NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9223R-3

Symbol disco-r Full Name disco-related

CG No CG32577 Old CG No CG9223

Synonyms CG9223, CG9219, CG32577, disco-r

Accession No (Link to NCBI) NM_167487.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGGCTCGATGCCCGCCAATTT

Primer Seq. 3'

aatctagaggtaccTCTTCGTCCTCATCGCCGTCA

Predicted Fragment Size

IR fragment full Seq

0001 GGGCTCGATG CCCGCCAATT TGGGCACCCA GTTCATCAAT CCGGTGACgG GCAAAAAGCG 
0061 AGTGCAGTGC AATGTCTGTC TGAAGACCTT TTGCGACAAG GGTGCTCTAA AGATTCACTT 
0121 TTCGGCGGTG CATCTCAGGG AGATGcACAA GTGCACGGTG GATGGATGCA GCATGATGTT 
0181 TAGCTCGAGG CGATCGAGGA ATCGCCATAG CGCCAATCCG AATCCAAAGC TCCATTCGCC 
0241 GCATCTGCGA CGCAAGATCT CGCCGCACGA TGGACGCAGT GCGCAACCGC ATCCGCTTTT 
0301 GCTTCAGGCG CCCAATGGTC TCATGGCGGG ACTGGCACCA TTCGGCAGCT TTCCGCTGCT 
0361 GACGCCGCCA CCGGATTTGC GGCATCATGC GATGGGTGGT TCGGGCGCGG GATCAGGAGC 
0421 GGGAGTCGGT GCTCTGGAGT TGAAACACGG ACAGGATTAT CTGCAGAGAT CGTATTTGGA 
0481 TGCGGGACGA TTCGAGGCGA CAGCGACGTA AACTGGCGAT GGAGAACGAG CACACTGAAG 
0541 ATGAGGACGA CGACGAGATA CTGGAGGTTG GCATCCACAT GGCCGGCGAC GATGATGACG 
0601 ACGAGGCTGA CGGCGATGAG GACGAAGA

in silico PCR Fragment

0001 GGGCTCGATG CCCGCCAATT TGGGCACCCA GTTCATCAAT CCGGTGACGG GCAAAAAGCG 
0061 AGTGCAGTGC AATGTCTGTC TGAAGACCTT TTGCGACAAG GGTGCTCTAA AGATTCACTT 
0121 TTCGGCGGTG CATCTCAGGG AGATGCACAA GTGCACGGTG GATGGATGCA GCATGATGTT 
0181 TAGCTCGAGG CGATCGAGGA ATCGCCATAG CGCCAATCCG AATCCAAAGC TCCATTCGCC 
0241 GCATCTGCGA CGCAAGATCT CGCCGCACGA TGGACGCAGT GCGCAACCGC ATCCGCTTTT 
0301 GCTTCAGGCG CCCAATGGTC TCATGGCGGG ACTGGCACCA TTCGGCAGCT TTCCGCTGCT 
0361 GACGCCGCCA CCGGATTTGC GGCATCATGC GATGGGTGGT TCGGGCGCGG GATCAGGAGC 
0421 GGGAGTCGGT GCTCTGGAGT TGAAACACGG ACAGGATTAT CTGCAGAGAT CGTATTTGGA 
0481 TGCGGGACGA TTCGAGG-GA CAGCGACGTA AACTGGCGAT GGAGAACGAG CACACTGAAG 
0541 ATGAGGACGA CGACGAGATA CTGGAGGTTG GCATCCACAT GGCCGGCGAC GATGATGACG 
0601 ACGAGGCTGA CGGCGATGAG GACGAAGA

Assemble Data


9223R-3.IR_full       1   GGGCTCGATGCCCGCCAATTTGGGCACCCAGTTCATCAATCCGGTGACGGGCAAAAAGCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     1   GGGCTCGATGCCCGCCAATTTGGGCACCCAGTTCATCAATCCGGTGACGGGCAAAAAGCG 60


9223R-3.IR_full       61  AGTGCAGTGCAATGTCTGTCTGAAGACCTTTTGCGACAAGGGTGCTCTAAAGATTCACTT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     61  AGTGCAGTGCAATGTCTGTCTGAAGACCTTTTGCGACAAGGGTGCTCTAAAGATTCACTT 120


9223R-3.IR_full       121 TTCGGCGGTGCATCTCAGGGAGATGCACAAGTGCACGGTGGATGGATGCAGCATGATGTT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     121 TTCGGCGGTGCATCTCAGGGAGATGCACAAGTGCACGGTGGATGGATGCAGCATGATGTT 180


9223R-3.IR_full       181 TAGCTCGAGGCGATCGAGGAATCGCCATAGCGCCAATCCGAATCCAAAGCTCCATTCGCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     181 TAGCTCGAGGCGATCGAGGAATCGCCATAGCGCCAATCCGAATCCAAAGCTCCATTCGCC 240


9223R-3.IR_full       241 GCATCTGCGACGCAAGATCTCGCCGCACGATGGACGCAGTGCGCAACCGCATCCGCTTTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     241 GCATCTGCGACGCAAGATCTCGCCGCACGATGGACGCAGTGCGCAACCGCATCCGCTTTT 300


9223R-3.IR_full       301 GCTTCAGGCGCCCAATGGTCTCATGGCGGGACTGGCACCATTCGGCAGCTTTCCGCTGCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     301 GCTTCAGGCGCCCAATGGTCTCATGGCGGGACTGGCACCATTCGGCAGCTTTCCGCTGCT 360


9223R-3.IR_full       361 GACGCCGCCACCGGATTTGCGGCATCATGCGATGGGTGGTTCGGGCGCGGGATCAGGAGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     361 GACGCCGCCACCGGATTTGCGGCATCATGCGATGGGTGGTTCGGGCGCGGGATCAGGAGC 420


9223R-3.IR_full       421 GGGAGTCGGTGCTCTGGAGTTGAAACACGGACAGGATTATCTGCAGAGATCGTATTTGGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     421 GGGAGTCGGTGCTCTGGAGTTGAAACACGGACAGGATTATCTGCAGAGATCGTATTTGGA 480


9223R-3.IR_full       481 TGCGGGACGATTCGAGGCGACAGCGACGTAAACTGGCGATGGAGAACGAGCACACTGAAG 540
                          ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     481 TGCGGGACGATTCGAGG-GACAGCGACGTAAACTGGCGATGGAGAACGAGCACACTGAAG 540


9223R-3.IR_full       541 ATGAGGACGACGACGAGATACTGGAGGTTGGCATCCACATGGCCGGCGACGATGATGACG 600
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9223R-3.in silico     541 ATGAGGACGACGACGAGATACTGGAGGTTGGCATCCACATGGCCGGCGACGATGATGACG 600


9223R-3.IR_full       601 ACGAGGCTGACGGCGATGAGGACGAAGA 628
                          ||||||||||||||||||||||||||||
9223R-3.in silico     601 ACGAGGCTGACGGCGATGAGGACGAAGA 628

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	609	0	2	25	NM_167487.1	CG32577-RA (disco-r), mRNA
3.94	24	37	37	57	NM_078638.2	CG9908-RA, transcript variant A (disco), mRNA
3.94	24	37	37	57	NM_001038758.1	CG9908-RB, transcript variant B (disco), mRNA
0.49	3	2	3	13	NM_137848.2	CG3732-RA (CG3732), mRNA
0	0	8	24	96	NM_132831.1	CG8184-RB (CG8184), mRNA
0	0	5	5	6	NM_140802.2	CG6877-RA (Aut1), mRNA
0	0	4	18	48	NM_001032052.1	CG33715-RE, transcript variant E (Msp-300), mRNA
0	0	3	12	10	NM_170161.1	CG31125-RA (CG31125), mRNA
0	0	3	7	55	NM_132413.1	CG15295-RA (CG15295), mRNA
0	0	3	4	6	NM_138150.2	CG16778-RA, transcript variant A (Tkr), mRNA
0	0	3	4	6	NM_001014554.1	CG16778-RD, transcript variant D (Tkr), mRNA
0	0	3	4	6	NM_079141.2	CG16778-RB, transcript variant B (Tkr), mRNA
0	0	3	4	6	NM_145761.1	CG16778-RC, transcript variant C (Tkr), mRNA
0	0	3	3	13	NM_134895.2	CG3605-RA (CG3605), mRNA
0	0	3	2	16	NM_167215.1	CG15312-RB, transcript variant B (CG15312), mRNA
0	0	3	2	16	NM_132373.2	CG15312-RA, transcript variant A (CG15312), mRNA
0	0	3	2	16	NM_167216.1	CG15312-RC, transcript variant C (CG15312), mRNA
0	0	3	2	7	NM_141274.1	CG2669-RA (CG2669), mRNA
0	0	2	12	21	NM_079635.2	CG6384-RA, transcript variant A (Cp190), mRNA
0	0	2	12	21	NM_169632.1	CG6384-RB, transcript variant B (Cp190), mRNA
0	0	2	11	52	NM_143226.1	CG5467-RA (CG5467), mRNA
0	0	2	11	11	NM_143769.3	CG9432-RA, transcript variant A (l(2)01289), mRNA
0	0	2	10	21	NM_168628.1	CG7656-RA, transcript variant A (CG7656), mRNA
0	0	2	10	21	NM_168627.1	CG7656-RC, transcript variant C (CG7656), mRNA
0	0	2	10	21	NM_140526.3	CG7656-RB, transcript variant B (CG7656), mRNA
0	0	2	10	17	NM_078530.2	CG11202-RA (org-1), mRNA
0	0	2	8	45	NM_131924.2	CG4857-RB (CG4857), mRNA
0	0	2	8	17	NM_136251.2	CG9246-RA (CG9246), mRNA
0	0	2	7	18	NM_167022.1	CG32771-RA (CG32771), mRNA
0	0	2	7	8	NM_136710.2	CG1381-RA (CG1381), mRNA

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