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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9481R-1 |
| Symbol |
Ugt37b1 |
Full Name |
UDP-glycosyltransferase 37b1 |
| CG No |
CG9481 |
Old CG No |
CG9481 |
| Synonyms |
AC 005285A, DmeUgt37b1, CG9481, Ugt37b1 |
| Accession No (Link to NCBI) |
NM_080269.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGGTGTCTCGCCCTTCAGATA |
| Primer Seq. 3' |
aatctagaggtaccCCCAAAAATGATGGCGGGTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGGTGTCTCG CCCTTCAGAT AGTGGTTCTG ATTGCCGGAG CCCATGGAGC CAACATTCTG
0061 GGTCTGTTCA CCAGCCTGAG TCCGTCGCAC TTGGTCATCC AGATGTCGAT GGCCCGGATT
0121 CTGGCCGAGA GGGGGCACAA CGTGACTGTA GTTACGATCC TAAAACCTCC GTCTCTACAC
0181 AAGGATATCA ACCACATTCT GGTGCCAATG GAAGAGGATA TCCTACAGGC ATTCAATTCT
0241 GTGGTCGGTG GAATGACAAA GACGGACAAT AGCAATGCAT ACGTGTCCAT GTTTAGATCG
0301 GTCAGACAGT TGAGCGAGAC GTTCTCCAAG ATGGGTGATG TGATGAAGCA AcCGTTGGTT
0361 AAAGATNTCT ACGAGCACCC GGACAACAAG TTCGATTTGG TCATGGTGGG CTACTTTATG
0421 AACTGTTATC AACTgGCCCT GGCCCACAAA CTGAAGGTTN CGCTGGTGGT GGCCCTTTCG
0481 AACCCGCCAT CATTTTTGGG |
| in silico PCR Fragment |
0001 AGGTGTCTCG CCCTTCAGAT AGTGGTTCTG ATTGCCGGAG CCCATGGAGC CAACATTCTG
0061 GGTCTGTTCA CCAGCCTGAG TCCGTCGCAC TTGGTCATCC AGATGTCGAT GGCCCGGATT
0121 CTGGCCGAGA GGGGGCACAA CGTGACTGTA GTTACGATCC TAAAACCTCC GTCTCTACAC
0181 AAGGATATCA ACCACATTCT GGTGCCAATG GAAGAGGATA TCCTACAGGC ATTCAATTCT
0241 GTGGTCGGTG GAATGACAAA GACGGACAAT AGCAATGCAT ACGTGTCCAT GTTTAGATCG
0301 GTCAGACAGT TGAGCGAGAC GTTCTCCAAG ATGGGTGATG TGATGAAGCA ACCGTTGGTT
0361 AAAGATCTCT ACGAGCACCC GGACAACAAG TTCGATTTGG TCATGGTGGG CTACTTTATG
0421 AACTGTTATC AACTGGCCCT GGCCCACAAA CTGAAGGTTC CGCTGGTGGT GGCCCTTTCG
0481 AACCCGCCAT CATTTTTGGG |
| Assemble Data |
9481R-1.IR_full 1 AGGTGTCTCGCCCTTCAGATAGTGGTTCTGATTGCCGGAGCCCATGGAGCCAACATTCTG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9481R-1.in silico 1 AGGTGTCTCGCCCTTCAGATAGTGGTTCTGATTGCCGGAGCCCATGGAGCCAACATTCTG 60
9481R-1.IR_full 61 GGTCTGTTCACCAGCCTGAGTCCGTCGCACTTGGTCATCCAGATGTCGATGGCCCGGATT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9481R-1.in silico 61 GGTCTGTTCACCAGCCTGAGTCCGTCGCACTTGGTCATCCAGATGTCGATGGCCCGGATT 120
9481R-1.IR_full 121 CTGGCCGAGAGGGGGCACAACGTGACTGTAGTTACGATCCTAAAACCTCCGTCTCTACAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9481R-1.in silico 121 CTGGCCGAGAGGGGGCACAACGTGACTGTAGTTACGATCCTAAAACCTCCGTCTCTACAC 180
9481R-1.IR_full 181 AAGGATATCAACCACATTCTGGTGCCAATGGAAGAGGATATCCTACAGGCATTCAATTCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9481R-1.in silico 181 AAGGATATCAACCACATTCTGGTGCCAATGGAAGAGGATATCCTACAGGCATTCAATTCT 240
9481R-1.IR_full 241 GTGGTCGGTGGAATGACAAAGACGGACAATAGCAATGCATACGTGTCCATGTTTAGATCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9481R-1.in silico 241 GTGGTCGGTGGAATGACAAAGACGGACAATAGCAATGCATACGTGTCCATGTTTAGATCG 300
9481R-1.IR_full 301 GTCAGACAGTTGAGCGAGACGTTCTCCAAGATGGGTGATGTGATGAAGCAACCGTTGGTT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9481R-1.in silico 301 GTCAGACAGTTGAGCGAGACGTTCTCCAAGATGGGTGATGTGATGAAGCAACCGTTGGTT 360
9481R-1.IR_full 361 AAAGATNTCTACGAGCACCCGGACAACAAGTTCGATTTGGTCATGGTGGGCTACTTTATG 420
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
9481R-1.in silico 361 AAAGATCTCTACGAGCACCCGGACAACAAGTTCGATTTGGTCATGGTGGGCTACTTTATG 420
9481R-1.IR_full 421 AACTGTTATCAACTGGCCCTGGCCCACAAACTGAAGGTTNCGCTGGTGGTGGCCCTTTCG 480
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
9481R-1.in silico 421 AACTGTTATCAACTGGCCCTGGCCCACAAACTGAAGGTTCCGCTGGTGGTGGCCCTTTCG 480
9481R-1.IR_full 481 AACCCGCCATCATTTTTGGG 500
||||||||||||||||||||
9481R-1.in silico 481 AACCCGCCATCATTTTTGGG 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_080269.2 |
CG9481-RA (Ugt37b1), mRNA |
| 0 |
0 |
4 |
4 |
6 |
NM_169222.2 |
CG31462-RA (CG31462), mRNA |
| 0 |
0 |
3 |
19 |
22 |
NM_141972.1 |
CG5999-RA (CG5999), mRNA |
| 0 |
0 |
3 |
10 |
17 |
NM_165193.1 |
CG17932-RB, transcript variant B (Ugt36Bc), mRNA |
| 0 |
0 |
3 |
10 |
17 |
NM_144370.2 |
CG17932-RA, transcript variant A (Ugt36Bc), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_136599.2 |
CG8193-RA (CG8193), mRNA |
| 0 |
0 |
1 |
11 |
5 |
NM_132061.1 |
CG5937-RA (CG5937), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_079342.2 |
CG5893-RA (D), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137367.2 |
CG18467-RA (CG18467), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_142205.2 |
CG6130-RA (CG6130), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168920.1 |
CG32442-RA, transcript variant A (CG32442), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168921.1 |
CG32442-RB, transcript variant B (CG32442), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079349.2 |
CG5185-RA (Tom), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132613.2 |
CG4645-RA (CG4645), mRNA |
| 0 |
0 |
0 |
16 |
37 |
NM_141971.1 |
CG5724-RA (CG5724), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_001043114.1 |
CG33484-RD, transcript variant D (zormin), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_001043112.1 |
CG33484-RB, transcript variant B (zormin), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_206240.1 |
CG33484-RA, transcript variant A (zormin), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_141633.1 |
CG8135-RA (CG8135), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_078723.2 |
CG3022-RA, transcript variant A (GABA-B-R3), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_164387.1 |
CG3022-RB, transcript variant B (GABA-B-R3), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_057638.3 |
CG14472-RA (poe), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_001015357.1 |
CG40390-PA.3 (CG40390), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_141606.2 |
CG11990-RA (hyx), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_131932.2 |
CG2982-RB, transcript variant B (CG2982), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_167007.1 |
CG2982-RA, transcript variant A (CG2982), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079282.1 |
CG3322-RA (LanB2), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142395.1 |
CG7431-RA (CG7431), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_166653.1 |
CG3182-RB, transcript variant B (sei), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
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