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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8852R-2 
 Symbol CG8852  Full Name CG8852 
 CG No CG8852  Old CG No CG8852 
 Synonyms CT9284, CG8852 
 Accession No (Link to NCBI) NM_134946.3 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTTTTCGGTGGCGATGACGCA 
 Primer Seq. 3' 
aatctagaggtaccGTGGCAAGTCCTGGATAGTTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TTTTTCGGTG GCGATGACgN AGTGCgNCTT CCTCGTTGTC GTATCGCAGA TTTTGTCACA 
0061 CGTGCGGGCA GcTGGCGATG CGATGTCTTC CGCCAACGAA TCAATATCGT TGCCACCGGC 
0121 TTGCGTCATT CGGGCAACGC TCTATAAGAC ATCTTCGTCG GAAAGGAGTC AAAACGATTT 
0181 TGGGCTGCCT CTGGCAATAG ATTGCAGTGA GAACAGTAGC ATGGGGATGA ATTACTTCGA 
0241 TTTGGGCGCC CAAAGGAGTG GCCGGAAAGA GGCACGTTTC GCTGGATGGC TTTCAGACAC 
0301 CACCCTTGGG AATCTCGAGC TATGGCTTGG AGTACCTGGA CAATTGTGTG GATCTGGAAA 
0361 GTGTCGAGAT CCAACGTTTT GTGGNCGATG CCACCCTGAA ACTGAGCTGT GGAGGAGCTC 
0421 TGATCCCCAA CCTGACCGCA GTATCTTTCA NATACAACGA GCTGGGTACA TTGAGTGGAG 
0481 AAACTATCCA GGACTTGCCA C 
 in silico PCR Fragment 
0001 TTTTTCGGTG GCGATGACGC AGTGCGTCTT CCTCGTTGTC GTATCGCAGA TTTTGTCACA 
0061 CGTGCGGGCA GCTGGCGATG CGATGTCTTC CGCCAACGAA TCAATATCGT TGCCACCGGC 
0121 TTGCGTCATT CGGGCAACGC TCTATAAGAC ATCTTCGTCG GAAAGGAGTC AAAACGATTT 
0181 TGGGCTGCCT CTGGCAATAG ATTGCAGTGA GAACAGTAGC ATGGGGATGA ATTACTTCGA 
0241 TTTGGGCGCC CAAA-GAGTG GCCGGAAAGA GGCACGTTTC GCTGGATGGC TTTCAGACAC 
0301 CACCCTTGGG AATCTCGAGC TATGGCTTGG AGTACCTGGA CAATTGTGTG GATCTGGAAA 
0361 GTGTCGAGAT CCAACGTTTT GTGGGCGATG CCACCCTGAA ACTGAGCTGT GGAGGAGCTC 
0421 TGATCCCCAA CCTGACCGCA GTATCTTTCA GATACAACGA GCTGGGTACA TTGAGTGGAG 
0481 AAACTATCCA GGACTTGCCA C 
 Assemble Data 

8852R-2.IR_full       1   TTTTTCGGTGGCGATGACGNAGTGCGNCTTCCTCGTTGTCGTATCGCAGATTTTGTCACA 60
                          ||||||||||||||||||| |||||| |||||||||||||||||||||||||||||||||
8852R-2.in silico     1   TTTTTCGGTGGCGATGACGCAGTGCGTCTTCCTCGTTGTCGTATCGCAGATTTTGTCACA 60


8852R-2.IR_full       61  CGTGCGGGCAGCTGGCGATGCGATGTCTTCCGCCAACGAATCAATATCGTTGCCACCGGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8852R-2.in silico     61  CGTGCGGGCAGCTGGCGATGCGATGTCTTCCGCCAACGAATCAATATCGTTGCCACCGGC 120


8852R-2.IR_full       121 TTGCGTCATTCGGGCAACGCTCTATAAGACATCTTCGTCGGAAAGGAGTCAAAACGATTT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8852R-2.in silico     121 TTGCGTCATTCGGGCAACGCTCTATAAGACATCTTCGTCGGAAAGGAGTCAAAACGATTT 180


8852R-2.IR_full       181 TGGGCTGCCTCTGGCAATAGATTGCAGTGAGAACAGTAGCATGGGGATGAATTACTTCGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8852R-2.in silico     181 TGGGCTGCCTCTGGCAATAGATTGCAGTGAGAACAGTAGCATGGGGATGAATTACTTCGA 240


8852R-2.IR_full       241 TTTGGGCGCCCAAAGGAGTGGCCGGAAAGAGGCACGTTTCGCTGGATGGCTTTCAGACAC 300
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
8852R-2.in silico     241 TTTGGGCGCCCAAA-GAGTGGCCGGAAAGAGGCACGTTTCGCTGGATGGCTTTCAGACAC 300


8852R-2.IR_full       301 CACCCTTGGGAATCTCGAGCTATGGCTTGGAGTACCTGGACAATTGTGTGGATCTGGAAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8852R-2.in silico     301 CACCCTTGGGAATCTCGAGCTATGGCTTGGAGTACCTGGACAATTGTGTGGATCTGGAAA 360


8852R-2.IR_full       361 GTGTCGAGATCCAACGTTTTGTGGNCGATGCCACCCTGAAACTGAGCTGTGGAGGAGCTC 420
                          |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
8852R-2.in silico     361 GTGTCGAGATCCAACGTTTTGTGGGCGATGCCACCCTGAAACTGAGCTGTGGAGGAGCTC 420


8852R-2.IR_full       421 TGATCCCCAACCTGACCGCAGTATCTTTCANATACAACGAGCTGGGTACATTGAGTGGAG 480
                          |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
8852R-2.in silico     421 TGATCCCCAACCTGACCGCAGTATCTTTCAGATACAACGAGCTGGGTACATTGAGTGGAG 480


8852R-2.IR_full       481 AAACTATCCAGGACTTGCCAC 501
                          |||||||||||||||||||||
8852R-2.in silico     481 AAACTATCCAGGACTTGCCAC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_134946.3  CG8852-RA (CG8852), mRNA 
0   0  3  3  4  NM_167282.2  CG1725-RA, transcript variant A (dlg1), mRNA 
0   0  3  3  3  NM_078565.3  CG1725-RD, transcript variant D (dlg1), mRNA 
0   0  3  3  3  NM_206682.2  CG1725-RG, transcript variant G (dlg1), mRNA 
0   0  3  3  3  NM_206684.2  CG1725-RE, transcript variant E (dlg1), mRNA 
0   0  3  2  3  NM_134706.1  CG3862-RA (CG3862), mRNA 
0   0  2  2  4  NM_142577.1  CG4583-RA (ire-1), mRNA 
0   0  2  2  2  NM_001038795.1  CG8683-RA (CG8683), mRNA 
0   0  1  4  3  NM_133154.2  CG12199-RA, transcript variant A (kek5), mRNA 
0   0  1  4  3  NM_167647.2  CG12199-RB, transcript variant B (kek5), mRNA 
0   0  1  4  3  NM_078967.2  CG12387-RA (zetaTry), mRNA 
0   0  1  3  5  NM_140386.1  CG14106-RA (CG14106), mRNA 
0   0  1  3  3  NM_164861.1  CG4389-RC, transcript variant C (CG4389), mRNA 
0   0  1  3  3  NM_164860.1  CG4389-RB, transcript variant B (CG4389), mRNA 
0   0  1  3  3  NM_135455.2  CG4389-RA, transcript variant A (CG4389), mRNA 
0   0  1  2  4  NM_165223.1  CG6433-RB, transcript variant B (qua), mRNA 
0   0  1  2  4  NM_001014557.1  CG33545-RA (nab), mRNA 
0   0  1  2  3  NM_142396.1  CG7397-RA (CG7397), mRNA 
0   0  1  2  2  NM_168514.1  CG32106-RA (CG32106), mRNA 
0   0  1  2  2  NM_165153.1  CG31817-RA (CG31817), mRNA 
0   0  0  5  10  NM_001042904.1  CG15148-RB, transcript variant B (btv), mRNA 
0   0  0  5  10  NM_001042905.1  CG15148-RC, transcript variant C (btv), mRNA 
0   0  0  5  10  NM_136013.1  CG15148-RA, transcript variant A (btv), mRNA 
0   0  0  5  8  NM_144021.1  CG12710-RA (CG12710), mRNA 
0   0  0  4  11  NM_001031867.1  CG33950-RF, transcript variant F (trol), mRNA 
0   0  0  4  11  NM_001031862.1  CG33950-RC, transcript variant C (trol), mRNA 
0   0  0  4  11  NM_001031864.1  CG33950-RE, transcript variant E (trol), mRNA 
0   0  0  4  11  NM_001031866.1  CG33950-RA, transcript variant A (trol), mRNA 
0   0  0  4  11  NM_001031863.1  CG33950-RD, transcript variant D (trol), mRNA 
0   0  0  4  11  NM_001031865.1  CG33950-RB, transcript variant B (trol), mRNA 

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