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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9314R-1 |
| Symbol |
CG9314 |
Full Name |
CG9314 |
| CG No |
CG9314 |
Old CG No |
CG9314 |
| Synonyms |
CT26521, anon-WO0140519.249, CG9314 |
| Accession No (Link to NCBI) |
NM_135404.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ibaraki K, Nakatsuka M, Ohsako T, Watanabe M, Miyazaki Y, Shirakami M, Karr TL, Sanuki R, Tomaru M, Takano-Shimizu-Kouno T.
A cross-species approach for the identification of Drosophila male sterility genes.
G3 (Bethesda) (2021) 11(8)
[
PubMed ID = 34849808
]
[
RRC reference
]
Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52
[
PubMed ID = 22798488
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAGGTGCAAAGGGAGATCACG |
| Primer Seq. 3' |
aatctagaggtaccAGGGTCATGAAGTCCCAGAAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GANGTGcAAA GGGAGATCAC GACATCCAGT GGAACTCCAG TTGGCGTCAA GGATGCCATA
0061 CAGACGGTGG GACCAAGGGG TNCTGNCCTG CTGCAGGATT TCCAGTTCCT CGACGAGGTG
0121 ATGCACTTCG ACTCGGAGCG GATTNCCGAA CGGNTGGCCT ACGCCAAGGG ANGCCGGCGC
0181 CTTTGGTTAC TTCGAGTGCA CCCATGACAT TTCAAAATTC TGTGCAGCCT CCATATTCGA
0241 TAAGGTTAGA AAACGAACTG CCGTCGCGAT GAGATTCTCG GTGGCCTGCG GTGAACAGGG
0301 ATCTGCGGAT ACGGTACGTG AACAGCGCGG ATTTGCCGTC AAATTCTACA CCGACGACGG
0361 CATCTGGGAC ATTGTGGGTT GCAACATGCC CGTGCATTAT GTCCGGGATC CCATGTTGTT
0421 CCCCAGCCTG GTTCATGCCC AAAAGCGCAA TCCCCAGACC CACCTGAAGG ATCCGGACAT
0481 GTTCTGGGAC TTCATGACCC T |
| in silico PCR Fragment |
0001 GAGGTGCAAA GGGAGATCAC GACATCCAGT GGAACTCCAG TTGGCGTCAA GGATGCCATA
0061 CAGACGGTGG GACCAAGGGG TCCTGCCCTG CTGCAGGATT TCCAGTTCCT CGACGAGGTG
0121 ATGCACTTCG ACTCGGAGCG GATTCCCGAA CGGGTGGCCT ACGCCAAGGG A-GCCGGCGC
0181 CTTTGGTTAC TTCGAGTGCA CCCATGACAT TTCAAAATTC TGTGCAGCCT CCATATTCGA
0241 TAAGGTTAGA AAACGAACTG CCGTCGCGAT GAGATTCTCG GTGGCCTGCG GTGAACAGGG
0301 ATCTGCGGAT ACGGTACGTG AACAGCGCGG ATTTGCCGTC AAATTCTACA CCGACGACGG
0361 CATCTGGGAC ATTGTGGGTT GCAACATGCC CGTGCATTAT GTCCGGGATC CCATGTTGTT
0421 CCCCAGCTTG GTTCATGCCC AAAAGCGCAA TCCCCAGACC CACCTGAAGG ATCCGGACAT
0481 GTTCTGGGAC TTCATGACCC T |
| Assemble Data |
9314R-1.IR_full 1 GANGTGCAAAGGGAGATCACGACATCCAGTGGAACTCCAGTTGGCGTCAAGGATGCCATA 60
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9314R-1.in silico 1 GAGGTGCAAAGGGAGATCACGACATCCAGTGGAACTCCAGTTGGCGTCAAGGATGCCATA 60
9314R-1.IR_full 61 CAGACGGTGGGACCAAGGGGTNCTGNCCTGCTGCAGGATTTCCAGTTCCTCGACGAGGTG 120
||||||||||||||||||||| ||| ||||||||||||||||||||||||||||||||||
9314R-1.in silico 61 CAGACGGTGGGACCAAGGGGTCCTGCCCTGCTGCAGGATTTCCAGTTCCTCGACGAGGTG 120
9314R-1.IR_full 121 ATGCACTTCGACTCGGAGCGGATTNCCGAACGGNTGGCCTACGCCAAGGGANGCCGGCGC 180
|||||||||||||||||||||||| |||||||| ||||||||||||||||| ||||||||
9314R-1.in silico 121 ATGCACTTCGACTCGGAGCGGATTCCCGAACGGGTGGCCTACGCCAAGGGA-GCCGGCGC 180
9314R-1.IR_full 181 CTTTGGTTACTTCGAGTGCACCCATGACATTTCAAAATTCTGTGCAGCCTCCATATTCGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9314R-1.in silico 181 CTTTGGTTACTTCGAGTGCACCCATGACATTTCAAAATTCTGTGCAGCCTCCATATTCGA 240
9314R-1.IR_full 241 TAAGGTTAGAAAACGAACTGCCGTCGCGATGAGATTCTCGGTGGCCTGCGGTGAACAGGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9314R-1.in silico 241 TAAGGTTAGAAAACGAACTGCCGTCGCGATGAGATTCTCGGTGGCCTGCGGTGAACAGGG 300
9314R-1.IR_full 301 ATCTGCGGATACGGTACGTGAACAGCGCGGATTTGCCGTCAAATTCTACACCGACGACGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9314R-1.in silico 301 ATCTGCGGATACGGTACGTGAACAGCGCGGATTTGCCGTCAAATTCTACACCGACGACGG 360
9314R-1.IR_full 361 CATCTGGGACATTGTGGGTTGCAACATGCCCGTGCATTATGTCCGGGATCCCATGTTGTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9314R-1.in silico 361 CATCTGGGACATTGTGGGTTGCAACATGCCCGTGCATTATGTCCGGGATCCCATGTTGTT 420
9314R-1.IR_full 421 CCCCAGCCTGGTTCATGCCCAAAAGCGCAATCCCCAGACCCACCTGAAGGATCCGGACAT 480
||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
9314R-1.in silico 421 CCCCAGCTTGGTTCATGCCCAAAAGCGCAATCCCCAGACCCACCTGAAGGATCCGGACAT 480
9314R-1.IR_full 481 GTTCTGGGACTTCATGACCCT 501
|||||||||||||||||||||
9314R-1.in silico 481 GTTCTGGGACTTCATGACCCT 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_135404.2 |
CG9314-RA (CG9314), mRNA |
| 2.48 |
12 |
10 |
25 |
51 |
NM_080483.2 |
CG6871-RA (Cat), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_167203.1 |
CG1354-RB, transcript variant B (CG1354), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_132352.2 |
CG1354-RA, transcript variant A (CG1354), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_167202.2 |
CG1354-RD, transcript variant D (CG1354), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_167201.2 |
CG1354-RC, transcript variant C (CG1354), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_001031860.1 |
CG33653-RA, transcript variant A (Caps), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_001031858.1 |
CG33653-RC, transcript variant C (Caps), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_001031859.1 |
CG33653-RB, transcript variant B (Caps), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001015081.1 |
CG40477-PA.3 (CG40477), partial mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170264.1 |
CG6058-RD, transcript variant D (Ald), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079791.3 |
CG6058-RE, transcript variant E (Ald), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NR_002486.1 |
CG6058-RE, transcript variant E (Ald), mRNA, miscRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079104.2 |
CG5479-RA (mRpL43), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135852.2 |
CG4500-RA (CG4500), mRNA |
| 0 |
0 |
0 |
9 |
4 |
NM_141363.1 |
CG15589-RA (CG15589), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_136955.2 |
CG8815-RA, transcript variant A (Sin3A), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_165916.1 |
CG8815-RC, transcript variant C (Sin3A), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_165915.1 |
CG8815-RB, transcript variant B (Sin3A), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_143218.1 |
CG14239-RA (CG14239), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_078684.2 |
CG3291-RA (pcm), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_080709.3 |
CG8285-RA (boss), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079725.2 |
CG7050-RA (Nrx-1), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_057601.2 |
CG2851-RA (Gsc), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_140361.1 |
CG11263-RA (CG11263), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_139790.1 |
CG8270-RA (CG8270), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_176694.1 |
CG3126-RB, transcript variant B (C3G), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_132122.2 |
CG3126-RA, transcript variant A (C3G), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_166627.1 |
CG30176-RA (wibg), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_164773.1 |
CG7123-RB, transcript variant B (LanB1), mRNA |
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