NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8858R-4

Symbol CG8858 Full Name CG8858

CG No CG8858 Old CG No CG8858

Synonyms CG8858

Accession No (Link to NCBI)

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGAAATTGAGCTTCTGGAGCG

Primer Seq. 3'

aatctagaggtaccCCTGGAGCAGTGCCAAAAAGT

Predicted Fragment Size

IR fragment full Seq

0001 AGAAATTGAG CTTCTGGAGC GCGTCCTGTT GCGTCTGGGA AATGCGGACT CGGATGAGAA 
0061 GCTGGAANCG CTGTGGGCAA GTTCCTGACC CCGGTGATCC TCAAAATGAA CTCGCCCCAC 
0121 AATGTGGTGC GCACAAAGGT CGTAGAAGTG CTCACCCATA TCAAGAGGCG CCTGTCGTCG 
0181 CGCGGACATG TGCAAATACC CGTAGAGGCA CTGCTGGATC AGTATGCCGC CCAGGATAGC 
0241 AGCACCTTCC TCAAGAACTT CGCCATCATA TTCATTACCA TGGGCTTTCC GCGCCTGGNG 
0301 TTGGAACAAC AGACTGCGCT GGCCAGCAAA GTGGTGGGCT GCGAGGACAA GCTGGAGAGC 
0361 TACCAGGACA AGCTGTTCGC CCTGCTGCTG CCCGTACTGT CGGACATGAA GATTCCCGAC 
0421 GACCCGACGC AGCGCTCAAA GCTACTTGAT CTGCAGNACA AGCCAGCCAT CTGTGCAAAC 
0481 TTTTTGGNAC TGCTCCANG

in silico PCR Fragment

0001 AGAAATTGAG CTTCTGGAGC GCGTCCTGTT GCGTCTGGGA AATGCGGACT CGGATGAGAA 
0061 GCTGGAAGCC GCTGTGGGCA AGTTCCTGAC CCCGGTGATC CTCAAAATGA ACTCGCCCCA 
0121 CAATGTGGTG CGCACAAAGG TCGTAGAAGT GCTCACCCAT ATCAAGAGGC GCCTGTCGTC 
0181 GCGCGGACAT GTGCAAATAC CCGTAGAGGC ACTGCTGGAT CAGTATGCCG CCCAGGATAG 
0241 CAGCACCTTC CTCAAGAACT TCGCCATCAT ATTCATTACC ATGGGCTTTC CGCGCCTGGC 
0301 GTTGGAACAA CAGACTGCGC TGGCCAGCAA AGTGGTGGGC TGCGAGGACA AGCTGGAGAG 
0361 CTACCAGGAC AAGCTGTTCG CCCTGCTGCT GCCCGTACTG TCGGACATGA AGATTCCCGA 
0421 CGACCCGACG CAGCGCTCAA AGCTACTTGA TCTGCAGGAC AAGCCAGCCA TCTGTGCAAA 
0481 CTTTTTGGCA CTGCTCCAGG

Assemble Data


8858R-4.IR full       1   AGAAATTGAGCTTCTGGAGCGCGTCCTGTTGCGTCTGGGAAATGCGGACTCGGATGAGAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8858R-4.in silico     1   AGAAATTGAGCTTCTGGAGCGCGTCCTGTTGCGTCTGGGAAATGCGGACTCGGATGAGAA 60


8858R-4.IR full       61  GCTGGAANC-GCTGTGGGCAAGTTCCTGACCCCGGTGATCCTCAAAATGAACTCGCCCCA 120
                          ||||||| | ||||||||||||||||||||||||||||||||||||||||||||||||||
8858R-4.in silico     61  GCTGGAAGCCGCTGTGGGCAAGTTCCTGACCCCGGTGATCCTCAAAATGAACTCGCCCCA 120


8858R-4.IR full       121 CAATGTGGTGCGCACAAAGGTCGTAGAAGTGCTCACCCATATCAAGAGGCGCCTGTCGTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8858R-4.in silico     121 CAATGTGGTGCGCACAAAGGTCGTAGAAGTGCTCACCCATATCAAGAGGCGCCTGTCGTC 180


8858R-4.IR full       181 GCGCGGACATGTGCAAATACCCGTAGAGGCACTGCTGGATCAGTATGCCGCCCAGGATAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8858R-4.in silico     181 GCGCGGACATGTGCAAATACCCGTAGAGGCACTGCTGGATCAGTATGCCGCCCAGGATAG 240


8858R-4.IR full       241 CAGCACCTTCCTCAAGAACTTCGCCATCATATTCATTACCATGGGCTTTCCGCGCCTGGN 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
8858R-4.in silico     241 CAGCACCTTCCTCAAGAACTTCGCCATCATATTCATTACCATGGGCTTTCCGCGCCTGGC 300


8858R-4.IR full       301 GTTGGAACAACAGACTGCGCTGGCCAGCAAAGTGGTGGGCTGCGAGGACAAGCTGGAGAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8858R-4.in silico     301 GTTGGAACAACAGACTGCGCTGGCCAGCAAAGTGGTGGGCTGCGAGGACAAGCTGGAGAG 360


8858R-4.IR full       361 CTACCAGGACAAGCTGTTCGCCCTGCTGCTGCCCGTACTGTCGGACATGAAGATTCCCGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8858R-4.in silico     361 CTACCAGGACAAGCTGTTCGCCCTGCTGCTGCCCGTACTGTCGGACATGAAGATTCCCGA 420


8858R-4.IR full       421 CGACCCGACGCAGCGCTCAAAGCTACTTGATCTGCAGNACAAGCCAGCCATCTGTGCAAA 480
                          ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
8858R-4.in silico     421 CGACCCGACGCAGCGCTCAAAGCTACTTGATCTGCAGGACAAGCCAGCCATCTGTGCAAA 480


8858R-4.IR full       481 CTTTTTGGNACTGCTCCANG 500
                          |||||||| ||||||||| |
8858R-4.in silico     481 CTTTTTGGCACTGCTCCAGG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

Wing Disc