NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9415R-1

Symbol Xbp1 Full Name X box binding protein-1

CG No CG9415 Old CG No CG9415

Synonyms xbp1, CG9415, clone 1.41, 138/3, l(2)k13803, anon-EST:Liang-1.41, Xbp1, XBP-1

Accession No (Link to NCBI) NM_079983.2

Inserted Chr. ll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCTAATTACCGTGCCGCGGAC

Primer Seq. 3'

aatctagaggtaccTGGCGTTGGATTGGCTGATGC

Predicted Fragment Size

IR fragment full Seq

0001 GCTAATTACC GTGCCGCGGA CAGcAATCAC ATCGAATAAT CTGCCCAAAT TGCGGCCCGc 
0061 CCCCCTTACG GCAGCTCTgN TGAAGGTGTC GGCCACGcCC TCCGCCTCGC CCACGCCCTC 
0121 GAGTTCGGGA TACGCATCCT CGTCGAACAT GGATGACGAT AACATGGCTG CATCCCAGCC 
0181 CAAGGCCAAG AAGCGTCGCC TGGACCATCT AACCTGGGAG GAGAAAGTGC AAAGAAAGAA 
0241 GCTAAAGAAC CGTGTGGCCG CCCAGACATC GCGTGACCGC AAGAAGGCAC GCATGGAGGA 
0301 GATGGACTAC GAGATCAAGG AGCTGACAGA CAGAACGGAA ATACTACAGA ACAAGTGCGA 
0361 TAGNCTGCAG GCCATCAACG AGTCACTGCT GGCCAAGAAC CACAAGCTGG ACTCGGAGCT 
0421 GGAGCTGCTG CGCCAAGAAC TCGCCGAACT GAAGCAGCAA CAGCAGCACA ACACCAGATG 
0481 CATCAGCCAA TCCAACGCCA

in silico PCR Fragment

0001 GCTAATTACC GTGCCGCGGA CAGCAATCAC ATCGAATAAT CTGCCCAAAT TGCGGCCCGC 
0061 CCCCCTTACG GCAGCTCTGC TGAAGGTGTC GGCCACGCCC TCCGCCTCGC CCACGCCCTC 
0121 GAGTTCGGGA TACGCATCCT CGTCGAACAT GGATGACGAT AACATGGCTG CATCCCAGCC 
0181 CAAGGCCAAG AAGCGTCGCC TGGACCATCT AACCTGGGAG GAGAAAGTGC AAAGAAAGAA 
0241 GCTAAAGAAC CGTGTGGCCG CCCAGACATC GCGTGACCGC AAGAAGGCAC GCATGGAGGA 
0301 GATGGACTAC GAGATCAAGG AGCTGACAGA CAGAACGGAA ATACTACAGA ACAAGTGCGA 
0361 TAGCCTGCAG GCCATCAACG AGTCACTGCT GGCCAAGAAC CACAAGCTGG ACTCGGAGCT 
0421 GGAGCTGCTG CGCCAAGAAC TCGCCGAACT GAAGCAGCAA CAGCAGCACA ACACCAGATG 
0481 CATCAGCCAA TCCAACGCCA

Assemble Data


9415R-1.IR_full       1   GCTAATTACCGTGCCGCGGACAGCAATCACATCGAATAATCTGCCCAAATTGCGGCCCGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9415R-1.in silico     1   GCTAATTACCGTGCCGCGGACAGCAATCACATCGAATAATCTGCCCAAATTGCGGCCCGC 60


9415R-1.IR_full       61  CCCCCTTACGGCAGCTCTGNTGAAGGTGTCGGCCACGCCCTCCGCCTCGCCCACGCCCTC 120
                          ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
9415R-1.in silico     61  CCCCCTTACGGCAGCTCTGCTGAAGGTGTCGGCCACGCCCTCCGCCTCGCCCACGCCCTC 120


9415R-1.IR_full       121 GAGTTCGGGATACGCATCCTCGTCGAACATGGATGACGATAACATGGCTGCATCCCAGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9415R-1.in silico     121 GAGTTCGGGATACGCATCCTCGTCGAACATGGATGACGATAACATGGCTGCATCCCAGCC 180


9415R-1.IR_full       181 CAAGGCCAAGAAGCGTCGCCTGGACCATCTAACCTGGGAGGAGAAAGTGCAAAGAAAGAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9415R-1.in silico     181 CAAGGCCAAGAAGCGTCGCCTGGACCATCTAACCTGGGAGGAGAAAGTGCAAAGAAAGAA 240


9415R-1.IR_full       241 GCTAAAGAACCGTGTGGCCGCCCAGACATCGCGTGACCGCAAGAAGGCACGCATGGAGGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9415R-1.in silico     241 GCTAAAGAACCGTGTGGCCGCCCAGACATCGCGTGACCGCAAGAAGGCACGCATGGAGGA 300


9415R-1.IR_full       301 GATGGACTACGAGATCAAGGAGCTGACAGACAGAACGGAAATACTACAGAACAAGTGCGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9415R-1.in silico     301 GATGGACTACGAGATCAAGGAGCTGACAGACAGAACGGAAATACTACAGAACAAGTGCGA 360


9415R-1.IR_full       361 TAGNCTGCAGGCCATCAACGAGTCACTGCTGGCCAAGAACCACAAGCTGGACTCGGAGCT 420
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9415R-1.in silico     361 TAGCCTGCAGGCCATCAACGAGTCACTGCTGGCCAAGAACCACAAGCTGGACTCGGAGCT 420


9415R-1.IR_full       421 GGAGCTGCTGCGCCAAGAACTCGCCGAACTGAAGCAGCAACAGCAGCACAACACCAGATG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9415R-1.in silico     421 GGAGCTGCTGCGCCAAGAACTCGCCGAACTGAAGCAGCAACAGCAGCACAACACCAGATG 480


9415R-1.IR_full       481 CATCAGCCAATCCAACGCCA 500
                          ||||||||||||||||||||
9415R-1.in silico     481 CATCAGCCAATCCAACGCCA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_079983.2	CG9415-RA, transcript variant A (Xbp1), mRNA
100	482	0	0	3	NM_166427.2	CG9415-RB, transcript variant B (Xbp1), mRNA
0.62	3	4	8	25	NM_143596.2	CG12054-RA (CG12054), mRNA
0.41	2	3	4	13	NM_131924.2	CG4857-RB (CG4857), mRNA
0.2	1	7	10	57	NM_001038742.1	CG6824-RD, transcript variant D (ovo), mRNA
0.2	1	7	10	57	NM_080338.3	CG6824-RA, transcript variant A (ovo), mRNA
0.2	1	7	10	49	NM_167026.2	CG6824-RB, transcript variant B (ovo), mRNA
0.2	1	7	10	49	NM_167027.2	CG6824-RC, transcript variant C (ovo), mRNA
0.2	1	4	6	24	NM_164960.1	CG32830-RA (CG32830), mRNA
0.2	1	3	11	58	NM_078530.2	CG11202-RA (org-1), mRNA
0.2	1	3	3	11	NM_057783.2	CG3954-RB, transcript variant B (csw), mRNA
0.2	1	3	3	8	NM_057782.3	CG3954-RA, transcript variant A (csw), mRNA
0.2	1	3	3	8	NM_166928.1	CG3954-RC, transcript variant C (csw), mRNA
0.2	1	2	5	3	NM_057716.3	CG8705-RA, transcript variant A (pnut), mRNA
0.2	1	2	5	3	NM_165597.1	CG8705-RB, transcript variant B (pnut), mRNA
0.2	1	2	3	2	NM_136089.2	CG17492-RA (mib2), mRNA
0	0	6	2	2	NM_057275.3	CG3839-RA (l(1)sc), mRNA
0	0	5	14	30	NM_132578.1	CG11146-RA (CG11146), mRNA
0	0	5	4	7	NM_139923.1	CG13675-RA (CG13675), mRNA
0	0	5	4	6	NM_057874.3	CG6202-RA, transcript variant A (Surf4), mRNA
0	0	5	4	6	NM_169647.1	CG6202-RB, transcript variant B (Surf4), mRNA
0	0	5	3	7	NM_143272.1	CG6131-RA (CG6131), mRNA
0	0	4	10	36	NM_134763.1	CG14351-RA (CG14351), mRNA
0	0	4	5	30	NM_079940.4	CG16973-RA, transcript variant A (msn), mRNA
0	0	4	5	18	NM_170258.2	CG32474-RA (dys), mRNA
0	0	4	3	7	NM_132443.1	CG16922-RA (CG16922), mRNA
0	0	3	42	362	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	3	42	362	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	3	42	362	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	3	16	57	NM_001014500.1	CG33558-RA (CG33558), mRNA

Back