NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9427R-2

Symbol CG9427 Full Name CG9427

CG No CG9427 Old CG No CG9427

Synonyms CG9427

Accession No (Link to NCBI) NM_141662.2

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCTGAGTGCAAGGGCAATCG

Primer Seq. 3'

aatctagaggtaccCTGGCAGATTGTGCTGCTGCT

Predicted Fragment Size

IR fragment full Seq

0001 GGCTGAGTGC AAGGGCAATC GCCTCCAGAG TTGCGTCATC AACAGCACCG CAAACCAGGC 
0061 GGCGCAGGTT AAATTCGTGG TGTGCCAGAT GCTAGCTCCG GATTATTCCC GCATTGATCA 
0121 GTGTGCCAAT GAGGcAGGCC TGCTCACCGA TGTGGTCCAC TGCCTGTCCA GCGAGACGGG 
0181 CACCAAATTG CAGCTGCAGG CGGAGCTGGN TGACCAAACA GTACAGCCCG AGCTTCATTC 
0241 CCACCATCGT CTACAATGGC GTCTTCGATC AGCAGCTGCA GGANCCATTC GCTGCGcGAT 
0301 TTCCGCGGCA CGGTTTGTTA TATGTTGCGT CAACAAAANC TTGCTGCCCA GCAGCAGCAC 
0361 AATCTGCCAG

in silico PCR Fragment

0001 GGCTGAGTGC AAGGGCAATC GCCTCCAGAG TTGCGTCATC AACAGCACCG CAAACCAGGC 
0061 GGCGCAGGTT AAATTCGTGG TGTGCCAGAT GCTAGCTCCG GATTATTCCC GCATTGATCA 
0121 GTGTGCCAAT GAGGCAGGCC TGCTCACCGA TGTGGTCCAC TGCCTGTCCA GCGAGACGGG 
0181 CACCAAATTG CAGCTGCAGG CGGAGCTGG- TGACCAAACA GTACAGCCCG AGCTTCATTC 
0241 CCACCATCGT CTACAATGGC GTCTTCGATC AGCAGCTGCA GGA-CCATTC GCTGCGCGAT 
0301 TTCCGCGGCA CGGTTTGTTA TATGTTGCGT CAACAAAA-C TTGCTGCCCA GCAGCAGCAC 
0361 AATCTGCCAG

Assemble Data


9427R-2.IR_full       1   GGCTGAGTGCAAGGGCAATCGCCTCCAGAGTTGCGTCATCAACAGCACCGCAAACCAGGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9427R-2.in silico     1   GGCTGAGTGCAAGGGCAATCGCCTCCAGAGTTGCGTCATCAACAGCACCGCAAACCAGGC 60


9427R-2.IR_full       61  GGCGCAGGTTAAATTCGTGGTGTGCCAGATGCTAGCTCCGGATTATTCCCGCATTGATCA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9427R-2.in silico     61  GGCGCAGGTTAAATTCGTGGTGTGCCAGATGCTAGCTCCGGATTATTCCCGCATTGATCA 120


9427R-2.IR_full       121 GTGTGCCAATGAGGCAGGCCTGCTCACCGATGTGGTCCACTGCCTGTCCAGCGAGACGGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9427R-2.in silico     121 GTGTGCCAATGAGGCAGGCCTGCTCACCGATGTGGTCCACTGCCTGTCCAGCGAGACGGG 180


9427R-2.IR_full       181 CACCAAATTGCAGCTGCAGGCGGAGCTGGNTGACCAAACAGTACAGCCCGAGCTTCATTC 240
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
9427R-2.in silico     181 CACCAAATTGCAGCTGCAGGCGGAGCTGG-TGACCAAACAGTACAGCCCGAGCTTCATTC 240


9427R-2.IR_full       241 CCACCATCGTCTACAATGGCGTCTTCGATCAGCAGCTGCAGGANCCATTCGCTGCGCGAT 300
                          ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
9427R-2.in silico     241 CCACCATCGTCTACAATGGCGTCTTCGATCAGCAGCTGCAGGA-CCATTCGCTGCGCGAT 300


9427R-2.IR_full       301 TTCCGCGGCACGGTTTGTTATATGTTGCGTCAACAAAANCTTGCTGCCCAGCAGCAGCAC 360
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
9427R-2.in silico     301 TTCCGCGGCACGGTTTGTTATATGTTGCGTCAACAAAA-CTTGCTGCCCAGCAGCAGCAC 360


9427R-2.IR_full       361 AATCTGCCAG 370
                          ||||||||||
9427R-2.in silico     361 AATCTGCCAG 370

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	349	0	0	0	NM_141662.2	CG9427-RA (CG9427), mRNA
0.57	2	2	3	3	NM_137701.2	CG4030-RA (CG4030), mRNA
0.28	1	3	6	12	NM_079524.3	CG1030-RA, transcript variant A (Scr), mRNA
0.28	1	3	6	12	NM_206443.1	CG1030-RB, transcript variant B (Scr), mRNA
0.28	1	3	6	12	NM_206442.1	CG1030-RC, transcript variant C (Scr), mRNA
0.28	1	2	2	3	NM_130500.2	CG5254-RA (CG5254), mRNA
0.28	1	2	2	3	NM_136156.1	CG10463-RA (CG10463), mRNA
0	0	3	4	3	NM_140688.2	CG7728-RA (CG7728), mRNA
0	0	3	3	4	NM_167504.1	CG12223-RB, transcript variant B (Dsp1), mRNA
0	0	3	3	4	NM_167502.2	CG12223-RD, transcript variant D (Dsp1), mRNA
0	0	3	3	4	NM_080715.3	CG12223-RC, transcript variant C (Dsp1), mRNA
0	0	3	3	4	NM_206762.1	CG12223-RE, transcript variant E (Dsp1), mRNA
0	0	3	3	4	NM_167503.2	CG12223-RA, transcript variant A (Dsp1), mRNA
0	0	2	4	4	NM_135255.1	CG13783-RA (CG13783), mRNA
0	0	2	3	12	NM_132584.2	CG32654-RC (CG32654), mRNA
0	0	2	3	6	NM_140901.2	CG7762-RA (Rpn1), mRNA
0	0	2	2	4	NM_079641.2	CG4938-RA (Aats-ser), mRNA
0	0	1	6	2	NM_164823.2	CG17608-RB, transcript variant B (fu12), mRNA
0	0	1	6	2	NM_135387.2	CG17608-RA, transcript variant A (fu12), mRNA
0	0	1	5	5	NM_167520.2	CG32575-RB, transcript variant B (hang), mRNA
0	0	1	5	5	NM_167521.2	CG32575-RA, transcript variant A (hang), mRNA
0	0	1	5	2	NM_140250.1	CG5964-RA (CG5964), mRNA
0	0	1	4	20	NM_176591.1	CG15532-RC, transcript variant C (hdc), mRNA
0	0	1	4	20	NM_079853.2	CG15532-RA, transcript variant A (hdc), mRNA
0	0	1	4	15	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
0	0	1	4	15	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
0	0	1	4	8	NM_206299.1	CG7391-RC, transcript variant C (Clk), mRNA
0	0	1	4	8	NM_079240.2	CG7391-RA, transcript variant A (Clk), mRNA
0	0	1	4	8	NM_001014574.1	CG7391-RF, transcript variant F (Clk), mRNA
0	0	1	4	8	NM_001014575.1	CG7391-RE, transcript variant E (Clk), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance
+

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