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NIG-RNAi Strains Detail |
Request : Click the "Order" button to request for this stock |
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
Stock ID |
8864R-4 |
Symbol |
Cyp28a5 |
Full Name |
Cyp28a5 |
CG No |
CG8864 |
Old CG No |
CG8864 |
Synonyms |
28a5, cyp28a5, gh10483, BG:DS00180.11, DS00180.11, BEST:GH10483, CG8864, Cyp28a5 |
Accession No (Link to NCBI) |
NM_135850.2 |
Inserted Chr. |
lll |
Insertional Mutation |
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Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
Map Viewer |
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Reference
[Please submit your publication]
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Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T. RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc. Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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Comment |
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Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
Primer Seq. 5' |
aaggcctacatggccggaccgCGATGCTGTGGGCATTTTTGG |
Primer Seq. 3' |
aatctagaggtaccGCGATGGGAAGGAACTAGTCA |
Predicted Fragment Size |
500 |
IR fragment full Seq |
0001 CGATGCTGTG GGCATTTTTG GCAGCCgNTC TCCACAGCTG CTGGTGATCA ATCCCGCGTT
0061 GGCGcNTCGA GTCTTTGTGT CCAACTTTAA GAACTTCCAC GACAATGAGA TCGCCAAGAA
0121 TATTGACGAG AAAACCGATT TCATTTTCGC CAACAATCCG TTCTCGTTAA CTGGCGAAAA
0181 GTGGAAAACT CGAAGGGCGG ACGTAACGCC TGGTCTTACG ATGGGACGGA TAAAGACAGT
0241 CTATCCGGTG ACAAACAAGG TGTGCCAAAA GTTGACCGAG TGGGTGGAGA AGCAATTGCG
0301 TCTGGGTTCT AAAGACGGCA TCGATGCCAA GCACATGAGT CTCTGCTTCA CAACAGAGAT
0361 GGTCACCGAT TGTGTTTTGG GTCTGGGCGC TGAAAGCTTT TCGGATAAGC CCACACCCAT
0421 AATGTCGAAA ATCAATGATC TCTTCAACCA GCCCTGGACC TTTGTGCTGT TCTTTATACT
0481 GACTAGTTCC TTCCCAtCGN |
in silico PCR Fragment |
0001 CGATGCTGTG GGCATTTTTG GCAGCCGCTC TCCACAGCTG CTGGTGATCA ATCCCGCGTT
0061 GGCGCGTCGA GTCTTTGTGT CCAACTTTAA GAACTTCCAC GACAATGAGA TCGCCAAGAA
0121 TATTGACGAG AAAACCGATT TCATTTTCGC CAACAATCCG TTCTCGTTAA CTGGCGAAAA
0181 GTGGAAAACT CGAAGGGCGG ACGTAACGCC TGGTCTTACG ATGGGACGGA TAAAGACAGT
0241 CTATCCGGTG ACAAACAAGG TGTGCCAAAA GTTGACCGAG TGGGTGGAGA AGCAATTGCG
0301 TCTGGGTTCT AAAGACGGCA TCGATGCCAA GCACATGAGT CTCTGCTTCA CAACAGAGAT
0361 GGTCACCGAT TGTGTTTTGG GTCTGGGCGC TGAAAGCTTT TCGGATAAGC CCACACCCAT
0421 AATGTCGAAA ATCAATGATC TCTTCAACCA GCCCTGGACC TTTGTGCTGT TCTTTATACT
0481 GACTAGTTCC TTCCCATCGC |
Assemble Data |
8864R-4.IR_full 1 CGATGCTGTGGGCATTTTTGGCAGCCGNTCTCCACAGCTGCTGGTGATCAATCCCGCGTT 60
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8864R-4.in silico 1 CGATGCTGTGGGCATTTTTGGCAGCCGCTCTCCACAGCTGCTGGTGATCAATCCCGCGTT 60
8864R-4.IR_full 61 GGCGCNTCGAGTCTTTGTGTCCAACTTTAAGAACTTCCACGACAATGAGATCGCCAAGAA 120
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8864R-4.in silico 61 GGCGCGTCGAGTCTTTGTGTCCAACTTTAAGAACTTCCACGACAATGAGATCGCCAAGAA 120
8864R-4.IR_full 121 TATTGACGAGAAAACCGATTTCATTTTCGCCAACAATCCGTTCTCGTTAACTGGCGAAAA 180
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8864R-4.in silico 121 TATTGACGAGAAAACCGATTTCATTTTCGCCAACAATCCGTTCTCGTTAACTGGCGAAAA 180
8864R-4.IR_full 181 GTGGAAAACTCGAAGGGCGGACGTAACGCCTGGTCTTACGATGGGACGGATAAAGACAGT 240
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8864R-4.in silico 181 GTGGAAAACTCGAAGGGCGGACGTAACGCCTGGTCTTACGATGGGACGGATAAAGACAGT 240
8864R-4.IR_full 241 CTATCCGGTGACAAACAAGGTGTGCCAAAAGTTGACCGAGTGGGTGGAGAAGCAATTGCG 300
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8864R-4.in silico 241 CTATCCGGTGACAAACAAGGTGTGCCAAAAGTTGACCGAGTGGGTGGAGAAGCAATTGCG 300
8864R-4.IR_full 301 TCTGGGTTCTAAAGACGGCATCGATGCCAAGCACATGAGTCTCTGCTTCACAACAGAGAT 360
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8864R-4.in silico 301 TCTGGGTTCTAAAGACGGCATCGATGCCAAGCACATGAGTCTCTGCTTCACAACAGAGAT 360
8864R-4.IR_full 361 GGTCACCGATTGTGTTTTGGGTCTGGGCGCTGAAAGCTTTTCGGATAAGCCCACACCCAT 420
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8864R-4.in silico 361 GGTCACCGATTGTGTTTTGGGTCTGGGCGCTGAAAGCTTTTCGGATAAGCCCACACCCAT 420
8864R-4.IR_full 421 AATGTCGAAAATCAATGATCTCTTCAACCAGCCCTGGACCTTTGTGCTGTTCTTTATACT 480
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8864R-4.in silico 421 AATGTCGAAAATCAATGATCTCTTCAACCAGCCCTGGACCTTTGTGCTGTTCTTTATACT 480
8864R-4.IR_full 481 GACTAGTTCCTTCCCATCGN 500
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8864R-4.in silico 481 GACTAGTTCCTTCCCATCGC 500
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Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
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cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
100 |
482 |
0 |
0 |
0 |
NM_135850.2 |
CG8864-RA (Cyp28a5), mRNA |
0 |
0 |
6 |
7 |
13 |
NM_135068.2 |
CG10833-RA (Cyp28d1), mRNA |
0 |
0 |
2 |
5 |
4 |
NM_140176.1 |
CG6279-RA (CG6279), mRNA |
0 |
0 |
1 |
7 |
2 |
NM_079615.2 |
CG7756-RA (Hsc70-2), mRNA |
0 |
0 |
1 |
3 |
2 |
NM_057560.3 |
CG17369-RB, transcript variant B (Vha55), mRNA |
0 |
0 |
1 |
3 |
2 |
NM_169475.1 |
CG17369-RA, transcript variant A (Vha55), mRNA |
0 |
0 |
1 |
2 |
5 |
NM_136335.2 |
CG1298-RA (CG1298), mRNA |
0 |
0 |
1 |
2 |
3 |
NM_141573.2 |
CG8236-RA (CG8236), mRNA |
0 |
0 |
0 |
12 |
9 |
NM_135067.1 |
CG6081-RA (Cyp28d2), mRNA |
0 |
0 |
0 |
6 |
5 |
NM_137162.1 |
CG10253-RA (CG10253), mRNA |
0 |
0 |
0 |
6 |
4 |
NM_135036.2 |
CG8873-RA, transcript variant A (CG8873), mRNA |
0 |
0 |
0 |
6 |
4 |
NM_001042867.1 |
CG8873-RB, transcript variant B (CG8873), mRNA |
0 |
0 |
0 |
6 |
1 |
NM_141756.3 |
CG31272-RA (CG31272), mRNA |
0 |
0 |
0 |
5 |
7 |
NM_140491.2 |
CG13458-RA (CG13458), mRNA |
0 |
0 |
0 |
5 |
5 |
NM_137595.1 |
CG11025-RA, transcript variant A (isopeptidase-T-3), mRNA |
0 |
0 |
0 |
5 |
5 |
NM_166373.2 |
CG11025-RB, transcript variant B (isopeptidase-T-3), mRNA |
0 |
0 |
0 |
5 |
4 |
NM_143136.2 |
CG11910-RA (CG11910), mRNA |
0 |
0 |
0 |
4 |
7 |
NM_169222.2 |
CG31462-RA (CG31462), mRNA |
0 |
0 |
0 |
4 |
5 |
NM_079071.2 |
CG9277-RB, transcript variant B (betaTub56D), mRNA |
0 |
0 |
0 |
4 |
5 |
NM_166356.1 |
CG9277-RA, transcript variant A (betaTub56D), mRNA |
0 |
0 |
0 |
4 |
3 |
NM_206266.1 |
CG12008-RB, transcript variant B (kst), mRNA |
0 |
0 |
0 |
4 |
3 |
NM_206265.1 |
CG12008-RC, transcript variant C (kst), mRNA |
0 |
0 |
0 |
4 |
3 |
NM_079176.1 |
CG12008-RA, transcript variant A (kst), mRNA |
0 |
0 |
0 |
3 |
6 |
NM_138254.2 |
CG9153-RB, transcript variant B (CG9153), mRNA |
0 |
0 |
0 |
3 |
6 |
NM_167868.1 |
CG9153-RA, transcript variant A (CG9153), mRNA |
0 |
0 |
0 |
3 |
5 |
NM_142218.1 |
CG18522-RA (CG18522), mRNA |
0 |
0 |
0 |
3 |
4 |
NM_166019.1 |
CG18076-RH, transcript variant H (shot), mRNA |
0 |
0 |
0 |
3 |
4 |
NM_139981.2 |
CG6831-RA (rhea), mRNA |
0 |
0 |
0 |
3 |
4 |
NM_137569.3 |
CG11961-RB, transcript variant B (CG11961), mRNA |
0 |
0 |
0 |
3 |
4 |
NM_166342.1 |
CG11961-RA, transcript variant A (CG11961), mRNA |
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Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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