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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8954R-4 |
| Symbol |
Smg5 |
Full Name |
Smg5 |
| CG No |
CG8954 |
Old CG No |
CG8954 |
| Synonyms |
CG8954, SMG5, anon-34Ea, unnamed, BG:DS08220.2, Smg5 |
| Accession No (Link to NCBI) |
NM_135841.4 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Park J, Lee J, Kim JH, Lee J, Park H, Lim C.
ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.
Nucleic Acids Res (2021) 49(19) 11294-11311
[
PubMed ID = 34551427
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGAGGTGACATTCAGCTCG |
| Primer Seq. 3' |
aatctagaggtaccATCTCTTCCATGCGTGCCGAC |
| Predicted Fragment Size |
473 |
| IR fragment full Seq |
0001 ATGGAGGTGA CATTCAGCTC GGGCGGATCA TCGAACGCCT CCAGCGAGTG CGCCATAGAC
0061 GGCGGCACGA ATCGCTGTCG CGGCTTGGAG CCCAACAATG GAACCTGCAT CCTCAGCCAG
0121 GAGGTAAAGG ATCTTTACAG ATCCCTCTAC ACCGCCTCCA AGCAACTGGA CGATGCCAAG
0181 AGGAATGTGC AGTCGGTGGG ACAGCTGTTC CAGCACGAAA TCGAAGAGAA ACGCTCGCTC
0241 CTAGTGCAGC TGTGCAAGCA GATCATCTTC AAGGACTACC AGTCGGTGGG CAAGAAGGTG
0301 CGCGAGGTGA TGTGGCGACG TGGATACTAC GAGTTCATTG CCTTCGTGAA GAAGAACTGG
0361 CACAAGCAGT GCCAGGATGC GGCCACGGCC CAAGAGAACA TGCAAAAGCT GGAGCGATTT
0421 CCTCTGCGCT GGCATCTTCA ACTACAAACG ATTGTCGGCA CGCATGGAAG AGAT |
| in silico PCR Fragment |
0001 ATGGAGGTGA CATTCAGCTC GGGCGGATCA TCGAACGCCT CCAGCGAGTG CGCCATAGAC
0061 GGCGGCACGA ATCGCTGTCG CGGCTTGGAG CCCAACAATG GAACCTGCAT CCTCAGCCAG
0121 GAGGTAAAGG ATCTTTACAG ATCCCTCTAC ACCGCCTCCA AGCAACTGGA CGATGCCAAG
0181 AGGAATGTGC AGTCGGTGGG ACAGCTGTTC CAGCACGAAA TCGAAGAGAA ACGCTCGCTC
0241 CTAGTGCAGC TGTGCAAGCA GATCATCTTC AAGGACTACC AGTCGGTGGG CAAGAAGGTG
0301 CGCGAGGTGA TGTGGCGACG TGGATACTAC GAGTTCATTG CCTTCGTGAA GAAGAACTGG
0361 CACAAGCAGT GCCAGGATGC GGCCACGGCC CAAGAGAACA TGCAAAAGCT GGAGCGA-TT
0421 CCTCTGCGCT GGCATCTTCA ACTACAAACG ATTGTCGGCA CGCATGGAAG AGAT |
| Assemble Data |
8954R-4.IR_full 1 ATGGAGGTGACATTCAGCTCGGGCGGATCATCGAACGCCTCCAGCGAGTGCGCCATAGAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8954R-4.in silico 1 ATGGAGGTGACATTCAGCTCGGGCGGATCATCGAACGCCTCCAGCGAGTGCGCCATAGAC 60
8954R-4.IR_full 61 GGCGGCACGAATCGCTGTCGCGGCTTGGAGCCCAACAATGGAACCTGCATCCTCAGCCAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8954R-4.in silico 61 GGCGGCACGAATCGCTGTCGCGGCTTGGAGCCCAACAATGGAACCTGCATCCTCAGCCAG 120
8954R-4.IR_full 121 GAGGTAAAGGATCTTTACAGATCCCTCTACACCGCCTCCAAGCAACTGGACGATGCCAAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8954R-4.in silico 121 GAGGTAAAGGATCTTTACAGATCCCTCTACACCGCCTCCAAGCAACTGGACGATGCCAAG 180
8954R-4.IR_full 181 AGGAATGTGCAGTCGGTGGGACAGCTGTTCCAGCACGAAATCGAAGAGAAACGCTCGCTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8954R-4.in silico 181 AGGAATGTGCAGTCGGTGGGACAGCTGTTCCAGCACGAAATCGAAGAGAAACGCTCGCTC 240
8954R-4.IR_full 241 CTAGTGCAGCTGTGCAAGCAGATCATCTTCAAGGACTACCAGTCGGTGGGCAAGAAGGTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8954R-4.in silico 241 CTAGTGCAGCTGTGCAAGCAGATCATCTTCAAGGACTACCAGTCGGTGGGCAAGAAGGTG 300
8954R-4.IR_full 301 CGCGAGGTGATGTGGCGACGTGGATACTACGAGTTCATTGCCTTCGTGAAGAAGAACTGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8954R-4.in silico 301 CGCGAGGTGATGTGGCGACGTGGATACTACGAGTTCATTGCCTTCGTGAAGAAGAACTGG 360
8954R-4.IR_full 361 CACAAGCAGTGCCAGGATGCGGCCACGGCCCAAGAGAACATGCAAAAGCTGGAGCGATTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
8954R-4.in silico 361 CACAAGCAGTGCCAGGATGCGGCCACGGCCCAAGAGAACATGCAAAAGCTGGAGCGA-TT 420
8954R-4.IR_full 421 CCTCTGCGCTGGCATCTTCAACTACAAACGATTGTCGGCACGCATGGAAGAGAT 474
||||||||||||||||||||||||||||||||||||||||||||||||||||||
8954R-4.in silico 421 CCTCTGCGCTGGCATCTTCAACTACAAACGATTGTCGGCACGCATGGAAGAGAT 474
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
455 |
0 |
0 |
0 |
NM_135841.4 |
CG8954-RA, transcript variant A (Smg5), mRNA |
| 100 |
455 |
0 |
0 |
0 |
NM_165069.1 |
CG8954-RB, transcript variant B (Smg5), mRNA |
| 0 |
0 |
4 |
3 |
5 |
NM_135224.1 |
CG11201-RA (CG11201), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_139379.2 |
CG13929-RB, transcript variant B (metl), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_167907.1 |
CG13929-RA, transcript variant A (metl), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_001043270.1 |
CG5621-RB, transcript variant B (CG5621), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_142670.2 |
CG5621-RA, transcript variant A (CG5621), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_142954.3 |
CG6057-RA (SMC1), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_167965.1 |
CG32296-RA (Mrtf), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_176527.1 |
CG32491-RU, transcript variant U (mod(mdg4)), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_080013.2 |
CG6050-RA (EfTuM), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_080337.2 |
CG6899-RA, transcript variant A (Ptp4E), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_167024.1 |
CG6899-RB, transcript variant B (Ptp4E), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_168497.1 |
CG32105-RB (CG32105), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079134.5 |
CG9071-RA, transcript variant A (NaCP60E), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_166696.1 |
CG9071-RB, transcript variant B (NaCP60E), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140257.1 |
CG7283-RA, transcript variant A (RpL10Ab), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_168481.1 |
CG7283-RC, transcript variant C (RpL10Ab), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_168480.1 |
CG7283-RB, transcript variant B (RpL10Ab), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_164778.1 |
CG7356-RB, transcript variant B (CG7356), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_135330.1 |
CG7356-RA, transcript variant A (CG7356), mRNA |
| 0 |
0 |
1 |
2 |
12 |
NM_138049.2 |
CG3328-RA (CG3328), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_137583.3 |
CG11228-RA (hpo), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_170329.1 |
CG5930-RC, transcript variant C (TfIIA-L), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_057647.3 |
CG5930-RA, transcript variant A (TfIIA-L), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_057648.3 |
CG5930-RB, transcript variant B (TfIIA-L), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_057534.3 |
CG3242-RA (sob), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_001042800.1 |
CG32699-RB, transcript variant B (CG32699), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_137296.2 |
CG8306-RA (CG8306), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_132342.2 |
CG32699-RA, transcript variant A (CG32699), mRNA |
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