National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9196R-2 
 Symbol CG9196  Full Name CG9196 
 CG No CG9196  Old CG No CG9196 
 Synonyms BcDNA:LP10725, CG9196 
 Accession No (Link to NCBI) NM_138117.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGCTTTCTGCTGCTGGCCCAA 
 Primer Seq. 3' 
aatctagaggtaccCTGTACTCGTTGGACTCCACG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 aGCTTTCTGC TGCTGGCCCA ATTGGGTTTC TCCCAGCCGC AGCAGCAGNN TCGCCATCTG 
0061 ACTACGGTGA GTGAGCAGCC TCCGGAGGGT TACTACGCCT TCGTCGAGTC CCCGAATGCG 
0121 GTGCCTCCCA AGGTCAGGCC GCCACCTTAC ACCTTTGTAA ATGCGGAGTG CAAGGACGTG 
0181 GNCGCCGGCA AGAAGTCTGC CGTGTCCGTT CACAACATTT GCGGAGATCT AAACAAGGGT 
0241 CAGATACCCA AGAATCCGCT GGGACAAAAT GTACTCGGGG AGCCCTATCC CTTCGAACTT 
0301 ATTCGCAACC AAACGCTGAA GTTCCTTTCG AAAACTTTGC CCGTTCTAAA GGCCGACGAT 
0361 ACCCTACCCA AGGTCACCCA GATCATTCGA GATGAACCCG TGGAACAGCT GGACAGCAAC 
0421 AACATCGACA GGCCCTATCC CGCAGGAGGT TCCACTCgCG TCCGCCGCAG NCtgCCGGAG 
0481 GGCGTGGAGT CCAACGAGTA CAg 
 in silico PCR Fragment 
0001 AGCTTTCTGC TGCTGGCCCA ATTGGGTTTC TCCCAGCCGC AGCAGCAG-- TCGCCATCTG 
0061 ACTACGGTGA G-GAGCAGCC TCCGGAGGGT TATTACGCCT TCGTCGAGTC CCCGAATGCG 
0121 GTGCCTCCCA AGGTCAGGCC GCCACCTTAC ACCTTTGTAA ATGCGGAGTG CAAGGACGTG 
0181 GCCGCCGGCA AGAAGTCTGC CGTGTCCGTT CACAACATTT GCGGAGATCT AAACAAGGGT 
0241 CAGATACCCA AGAATCCGCT GGGACAAAAT GTACTCGGGG AGCCCTATCC CTTCGAACTT 
0301 ATTCGCAACC AAACGCTGAA GTTCCTTTCG AAAACTTTGC CCGTTCTAAA GGCCGACGAT 
0361 ACCCTACCCA AGGTCACCCA GATCATTCGA GATGAACCCG TGGAACAGCT GGACAGCAAC 
0421 AACATCGACA GGCCCTATCC CGCAGGAGGT TCCACTCGCG TCCGCCGCAG CCTGCCGGAG 
0481 GGCGTGGAGT CCAACGAGTA CAG 
 Assemble Data 

9196R-2.IR_full       1   AGCTTTCTGCTGCTGGCCCAATTGGGTTTCTCCCAGCCGCAGCAGCAGNNTCGCCATCTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||  ||||||||||
9196R-2.in silico     1   AGCTTTCTGCTGCTGGCCCAATTGGGTTTCTCCCAGCCGCAGCAGCAG--TCGCCATCTG 60


9196R-2.IR_full       61  ACTACGGTGAGTGAGCAGCCTCCGGAGGGTTACTACGCCTTCGTCGAGTCCCCGAATGCG 120
                          ||||||||||| |||||||||||||||||||| |||||||||||||||||||||||||||
9196R-2.in silico     61  ACTACGGTGAG-GAGCAGCCTCCGGAGGGTTATTACGCCTTCGTCGAGTCCCCGAATGCG 120


9196R-2.IR_full       121 GTGCCTCCCAAGGTCAGGCCGCCACCTTACACCTTTGTAAATGCGGAGTGCAAGGACGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9196R-2.in silico     121 GTGCCTCCCAAGGTCAGGCCGCCACCTTACACCTTTGTAAATGCGGAGTGCAAGGACGTG 180


9196R-2.IR_full       181 GNCGCCGGCAAGAAGTCTGCCGTGTCCGTTCACAACATTTGCGGAGATCTAAACAAGGGT 240
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9196R-2.in silico     181 GCCGCCGGCAAGAAGTCTGCCGTGTCCGTTCACAACATTTGCGGAGATCTAAACAAGGGT 240


9196R-2.IR_full       241 CAGATACCCAAGAATCCGCTGGGACAAAATGTACTCGGGGAGCCCTATCCCTTCGAACTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9196R-2.in silico     241 CAGATACCCAAGAATCCGCTGGGACAAAATGTACTCGGGGAGCCCTATCCCTTCGAACTT 300


9196R-2.IR_full       301 ATTCGCAACCAAACGCTGAAGTTCCTTTCGAAAACTTTGCCCGTTCTAAAGGCCGACGAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9196R-2.in silico     301 ATTCGCAACCAAACGCTGAAGTTCCTTTCGAAAACTTTGCCCGTTCTAAAGGCCGACGAT 360


9196R-2.IR_full       361 ACCCTACCCAAGGTCACCCAGATCATTCGAGATGAACCCGTGGAACAGCTGGACAGCAAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9196R-2.in silico     361 ACCCTACCCAAGGTCACCCAGATCATTCGAGATGAACCCGTGGAACAGCTGGACAGCAAC 420


9196R-2.IR_full       421 AACATCGACAGGCCCTATCCCGCAGGAGGTTCCACTCGCGTCCGCCGCAGNCTGCCGGAG 480
                          |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
9196R-2.in silico     421 AACATCGACAGGCCCTATCCCGCAGGAGGTTCCACTCGCGTCCGCCGCAGCCTGCCGGAG 480


9196R-2.IR_full       481 GGCGTGGAGTCCAACGAGTACAG 503
                          |||||||||||||||||||||||
9196R-2.in silico     481 GGCGTGGAGTCCAACGAGTACAG 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_138117.2  CG9196-RA (CG9196), mRNA 
0.2   1  3  2  5  NM_167080.1  CG32742-RA (l(1)G0148), mRNA 
0.2   1  2  2  4  NM_164696.1  CG31635-RA (CG31635), mRNA 
0   0  3  6  3  NM_143304.1  CG13980-RA (CG13980), mRNA 
0   0  3  2  3  NM_139541.1  CG12017-RA (CG12017), mRNA 
0   0  2  7  24  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
0   0  2  7  24  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
0   0  2  6  6  NM_206509.1  CG8053-RB, transcript variant B (eIF-1A), mRNA 
0   0  2  6  6  NM_079989.2  CG8053-RA, transcript variant A (eIF-1A), mRNA 
0   0  2  3  5  NM_143353.1  CG12425-RA (CG12425), mRNA 
0   0  2  2  24  NM_001038742.1  CG6824-RD, transcript variant D (ovo), mRNA 
0   0  2  2  24  NM_080338.3  CG6824-RA, transcript variant A (ovo), mRNA 
0   0  2  2  16  NM_167027.2  CG6824-RC, transcript variant C (ovo), mRNA 
0   0  2  2  16  NM_167026.2  CG6824-RB, transcript variant B (ovo), mRNA 
0   0  2  2  3  NM_165262.2  CG31753-RA (ham), mRNA 
0   0  2  2  2  NM_133024.2  CG6769-RA (CG6769), mRNA 
0   0  2  2  2  NM_001042847.1  CG40486-RB, transcript variant B (CG40486), mRNA 
0   0  2  2  2  NM_001042846.1  CG40486-RA, transcript variant A (CG40486), mRNA 
0   0  1  12  10  NM_001043265.1  CG34139-RA (CG34139), mRNA 
0   0  1  7  17  NM_001043134.1  CG7368-RB (CG7368), mRNA 
0   0  1  4  20  NM_079939.2  CG5580-RA, transcript variant A (sbb), mRNA 
0   0  1  4  9  NM_134552.3  CG1412-RA (RhoGAP19D), mRNA 
0   0  1  4  6  NM_142140.1  CG17304-RA (CG17304), mRNA 
0   0  1  4  4  NM_140316.2  CG32104-RB (CG32104), mRNA 
0   0  1  3  11  NM_176254.1  CG5403-RB, transcript variant B (retn), mRNA 
0   0  1  3  11  NM_057516.3  CG5403-RA, transcript variant A (retn), mRNA 
0   0  1  3  11  NM_079909.2  CG3327-RA, transcript variant A (E23), mRNA 
0   0  1  3  11  NM_205900.1  CG3327-RC, transcript variant C (E23), mRNA 
0   0  1  3  8  NM_164830.3  CG18405-RA, transcript variant A (Sema-1a), mRNA 
0   0  1  3  7  NM_169947.1  CG17299-RF, transcript variant F (SNF4Agamma), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+- 
DC
+ 
DM
 
VM
 
VB
+ 
Caspase-3 signal
N
+ 
DB
+ 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.