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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8934R-1 |
| Symbol |
CG8934 |
Full Name |
CG8934 |
| CG No |
CG8934 |
Old CG No |
CG8934 |
| Synonyms |
CG8934 |
| Accession No (Link to NCBI) |
NM_170251.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Hosono C, Matsuda R, Adryan B, Samakovlis C.
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.
Nat Cell Biol (2015) 17(12) 1569-76
[
PubMed ID = 26551273
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGTGGGAGCCATTTTCTTCGT |
| Primer Seq. 3' |
aatctagaggtaccCAGCGGCTAGGGAATTCATGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GNTGGGAGCC ATTTTCTTCG TCATCATCAA GGGCACCGTC GATTTGGGCG GATTGGGCGT
0061 GGTTATTCAG CGGAATTTCG ACACCGGACG CATGGAGTGG CCTGAATTGA CGCTGGATCC
0121 CAAGGTGCGA ATGTCCATGT TGGCAATGAT GGTGGGCAAC GTGGCACACA ACTCGTATAA
0181 CCTCGGATGC AATCAGATCA TCACCCAGCG ATACCTGTCA CTGCCCGGAT TAAAGCAGAT
0241 GGCCCAGTGC TCGTTCCTCT TCATTTCCGG ATTGGTTCTT CTGATGGCCA TCTGCATGTA
0301 CAACGGCCTC CTGCTATCCG CAACCTACTA CGACTGTGAT CCATTGACCA CGAAGTTGGC
0361 GGTGGCCAAG GATCAATTGG TTCCCCTGCT CGTGGTGCAA TCCATGAGCG CATTTCCCGG
0421 AGTTCCCGGC ATGTTTGTGG CCGGAGTGTT TAGTGCCGCC CTCAGTTCTC TGTCCACGGG
0481 CATGAATTCC CTAGCCGCTG |
| in silico PCR Fragment |
0001 GGTGGGAGCC ATTTTCTTCG TCATCATCAA GGGCACCGTC GATTTGGGCG GATTGGGCGT
0061 GGTTATTCAG CGGAATTTCG ACACCGGACG CATGGAGTGG CCTGAATTGA CGCTGGATCC
0121 CAAGGTGCGA ATGTCCATGT TGGCAATGAT GGTGGGCAAC GTGGCACACA ACTCGTATAA
0181 CCTCGGATGC AATCAGATCA TCACCCAGCG ATACCTGTCA CTGCCCGGAT TAAAGCAGAT
0241 GGCCCAGTGC TCGTTCCTCT TCATTTCCGG ATTGGTTCTT CTGATGGCCA TCTGCATGTA
0301 CAACGGCCTC CTGCTATCCG CAACCTACTA CGACTGTGAT CCATTGACCA CGAAGTTGGC
0361 GGTGGCCAAG GATCAATTGG TTCCCCTGCT CGTGGTGCAA TCCATGAGCG CATTTCCCGG
0421 AGTTCCCGGC ATGTTTGTGG CCGGAGTGTT TAGTGCCGCC CTCAGTTCTC TGTCCACGGG
0481 CATGAATTCC CTAGCCGCTG |
| Assemble Data |
8934R-1.IR_full 1 GNTGGGAGCCATTTTCTTCGTCATCATCAAGGGCACCGTCGATTTGGGCGGATTGGGCGT 60
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8934R-1.in silico 1 GGTGGGAGCCATTTTCTTCGTCATCATCAAGGGCACCGTCGATTTGGGCGGATTGGGCGT 60
8934R-1.IR_full 61 GGTTATTCAGCGGAATTTCGACACCGGACGCATGGAGTGGCCTGAATTGACGCTGGATCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8934R-1.in silico 61 GGTTATTCAGCGGAATTTCGACACCGGACGCATGGAGTGGCCTGAATTGACGCTGGATCC 120
8934R-1.IR_full 121 CAAGGTGCGAATGTCCATGTTGGCAATGATGGTGGGCAACGTGGCACACAACTCGTATAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8934R-1.in silico 121 CAAGGTGCGAATGTCCATGTTGGCAATGATGGTGGGCAACGTGGCACACAACTCGTATAA 180
8934R-1.IR_full 181 CCTCGGATGCAATCAGATCATCACCCAGCGATACCTGTCACTGCCCGGATTAAAGCAGAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8934R-1.in silico 181 CCTCGGATGCAATCAGATCATCACCCAGCGATACCTGTCACTGCCCGGATTAAAGCAGAT 240
8934R-1.IR_full 241 GGCCCAGTGCTCGTTCCTCTTCATTTCCGGATTGGTTCTTCTGATGGCCATCTGCATGTA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8934R-1.in silico 241 GGCCCAGTGCTCGTTCCTCTTCATTTCCGGATTGGTTCTTCTGATGGCCATCTGCATGTA 300
8934R-1.IR_full 301 CAACGGCCTCCTGCTATCCGCAACCTACTACGACTGTGATCCATTGACCACGAAGTTGGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8934R-1.in silico 301 CAACGGCCTCCTGCTATCCGCAACCTACTACGACTGTGATCCATTGACCACGAAGTTGGC 360
8934R-1.IR_full 361 GGTGGCCAAGGATCAATTGGTTCCCCTGCTCGTGGTGCAATCCATGAGCGCATTTCCCGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8934R-1.in silico 361 GGTGGCCAAGGATCAATTGGTTCCCCTGCTCGTGGTGCAATCCATGAGCGCATTTCCCGG 420
8934R-1.IR_full 421 AGTTCCCGGCATGTTTGTGGCCGGAGTGTTTAGTGCCGCCCTCAGTTCTCTGTCCACGGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8934R-1.in silico 421 AGTTCCCGGCATGTTTGTGGCCGGAGTGTTTAGTGCCGCCCTCAGTTCTCTGTCCACGGG 480
8934R-1.IR_full 481 CATGAATTCCCTAGCCGCTG 500
||||||||||||||||||||
8934R-1.in silico 481 CATGAATTCCCTAGCCGCTG 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_170251.1 |
CG8934-RA (CG8934), mRNA |
| 10.16 |
49 |
66 |
34 |
39 |
NM_143187.1 |
CG8932-RA (CG8932), mRNA |
| 0 |
0 |
2 |
20 |
19 |
NM_170250.1 |
CG31090-RA (CG31090), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_165115.1 |
CG31733-RA, transcript variant A (ms(2)35Ci), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_165114.1 |
CG31733-RB, transcript variant B (ms(2)35Ci), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_057261.2 |
CG3821-RA (Aats-asp), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_078983.2 |
CG8439-RA, transcript variant A (Cct5), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_135445.1 |
CG3769-RA (CG3769), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168090.1 |
CG32242-RA (CG32242), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_078761.3 |
CG7234-RI (GluRIIB), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_169654.2 |
CG31302-RC, transcript variant C (CG31302), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_169652.2 |
CG31302-RA, transcript variant A (CG31302), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_169653.2 |
CG31302-RB, transcript variant B (CG31302), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_135615.2 |
CG6700-RA (CG6700), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_078722.2 |
CG17941-RA (ds), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137655.2 |
CG30149-RB (rig), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_144142.2 |
CG12158-RA (CG12158), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043263.1 |
CG34157-RF, transcript variant F (Dys), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043257.1 |
CG34157-RH, transcript variant H (Dys), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043259.1 |
CG34157-RC, transcript variant C (Dys), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043258.1 |
CG34157-RA, transcript variant A (Dys), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001043261.1 |
CG34157-RG, transcript variant G (Dys), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_057977.3 |
CG9239-RA, transcript variant A (B4), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165046.1 |
CG9239-RB, transcript variant B (B4), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143125.1 |
CG10559-RA (CG10559), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169378.1 |
CG4863-RB, transcript variant B (RpL3), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140457.1 |
CG13474-RA (CG13474), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164933.1 |
CG7438-RB, transcript variant B (Myo31DF), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_078814.2 |
CG7438-RA, transcript variant A (Myo31DF), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079592.2 |
CG4863-RA, transcript variant A (RpL3), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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