NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9084R-2

Symbol CG9084 Full Name CG9084

CG No CG9084 Old CG No CG9084

Synonyms CG9084

Accession No (Link to NCBI) NM_136796.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGTTGCAAAACAACGCGGTG

Primer Seq. 3'

aatctagaggtaccCTAGACTTTTTGCCTGGCAGC

Predicted Fragment Size

IR fragment full Seq

0001 ATGTTGCAAA ACAACGCGGT GCGCACTTTG CAATACGACA GTGAGATCCG TGTGGCCCAG 
0061 CAGCCAAGTC ACTCAGAACC GAGAGTTTAT GACTCCCATA TCAGCATTAC CTCACAACCC 
0121 AGGCCGGAGN CTCCACGCAT CCCGCTGCCA GTGCCAATTG CCACGGTTAC GGGNAAGCCG 
0181 GACGCTTATC CCACTTTCCG GATACGATTG CCTAGCAGAT CTGCCGTCGG TACACATTGA 
0241 ACAGACCTTT GAGCTTAACG ATGCCCTAAC AGGCGTCTCC TCGGAGAATC GATATGTGGT 
0301 GCGATCTCCA CTGGGTGATG CCATTTTCGC GGCCAACGAG AGTTCCACAG AAAAAAATCG 
0361 ACTACTTTGG GGAGCTGGTC GACCCTTTCA GATGCACCTG CTGGATAAAA CGCACCAGGA 
0421 AGCTCTGGTT TTTCGCAAAA AACTAGCGAT GGGATCCATG TGCTGCCAGG CAAAAAGTCT 
0481 AG

in silico PCR Fragment

0001 ATGTTGCAAA ACAACGCGGT GCGCACTTTG CAATACGACA GTGAGATCCG TGTGGCCCAG 
0061 CAGCCAAGTC TCTCAGAACC GAGAGTTTAT GACTCCCATA TCAGCATTAC CTCACAACCC 
0121 AGGCCGGAGG CTCCACGCAT CCCGCTGCCA GTGCCAATTG CCACGGTTAC GGG-AAGCCG 
0181 GACGCTTATC CCACTTTCCG GATACGATTG CCTAGCAGAT CTGCCGTCCG TACATATTGA 
0241 ACAGACCTTT GAGCTTAACG ATGCCCTAAC AGGCGTCTCC TCGGAGAATC GATATGTGGT 
0301 GCGATCTCCA CTGGGTGATG CCATTTTCGC GGCCAACGAG AGTTCCACAG AAAAAAATCG 
0361 ACTACTTTGG GGAGCTGGTC GACCCTTTCA GATGCACCTG CTGGATAAAA CGCACCAGGA 
0421 AGCTCTGGTT TTTCGCAAAA AACTAGCGAT GGGATCCATG TGCTGCCAGG CAAAAAGTCT 
0481 AG

Assemble Data


9084R-2.IR_full       1   ATGTTGCAAAACAACGCGGTGCGCACTTTGCAATACGACAGTGAGATCCGTGTGGCCCAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9084R-2.in silico     1   ATGTTGCAAAACAACGCGGTGCGCACTTTGCAATACGACAGTGAGATCCGTGTGGCCCAG 60


9084R-2.IR_full       61  CAGCCAAGTCACTCAGAACCGAGAGTTTATGACTCCCATATCAGCATTACCTCACAACCC 120
                          |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
9084R-2.in silico     61  CAGCCAAGTCTCTCAGAACCGAGAGTTTATGACTCCCATATCAGCATTACCTCACAACCC 120


9084R-2.IR_full       121 AGGCCGGAGNCTCCACGCATCCCGCTGCCAGTGCCAATTGCCACGGTTACGGGNAAGCCG 180
                          ||||||||| ||||||||||||||||||||||||||||||||||||||||||| ||||||
9084R-2.in silico     121 AGGCCGGAGGCTCCACGCATCCCGCTGCCAGTGCCAATTGCCACGGTTACGGG-AAGCCG 180


9084R-2.IR_full       181 GACGCTTATCCCACTTTCCGGATACGATTGCCTAGCAGATCTGCCGTCGGTACACATTGA 240
                          |||||||||||||||||||||||||||||||||||||||||||||||| ||||| |||||
9084R-2.in silico     181 GACGCTTATCCCACTTTCCGGATACGATTGCCTAGCAGATCTGCCGTCCGTACATATTGA 240


9084R-2.IR_full       241 ACAGACCTTTGAGCTTAACGATGCCCTAACAGGCGTCTCCTCGGAGAATCGATATGTGGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9084R-2.in silico     241 ACAGACCTTTGAGCTTAACGATGCCCTAACAGGCGTCTCCTCGGAGAATCGATATGTGGT 300


9084R-2.IR_full       301 GCGATCTCCACTGGGTGATGCCATTTTCGCGGCCAACGAGAGTTCCACAGAAAAAAATCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9084R-2.in silico     301 GCGATCTCCACTGGGTGATGCCATTTTCGCGGCCAACGAGAGTTCCACAGAAAAAAATCG 360


9084R-2.IR_full       361 ACTACTTTGGGGAGCTGGTCGACCCTTTCAGATGCACCTGCTGGATAAAACGCACCAGGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9084R-2.in silico     361 ACTACTTTGGGGAGCTGGTCGACCCTTTCAGATGCACCTGCTGGATAAAACGCACCAGGA 420


9084R-2.IR_full       421 AGCTCTGGTTTTTCGCAAAAAACTAGCGATGGGATCCATGTGCTGCCAGGCAAAAAGTCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9084R-2.in silico     421 AGCTCTGGTTTTTCGCAAAAAACTAGCGATGGGATCCATGTGCTGCCAGGCAAAAAGTCT 480


9084R-2.IR_full       481 AG 482
                          ||
9084R-2.in silico     481 AG 482

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	463	0	0	2	NM_136796.2	CG9084-RB (CG9084), mRNA
0	0	2	2	4	NM_001014755.1	CG33525-RD, transcript variant D (CG33525), mRNA
0	0	1	6	5	NM_079817.2	CG1842-RA (Dhc98D), mRNA
0	0	1	4	3	NM_141213.2	CG9805-RA, transcript variant A (eIF3-S10), mRNA
0	0	1	4	3	NM_168999.2	CG9805-RB, transcript variant B (eIF3-S10), mRNA
0	0	1	3	5	NM_143104.1	CG11856-RA (Nup358), mRNA
0	0	1	2	6	NM_170281.1	CG5476-RA (CG5476), mRNA
0	0	1	2	4	NM_080337.2	CG6899-RA, transcript variant A (Ptp4E), mRNA
0	0	1	2	4	NM_167024.1	CG6899-RB, transcript variant B (Ptp4E), mRNA
0	0	1	2	3	NM_001015187.1	CG40178-PA.3 (CG40178), mRNA
0	0	1	2	2	NM_135106.1	CG7236-RA (CG7236), mRNA
0	0	1	2	2	NM_165890.2	CG13160-RA (CG13160), mRNA
0	0	0	6	2	NM_138134.1	CG18506-RA (CG18506), mRNA
0	0	0	5	5	NM_080142.2	CG1925-RA (mus205), mRNA
0	0	0	5	4	NM_164790.1	CG31605-RG, transcript variant G (Bsg), mRNA
0	0	0	4	2	NM_168029.1	CG12076-RB, transcript variant B (YT521-B), mRNA
0	0	0	4	2	NM_139554.3	CG12076-RA, transcript variant A (YT521-B), mRNA
0	0	0	3	6	NM_175960.3	CG33196-RB (dp), mRNA
0	0	0	3	5	NM_176718.1	CG9113-RD, transcript variant D (AP-1gamma), mRNA
0	0	0	3	5	NM_167178.2	CG9113-RB, transcript variant B (AP-1gamma), mRNA
0	0	0	3	5	NM_176717.1	CG9113-RC, transcript variant C (AP-1gamma), mRNA
0	0	0	3	5	NM_132299.3	CG9113-RA, transcript variant A (AP-1gamma), mRNA
0	0	0	3	5	NM_206671.1	CG9113-RE, transcript variant E (AP-1gamma), mRNA
0	0	0	3	4	NM_165146.1	CG4894-RC, transcript variant C (Ca-alpha1D), mRNA
0	0	0	3	4	NM_080365.2	CG4894-RB, transcript variant B (Ca-alpha1D), mRNA
0	0	0	3	4	NM_134429.2	CG4894-RA, transcript variant A (Ca-alpha1D), mRNA
0	0	0	3	4	NM_165147.1	CG4894-RD, transcript variant D (Ca-alpha1D), mRNA
0	0	0	3	4	NM_137812.2	CG11291-RA (CG11291), mRNA
0	0	0	3	3	NM_135563.1	CG5322-RA (CG5322), mRNA
0	0	0	3	2	NM_164771.2	CG7179-RA (CG7179), mRNA

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