NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8841R-1

Symbol CG8841 Full Name CG8841

CG No CG8841 Old CG No CG8841

Synonyms CG8841

Accession No (Link to NCBI) NM_136916.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees female lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTTCGCAAGGCGATTGTGCAG

Primer Seq. 3'

aatctagaggtaccGCTTCCCAAATGTACTCGCAG

Predicted Fragment Size

IR fragment full Seq

0001 NTTCGCAAGG CGATTGTGCA GCTGACGCAG AAGAACCAGA AGATCGACCC CAGCGACGAG 
0061 CAGTTCTGGG AGCAGTTCTG GCAGGGCCAC CAGACGACGC TGGAGGATGT GTTCGCGCTG 
0121 GTCACCTCGA GCGAGATTCG CCAGATACGG AACGAGAATC CAGCCAACCT GGCCACCTTA 
0181 TGCTACAAGG CGGTGGAGAA GCTTGCCCAG GCGGTAGACA GCAGTTGTCG CACGCAGGCG 
0241 GAGCAGCAGT GCGTTTTGAA CTGCGTGCGG CTGCTCGTCC GGTGTCTGCC GTACATTTTC 
0301 GAGGACGAAA AGTGGCGCGA CTTCTTCTGG AGCAGCCTGC CATCGCAGGA CAAGACCATG 
0361 CCGTTGGCTC AGTCACTGCT CAATGCCACC TGCGACCTGC TTTTCTGTCC CGACTTCACG 
0421 GTTACGGCCA CGCGGCGTGC GGGCCCCGAG AAGGCCGAGG AGTTGGCGAA CATCGATAGC 
0481 TGCGAGTACA TTTGGGAAGC

in silico PCR Fragment

0001 TTTCGCAAGG CGATTGTGCA GCTGACGCAG AAGAACCAGA AGATCGACCC CAGCGACGAG 
0061 CAGTTCTGGG AGCAGTTCTG GCAGGGCCAC CAGACGACGC TGGAGGATGT GTTCGCGCTG 
0121 GTCACCTCGA GCGAGATTCG CCAGATACGG AACGAGAATC CAGCCAACCT GGCCACCTTA 
0181 TGCTACAAGG CGGTGGAAAA GCTTGCCCAG GCGGTAGACA GCAGTTGTCG CACGCAGGCG 
0241 GAGCAGCAGT GCGTTTTGAA CTGCGTGCGG CTGCTCGTCC GGTGTCTGCC GTACATTTTC 
0301 GAGGACGAAA AGTGGCGCGA CTTCTTCTGG AGCAGCCTGC CATCGCAGGA CAAGACCATG 
0361 CCGTTGGCTC AGTCACTGCT CAATGCCACC TGCGACCTTC TTTTCTGTCC CGACTTCACG 
0421 GTTACGGCCA CGCGGCGTGC GGGCCCCGAG AAGGCCGAGG AGTTGGCGAA CATCGATAGC 
0481 TGCGAGTACA TTTGGGAAGC

Assemble Data


8841R-1.IR_full        1   TTCGCAAGGCGATTGTGCAGCTGACGCAGAAGAACCAGAAGATCGACCCCAGCGACGAG 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8841R-1.in silico      1   TTCGCAAGGCGATTGTGCAGCTGACGCAGAAGAACCAGAAGATCGACCCCAGCGACGAG 59


8841R-1.IR_full       61  CAGTTCTGGGAGCAGTTCTGGCAGGGCCACCAGACGACGCTGGAGGATGTGTTCGCGCTG 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8841R-1.in silico     61  CAGTTCTGGGAGCAGTTCTGGCAGGGCCACCAGACGACGCTGGAGGATGTGTTCGCGCTG 119


8841R-1.IR_full       121 GTCACCTCGAGCGAGATTCGCCAGATACGGAACGAGAATCCAGCCAACCTGGCCACCTTA 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8841R-1.in silico     121 GTCACCTCGAGCGAGATTCGCCAGATACGGAACGAGAATCCAGCCAACCTGGCCACCTTA 179


8841R-1.IR_full       181 TGCTACAAGGCGGTGGAGAAGCTTGCCCAGGCGGTAGACAGCAGTTGTCGCACGCAGGCG 239
                          ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
8841R-1.in silico     181 TGCTACAAGGCGGTGGAAAAGCTTGCCCAGGCGGTAGACAGCAGTTGTCGCACGCAGGCG 239


8841R-1.IR_full       241 GAGCAGCAGTGCGTTTTGAACTGCGTGCGGCTGCTCGTCCGGTGTCTGCCGTACATTTTC 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8841R-1.in silico     241 GAGCAGCAGTGCGTTTTGAACTGCGTGCGGCTGCTCGTCCGGTGTCTGCCGTACATTTTC 299


8841R-1.IR_full       301 GAGGACGAAAAGTGGCGCGACTTCTTCTGGAGCAGCCTGCCATCGCAGGACAAGACCATG 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8841R-1.in silico     301 GAGGACGAAAAGTGGCGCGACTTCTTCTGGAGCAGCCTGCCATCGCAGGACAAGACCATG 359


8841R-1.IR_full       361 CCGTTGGCTCAGTCACTGCTCAATGCCACCTGCGACCTGCTTTTCTGTCCCGACTTCACG 419
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
8841R-1.in silico     361 CCGTTGGCTCAGTCACTGCTCAATGCCACCTGCGACCTTCTTTTCTGTCCCGACTTCACG 419


8841R-1.IR_full       421 GTTACGGCCACGCGGCGTGCGGGCCCCGAGAAGGCCGAGGAGTTGGCGAACATCGATAGC 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8841R-1.in silico     421 GTTACGGCCACGCGGCGTGCGGGCCCCGAGAAGGCCGAGGAGTTGGCGAACATCGATAGC 479


8841R-1.IR_full       481 TGCGAGTACATTTGGGAAGC 499
                          ||||||||||||||||||||
8841R-1.in silico     481 TGCGAGTACATTTGGGAAGC 499

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_165880.1	CG8841-RC, transcript variant C (CG8841), mRNA
100	482	0	0	0	NM_136916.2	CG8841-RA, transcript variant A (CG8841), mRNA
100	482	0	0	0	NM_165879.1	CG8841-RB, transcript variant B (CG8841), mRNA
0.2	1	2	2	6	NM_136515.2	CG8709-RA (CG8709), mRNA
0	0	4	2	2	NM_143274.1	CG14258-RA (CG14258), mRNA
0	0	3	5	3	NM_143465.1	CG1973-RA (CG1973), mRNA
0	0	3	4	9	NM_205875.1	CG32019-RE, transcript variant E (bt), mRNA
0	0	3	4	9	NM_205876.1	CG32019-RC, transcript variant C (bt), mRNA
0	0	3	4	9	NM_205877.1	CG32019-RD, transcript variant D (bt), mRNA
0	0	3	4	9	NM_166790.2	CG32019-RA, transcript variant A (bt), mRNA
0	0	3	4	5	NM_140948.2	CG6680-RA, transcript variant A (CG6680), mRNA
0	0	3	4	5	NM_168844.1	CG6680-RB, transcript variant B (CG6680), mRNA
0	0	3	3	2	NM_170050.1	CG31365-RA (CG31365), mRNA
0	0	2	6	5	NM_206495.1	CG4898-RK, transcript variant K (Tm1), mRNA
0	0	2	6	4	NM_206494.1	CG4898-RL, transcript variant L (Tm1), mRNA
0	0	2	6	3	NM_169633.2	CG4898-RB, transcript variant B (Tm1), mRNA
0	0	2	6	3	NM_169636.1	CG4898-RJ, transcript variant J (Tm1), mRNA
0	0	2	6	3	NM_169635.1	CG4898-RG, transcript variant G (Tm1), mRNA
0	0	2	6	3	NM_169634.1	CG4898-RD, transcript variant D (Tm1), mRNA
0	0	2	6	3	NM_169638.2	CG4898-RE, transcript variant E (Tm1), mRNA
0	0	2	6	3	NM_169637.2	CG4898-RF, transcript variant F (Tm1), mRNA
0	0	2	5	13	NM_001043010.1	CG9432-RD, transcript variant D (l(2)01289), mRNA
0	0	2	5	13	NM_165485.1	CG9432-RB, transcript variant B (l(2)01289), mRNA
0	0	2	5	3	NM_138228.1	CG13905-RA (CG13905), mRNA
0	0	2	3	6	NM_166047.1	CG8603-RB, transcript variant B (CG8603), mRNA
0	0	2	3	6	NM_137108.2	CG8603-RA, transcript variant A (CG8603), mRNA
0	0	2	2	7	NM_140674.2	CG11915-RA (CG11915), mRNA
0	0	2	2	6	NM_057817.2	CG10637-RA, transcript variant A (Nak), mRNA
0	0	2	2	6	NM_057818.2	CG10637-RB, transcript variant B (Nak), mRNA
0	0	2	2	5	NM_143769.3	CG9432-RA, transcript variant A (l(2)01289), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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