico     1   CCCCAGCTGGATCTTGGTGTGAAGGACTTCCAGCTGGAGCTTCT-GCGCGAGTTAAGCGA 60


9511R-1.IR_full       61  CCTGGTGCAACAGCGGATTGAACCGAGTCTCAACGACTACCTGGATCGTCTGGGACGCAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
9511R-1.in silico     61  CCTGGTGCAACAGCGGATTGAACCGAGTCTCAACGACTACCTGGATCGTCTAGGACGCAT 120


9511R-1.IR_full       121 TCCTCGGAATGCCAACTATACAGAAGAGATGAGTNGCAGCTCGCGCAACACCAAAGCTTC 180
                          |||||||||||||||||| || |||||||||||| |||||||||||||||||||||||||
9511R-1.in silico     121 TCCTCGGAATGCCAACTACACCGAAGAGATGAGT-GCAGCTCGCGCAACACCAAAGCTTC 180


9511R-1.IR_full       181 AATTGAAGGTGACGCTCATCANAGCAGCTGCTNCGNACGTGCGTCCGCACCTGGACACNG 240
                          ||||||||||||||||||||| |||||||||| || |||||||||||||||||||||| |
9511R-1.in silico     181 AATTGAAGGTGACGCTCATCA-AGCAGCTGCT-CG-ACGTGCGTCCGCACCTGGACAC-G 240


9511R-1.IR_full       241 GATATGGAGTACACCATTGTGCTGCGCAACCTCGTCTTTCTGAACCGAGTCAGATCCACG 300
                          |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
9511R-1.in silico     241 GATATGGAGTACACCATTGTGCTGCGCAACCTGGTCTTTCTGAACCGAGTCAGATCCACG 300


9511R-1.IR_full       301 CTGAGGGCTGCCGAGGAGACTAGCACACAAGAGATCCGAATGATGCGCATGCACCGCCTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9511R-1.in silico     301 CTGAGGGCTGCCGAGGAGACTAGCACACAAGAGATCCGAATGATGCGCATGCACCGCCTC 360


9511R-1.IR_full       361 TGCCAGCAACTTGACCGACCGGCTCCTCATNCGCGGCAGTCGCCTGATCAACGAACAGAC 420
                          |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
9511R-1.in silico     361 TGCCAGCAACTTGACCGACCGGCTCCTCAT-CGCGGCAGTCGCCTGATCAACGAACAGAC 420


9511R-1.IR_full       421 AGTCGGGGCGGCATTGGGGCAACTCAATCTGACCAAAAAGGAGGTGAACTCCACCGGCTT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9511R-1.in silico     421 AGTCGGGGCGGCATTGGGGCAACTCAATCTGACCAAAAAGGAGGTGAACTCCACCGGCTT 480


9511R-1.IR_full       481 GGATCTCAATGAGTTCGGGGNGCAGCT 507
                          |||||||||||||||||||| ||||||
9511R-1.in silico     481 GGATCTCAATGAGTTCGGGGCGCAGCT 507

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	6	NM_135180.1	CG9511-RA (CG9511), mRNA
0.2	1	4	2	3	NM_136888.2	CG12367-RA (CG12367), mRNA
0	0	3	5	2	NM_137662.1	CG11136-RA (CG11136), mRNA
0	0	3	2	2	NM_137061.2	CG6337-RA (CG6337), mRNA
0	0	2	5	4	NM_057412.3	CG10223-RA (Top2), mRNA
0	0	2	4	3	NM_165551.2	CG30496-RA (CG30496), mRNA
0	0	2	3	3	NM_143237.2	CG5480-RA (CG5480), mRNA
0	0	2	2	2	NM_132914.2	CG9723-RA (CG9723), mRNA
0	0	1	8	3	NM_135804.2	CG7099-RA (CG7099), mRNA
0	0	1	4	3	NM_078749.3	CG2937-RA (mRpS2), mRNA
0	0	1	4	3	NM_058033.3	CG7581-RA (Bub3), mRNA
0	0	1	3	6	NM_176339.1	CG9674-RD, transcript variant D (CG9674), mRNA
0	0	1	3	6	NM_140665.1	CG9674-RA, transcript variant A (CG9674), mRNA
0	0	1	3	4	NM_142427.2	CG7940-RA (CG7940), mRNA
0	0	1	3	3	NM_168686.1	CG9674-RB, transcript variant B (CG9674), mRNA
0	0	1	3	3	NM_168687.1	CG9674-RC, transcript variant C (CG9674), mRNA
0	0	1	3	3	NM_141594.1	CG11970-RA (CG11970), mRNA
0	0	1	3	2	NM_206290.1	CG33279-RA (CG33279), mRNA
0	0	1	2	10	NM_143745.2	CG18389-RA (Eip93F), mRNA
0	0	1	2	3	NM_001038863.1	CG33960-RA (CG33960), mRNA
0	0	1	2	2	NM_169726.1	CG6588-RA, transcript variant A (Fas1), mRNA
0	0	1	2	2	NM_169724.1	CG6588-RC, transcript variant C (Fas1), mRNA
0	0	1	2	2	NM_169725.1	CG6588-RB, transcript variant B (Fas1), mRNA
0	0	0	6	5	NM_136986.2	CG3884-RB, transcript variant B (CG3884), mRNA
0	0	0	5	12	NM_080345.2	CG1697-RB, transcript variant B (rho-4), mRNA
0	0	0	5	12	NM_167291.2	CG1697-RC, transcript variant C (rho-4), mRNA
0	0	0	5	12	NM_167290.2	CG1697-RA, transcript variant A (rho-4), mRNA
0	0	0	5	8	NM_166125.2	CG18255-RA, transcript variant A (Strn-Mlck), mRNA
0	0	0	5	7	NM_057957.4	CG3572-RB, transcript variant B (vimar), mRNA
0	0	0	5	7	NM_206045.1	CG3572-RC, transcript variant C (vimar), mRNA

Back