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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9305R-1 |
| Symbol |
CG9305 |
Full Name |
CG9305 |
| CG No |
CG9305 |
Old CG No |
CG9305 |
| Synonyms |
CG9305 |
| Accession No (Link to NCBI) |
NM_135799.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATCAAGGCTCCGGCCAACCTG |
| Primer Seq. 3' |
aatctagaggtaccACGGAGGCATAGGCGAGTGCA |
| Predicted Fragment Size |
496 |
| IR fragment full Seq |
0001 NTCAAGGCTC CgGCCANCCT GTCGCTTATC AAACGCAAGC CACGCAGGGT AGGAGCTCCC
0061 AACGGCGAAA AAGGAGGATG AGGAGGAGGA GGCGGTGGTG GTGGAAGCAG CGGCTGAGTC
0121 CCCGCCCGTT GCATCGACCC CTGCTGCGGA ATTGGAACTA GAGGCTGATG CAATCGCCTT
0181 CAAGATGCCC GCAAGCTCGG CTGGGCAGCT GGAAGAGGTA TTCCACTCGG ACCTCGAGGA
0241 CAATGTCATT CAGATGGACT TGCAGAAGAC CACCAATGGT TTCCCTATGG TCGCCCAGCA
0301 AGGCTCAAGC CCGTCAGCGT GTGCGACCCA CTCCGGTATT TGGCCAGCGA CGCAACAGCT
0361 TCGTGGGCTC ACCCATGGCC AGTGATTATG AGGGCGACTA TCAGTCGCCA GCCACGCCCA
0421 CGCgGNGAGA GCGCTATCTT AGCGGTTCTT CATCGGGAAC TCCTTTGCAG CAGCAGGTGC
0481 AcNCNCNTAT GCCTCCGT |
| in silico PCR Fragment |
0001 ATCAAGGCTC CGGCCAACCT GTCGCTTATC AAACGCAAGC CACGCAGGG- AGGAGCTCCC
0061 AACGGCGAAA AAGGAGGATG AGGAGGAGGA GGCGGTGGTG GTGGAAGCAG AGGCTGAGTC
0121 CCCGCCCGTT GCATCGACCC CTGCTGCGGA ATTGGAACTA GAGGCTGATG CAATCGCCTT
0181 CAAGATGCCC GCAAGCTCGG CTGGGCAGCT GGAAGAGGTA TTCCACTCGG ACCTCGAGGA
0241 CAATGTCATT CAGATGGACT TGCAGAAGAC CACCAATGGC TTCCCTAT-G TCGCCCAGCA
0301 AGGCTCAAGC CCGTCAGCGT GTGCGACCCA CTCCGGTATT TGGCCAGCGA CGCAACAGCT
0361 TCGTGGGCTC ACCCATGGCC AGTGATTATG AGGGCGACTA TCAGTCGCCA GCCACGCCCA
0421 CGCGGCGAGA GCGCTATCTT AGCGGTTCTT CATCGGGAAC TCCTTTGCAG CAGCAGGTGC
0481 ACTCGCCTAT GCCTCCGT |
| Assemble Data |
9305R-1.IR_full 1 TCAAGGCTCCGGCCANCCTGTCGCTTATCAAACGCAAGCCACGCAGGGTAGGAGCTCCC 59
||||||||||||||| |||||||||||||||||||||||||||||||| ||||||||||
9305R-1.in silico 1 TCAAGGCTCCGGCCAACCTGTCGCTTATCAAACGCAAGCCACGCAGGG-AGGAGCTCCC 59
9305R-1.IR_full 61 AACGGCGAAAAAGGAGGATGAGGAGGAGGAGGCGGTGGTGGTGGAAGCAGCGGCTGAGTC 119
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
9305R-1.in silico 61 AACGGCGAAAAAGGAGGATGAGGAGGAGGAGGCGGTGGTGGTGGAAGCAGAGGCTGAGTC 119
9305R-1.IR_full 121 CCCGCCCGTTGCATCGACCCCTGCTGCGGAATTGGAACTAGAGGCTGATGCAATCGCCTT 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9305R-1.in silico 121 CCCGCCCGTTGCATCGACCCCTGCTGCGGAATTGGAACTAGAGGCTGATGCAATCGCCTT 179
9305R-1.IR_full 181 CAAGATGCCCGCAAGCTCGGCTGGGCAGCTGGAAGAGGTATTCCACTCGGACCTCGAGGA 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9305R-1.in silico 181 CAAGATGCCCGCAAGCTCGGCTGGGCAGCTGGAAGAGGTATTCCACTCGGACCTCGAGGA 239
9305R-1.IR_full 241 CAATGTCATTCAGATGGACTTGCAGAAGACCACCAATGGTTTCCCTATGGTCGCCCAGCA 299
||||||||||||||||||||||||||||||||||||||| |||||||| |||||||||||
9305R-1.in silico 241 CAATGTCATTCAGATGGACTTGCAGAAGACCACCAATGGCTTCCCTAT-GTCGCCCAGCA 299
9305R-1.IR_full 301 AGGCTCAAGCCCGTCAGCGTGTGCGACCCACTCCGGTATTTGGCCAGCGACGCAACAGCT 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9305R-1.in silico 301 AGGCTCAAGCCCGTCAGCGTGTGCGACCCACTCCGGTATTTGGCCAGCGACGCAACAGCT 359
9305R-1.IR_full 361 TCGTGGGCTCACCCATGGCCAGTGATTATGAGGGCGACTATCAGTCGCCAGCCACGCCCA 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9305R-1.in silico 361 TCGTGGGCTCACCCATGGCCAGTGATTATGAGGGCGACTATCAGTCGCCAGCCACGCCCA 419
9305R-1.IR_full 421 CGCGGNGAGAGCGCTATCTTAGCGGTTCTTCATCGGGAACTCCTTTGCAGCAGCAGGTGC 479
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
9305R-1.in silico 421 CGCGGCGAGAGCGCTATCTTAGCGGTTCTTCATCGGGAACTCCTTTGCAGCAGCAGGTGC 479
9305R-1.IR_full 481 ACNCNCNTATGCCTCCGT 497
|| | | |||||||||||
9305R-1.in silico 481 ACTCGCCTATGCCTCCGT 497
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
478 |
0 |
8 |
22 |
NM_135799.2 |
CG9305-RA (CG9305), mRNA |
| 0.83 |
4 |
11 |
25 |
41 |
NM_136060.1 |
CG10348-RA (CG10348), mRNA |
| 0.62 |
3 |
10 |
36 |
132 |
NM_167376.1 |
CG7107-RD, transcript variant D (up), mRNA |
| 0.62 |
3 |
10 |
36 |
132 |
NM_001014738.1 |
CG7107-RF, transcript variant F (up), mRNA |
| 0.62 |
3 |
10 |
36 |
132 |
NM_167375.1 |
CG7107-RB, transcript variant B (up), mRNA |
| 0.62 |
3 |
10 |
36 |
132 |
NM_080349.2 |
CG7107-RA, transcript variant A (up), mRNA |
| 0.62 |
3 |
10 |
36 |
132 |
NM_001014737.1 |
CG7107-RG, transcript variant G (up), mRNA |
| 0.62 |
3 |
10 |
36 |
130 |
NM_001014739.1 |
CG7107-RE, transcript variant E (up), mRNA |
| 0.62 |
3 |
7 |
10 |
38 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0.62 |
3 |
4 |
7 |
16 |
NM_079086.2 |
CG5821-RA (qkr58E-2), mRNA |
| 0.41 |
2 |
5 |
17 |
54 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0.41 |
2 |
4 |
10 |
12 |
NM_057860.3 |
CG3582-RA (U2af38), mRNA |
| 0.41 |
2 |
3 |
24 |
95 |
NM_167493.1 |
CG3606-RA, transcript variant A (caz), mRNA |
| 0.41 |
2 |
3 |
24 |
95 |
NM_078641.2 |
CG3606-RB, transcript variant B (caz), mRNA |
| 0.41 |
2 |
2 |
9 |
11 |
NM_001043084.1 |
CG34123-RD, transcript variant D (CG34123), mRNA |
| 0.41 |
2 |
2 |
9 |
11 |
NM_001043083.1 |
CG34123-RB, transcript variant B (CG34123), mRNA |
| 0.41 |
2 |
2 |
9 |
11 |
NM_001043085.1 |
CG34123-RC, transcript variant C (CG34123), mRNA |
| 0.2 |
1 |
9 |
26 |
42 |
NM_167022.1 |
CG32771-RA (CG32771), mRNA |
| 0.2 |
1 |
7 |
23 |
45 |
NM_133108.2 |
CG32542-RA (CG32542), mRNA |
| 0.2 |
1 |
7 |
15 |
33 |
NM_135967.1 |
CG13272-RA (CG13272), mRNA |
| 0.2 |
1 |
5 |
5 |
10 |
NM_164770.1 |
CG7196-RB, transcript variant B (CG7196), mRNA |
| 0.2 |
1 |
5 |
5 |
10 |
NM_135301.2 |
CG7196-RA, transcript variant A (CG7196), mRNA |
| 0.2 |
1 |
4 |
9 |
19 |
NM_166958.1 |
CG3653-RA, transcript variant A (kirre), mRNA |
| 0.2 |
1 |
4 |
9 |
19 |
NM_080320.2 |
CG3653-RB, transcript variant B (kirre), mRNA |
| 0.2 |
1 |
4 |
4 |
19 |
NM_132642.2 |
CG15744-RA (CG15744), mRNA |
| 0.2 |
1 |
4 |
4 |
14 |
NM_168293.1 |
CG32030-RA, transcript variant A (CG32030), mRNA |
| 0.2 |
1 |
4 |
4 |
14 |
NM_168294.1 |
CG32030-RB, transcript variant B (CG32030), mRNA |
| 0.2 |
1 |
3 |
18 |
23 |
NM_136895.2 |
CG13176-RA (CG13176), mRNA |
| 0.2 |
1 |
3 |
10 |
13 |
NM_132902.2 |
CG9946-RA (eIF-2alpha), mRNA |
| 0.2 |
1 |
3 |
6 |
37 |
NM_079768.2 |
CG6238-RA, transcript variant A (ssh), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| +- |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| |
|
|
|
|
|
|
