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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8967R-4 |
| Symbol |
otk |
Full Name |
off-track |
| CG No |
CG8967 |
Old CG No |
CG8967 |
| Synonyms |
OTK, Otk, Dtrk, dTrk, CT25769, OFT/TRK, CG8967, DTrk, Trk48D, Tk48D, IGGtyk, Dtkr, anon-WO2004063362.81, otk |
| Accession No (Link to NCBI) |
NM_078981.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAATCCGTGAAATTCGAGTGC |
| Primer Seq. 3' |
aatctagaggtaccTGGTAGACATAGCCCTGCTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NAATCCGTGA AATTCGAGTG CGAATCGACC GACTCCTACA GTGAACTGCA CTACGACTGG
0061 TTGCATAATG GTCATCGGAT TGCGTATGAC AAGCGTGTCC ATCAAATCGG TTCCAATTTG
0121 CATATCGAGG CGGTGCGGCG CACGGAGGAT GTTGGCAACT ATGTTTGCAT CGCGACAAAT
0181 TTGGCCAGCG GCGCCAGGGA GGCGTCTCCG CCCGCCAAAT TGAGCGTGAT ATATCTTGAA
0241 TCGGCCAGTG TNCaGCTGCT GGGCAGCAAT CGCAACGAGC TGCTGCTCAA GTGCCACGTG
0301 GAGGGCGCCT CAGGAGATTT GGAACCGCTA GAGATCGAAT GGTANCGCAA CAGCGAGAAA
0361 CTTAGCACCT GGAAGAACGT CCAGCTGGAT CAGCATCGCC TGATAATCCG ACAGCCGGGA
0421 AGCGAGGACG ATGGCCTGTA CCGCTGCACc gCCTCCAATG CGGCGGGTCG AGTGATGAGC
0481 AAGCAGGGCT ATGTCTACCA |
| in silico PCR Fragment |
0001 GAATCCGTGA AATTCGAGTG CGAATCGACC GACTCCTACA GTGAACTGCA CTACGACTGG
0061 TTGCATAATG GTCATCGGAT TGCGTATGAC AAGCGTGTCC ATCAAATCGG TTCCAATTTG
0121 CATATCGAGG CGGTGCGGCG CACGGAGGAT GTTGGCAACT ATGTTTGCAT CGCGACAAAT
0181 TTGGCCAGCG GCGCCAGGGA GGCGTCTCCG CCCGCCAAAC TGAGCGTGAT ATATCTTGAA
0241 TCGGCCAGTG TTCAGCTGCT GGGCAGCAAT CGCAACGAGC TGCTGCTCAA GTGCCACGTG
0301 GAGGGCGCCT CAGGAGATTT GGAACCGCTA GAGATCGAAT GGTATCGCAA CAGCGAGAAA
0361 CTTAGCACCT GGAAGAACGT CCAGCTGGAT CAGCATCGCC TGATAATCCG ACAGCCGGGA
0421 AGCGAGGACG ATGGCCTGTA CCGCTGCACC GCCTCCAATG CGGCGGGTCG AGTGATGAGC
0481 AAGCAGGGCT ATGTCTACCA |
| Assemble Data |
8967R-4.IR_full 1 AATCCGTGAAATTCGAGTGCGAATCGACCGACTCCTACAGTGAACTGCACTACGACTGG 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8967R-4.in silico 1 AATCCGTGAAATTCGAGTGCGAATCGACCGACTCCTACAGTGAACTGCACTACGACTGG 59
8967R-4.IR_full 61 TTGCATAATGGTCATCGGATTGCGTATGACAAGCGTGTCCATCAAATCGGTTCCAATTTG 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8967R-4.in silico 61 TTGCATAATGGTCATCGGATTGCGTATGACAAGCGTGTCCATCAAATCGGTTCCAATTTG 119
8967R-4.IR_full 121 CATATCGAGGCGGTGCGGCGCACGGAGGATGTTGGCAACTATGTTTGCATCGCGACAAAT 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8967R-4.in silico 121 CATATCGAGGCGGTGCGGCGCACGGAGGATGTTGGCAACTATGTTTGCATCGCGACAAAT 179
8967R-4.IR_full 181 TTGGCCAGCGGCGCCAGGGAGGCGTCTCCGCCCGCCAAATTGAGCGTGATATATCTTGAA 239
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
8967R-4.in silico 181 TTGGCCAGCGGCGCCAGGGAGGCGTCTCCGCCCGCCAAACTGAGCGTGATATATCTTGAA 239
8967R-4.IR_full 241 TCGGCCAGTGTNCAGCTGCTGGGCAGCAATCGCAACGAGCTGCTGCTCAAGTGCCACGTG 299
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
8967R-4.in silico 241 TCGGCCAGTGTTCAGCTGCTGGGCAGCAATCGCAACGAGCTGCTGCTCAAGTGCCACGTG 299
8967R-4.IR_full 301 GAGGGCGCCTCAGGAGATTTGGAACCGCTAGAGATCGAATGGTANCGCAACAGCGAGAAA 359
|||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
8967R-4.in silico 301 GAGGGCGCCTCAGGAGATTTGGAACCGCTAGAGATCGAATGGTATCGCAACAGCGAGAAA 359
8967R-4.IR_full 361 CTTAGCACCTGGAAGAACGTCCAGCTGGATCAGCATCGCCTGATAATCCGACAGCCGGGA 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8967R-4.in silico 361 CTTAGCACCTGGAAGAACGTCCAGCTGGATCAGCATCGCCTGATAATCCGACAGCCGGGA 419
8967R-4.IR_full 421 AGCGAGGACGATGGCCTGTACCGCTGCACCGCCTCCAATGCGGCGGGTCGAGTGATGAGC 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8967R-4.in silico 421 AGCGAGGACGATGGCCTGTACCGCTGCACCGCCTCCAATGCGGCGGGTCGAGTGATGAGC 479
8967R-4.IR_full 481 AAGCAGGGCTATGTCTACCA 499
||||||||||||||||||||
8967R-4.in silico 481 AAGCAGGGCTATGTCTACCA 499
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_078981.2 |
CG8967-RA (otk), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_139907.2 |
CG8254-RA (exex), mRNA |
| 0 |
0 |
4 |
3 |
6 |
NM_132642.2 |
CG15744-RA (CG15744), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_165298.1 |
CG10237-RC, transcript variant C (CG10237), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_136123.2 |
CG10237-RB, transcript variant B (CG10237), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_165299.1 |
CG10237-RA, transcript variant A (CG10237), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_137328.2 |
CG9010-RA (CG9010), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_170244.1 |
CG17370-RB, transcript variant B (CG17370), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_143180.1 |
CG17370-RA, transcript variant A (CG17370), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_170245.1 |
CG17370-RC, transcript variant C (CG17370), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_167038.1 |
CG3239-RB, transcript variant B (CG3239), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_131998.2 |
CG3239-RA, transcript variant A (CG3239), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_142403.1 |
CG17801-RA (CG17801), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169674.1 |
CG31293-RA (rec), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_078765.2 |
CG9115-RA (mtm), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_080535.2 |
CG9774-RA (rok), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_169386.1 |
CG17228-RA, transcript variant A (pros), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_079593.3 |
CG17228-RC, transcript variant C (pros), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_176459.1 |
CG17228-RD, transcript variant D (pros), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043023.1 |
CG17800-PAQ (Dscam), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043061.1 |
CG17800-PAD (Dscam), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043053.1 |
CG17800-PAG (Dscam), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043025.1 |
CG17800-PAZ (Dscam), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_166064.1 |
CG30480-RA (CG30480), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043034.1 |
CG17800-PBA (Dscam), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167199.1 |
CG32697-RD, transcript variant D (l(1)G0232), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014728.1 |
CG32697-RF, transcript variant F (l(1)G0232), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167198.1 |
CG32697-RB, transcript variant B (l(1)G0232), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132348.2 |
CG32697-RA, transcript variant A (l(1)G0232), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167197.1 |
CG32697-RC, transcript variant C (l(1)G0232), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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