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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8836R-3 |
| Symbol |
Cpr49Ad |
Full Name |
Cuticular protein 49Ad |
| CG No |
CG8836 |
Old CG No |
CG8836 |
| Synonyms |
Cpr49Ad, CG8836 |
| Accession No (Link to NCBI) |
NM_136929.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.
An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.
Zoolog Sci (2016) 33(6) 583-591
[
PubMed ID = 27927092
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGAAGCTGTTCTCCTGCCTTT |
| Primer Seq. 3' |
aatctagaggtaccGTATAGACTACATTGGCAGGG |
| Predicted Fragment Size |
484 |
| IR fragment full Seq |
0001 TGAAGCTGTT CTCCTGCCTT TTCGTGCTCA CCCTGGCTGC CATCGCCTGT GAGGGACAGC
0061 CTCAATACAA TCCCTATCTG CGCAATCCCT ACTACAGGGA TCTCTACTAC AGGAACCGCG
0121 ATCTCTACAA TCTACGACGT TTCTACGATG GCTTCTACAA GAAGTACAAT CCCTACATCG
0181 CCGCTGCCCA GGCTAGAATT GTGGAGCAAA ACAATGATGT GAACTACGGA GCGGGCTCCT
0241 ATTCCTACAA CTATGAGACG GAGAACGGCA TCCATGGCGA GGAGCGCGGA GTGCCAGTGA
0301 ACATTGGAAA CCAGCAGCAA GAGGAGCAAG TTGAGGGTGC TTATTCGTTC ATTACACCCG
0361 AGGGTCTGCG AGTGGGAGTC AAGTACCTGG CTGATGCCAA TGGTTTCCGT CCCGTGATCA
0421 CCTATGATGG TGTGAACTCC GCTTTTTATG CCGGACAACC AGCCCCTGCC AATGTAGTCT
0481 ATAC |
| in silico PCR Fragment |
0001 TGAAGCTGTT CTCCTGCCTT TTCGTGCTCA CCCTGGCTGC CATCGCCTGT GAGGGACAGC
0061 CTCAATACAA TCCCTATCTG CGCAATCCCT ACTACAGGGA TCTCTACTAC AGGAACCGCG
0121 ATCTCTACAA TCTACGACGT TTCTACGATG GCTTCTACAA GAAGTACAAT CCCTACATCG
0181 CCGCTGCCCA GGCTAGAATT GTGGAGCAAA ACAATGATGT GAACTACGGA GCGGGCTCCT
0241 ATTCCTACAA CTATGAGACG GAGAACGGCA TCCATGGCGA GGAGCGCGGA GTGCCAGTGA
0301 ACATTGGAAA CCAGCAGCAG GAGGAGCAAG TTGAGGGTGC TTATTCGTTC ATTACACCCG
0361 AGGGTCTGCG AGTGGGAGTC AAGTACCTGG CTGATGCCAA TGGTTTCCGT CCCGTGATCA
0421 CCTATGATGG TGTGAACTCC GCTTTTTATG CCGGACAGCC AGCCCCTGCC AATGTAGTCT
0481 ATAC |
| Assemble Data |
8836R-3.IR_full 1 TGAAGCTGTTCTCCTGCCTTTTCGTGCTCACCCTGGCTGCCATCGCCTGTGAGGGACAGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-3.in silico 1 TGAAGCTGTTCTCCTGCCTTTTCGTGCTCACCCTGGCTGCCATCGCCTGTGAGGGACAGC 60
8836R-3.IR_full 61 CTCAATACAATCCCTATCTGCGCAATCCCTACTACAGGGATCTCTACTACAGGAACCGCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-3.in silico 61 CTCAATACAATCCCTATCTGCGCAATCCCTACTACAGGGATCTCTACTACAGGAACCGCG 120
8836R-3.IR_full 121 ATCTCTACAATCTACGACGTTTCTACGATGGCTTCTACAAGAAGTACAATCCCTACATCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-3.in silico 121 ATCTCTACAATCTACGACGTTTCTACGATGGCTTCTACAAGAAGTACAATCCCTACATCG 180
8836R-3.IR_full 181 CCGCTGCCCAGGCTAGAATTGTGGAGCAAAACAATGATGTGAACTACGGAGCGGGCTCCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-3.in silico 181 CCGCTGCCCAGGCTAGAATTGTGGAGCAAAACAATGATGTGAACTACGGAGCGGGCTCCT 240
8836R-3.IR_full 241 ATTCCTACAACTATGAGACGGAGAACGGCATCCATGGCGAGGAGCGCGGAGTGCCAGTGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-3.in silico 241 ATTCCTACAACTATGAGACGGAGAACGGCATCCATGGCGAGGAGCGCGGAGTGCCAGTGA 300
8836R-3.IR_full 301 ACATTGGAAACCAGCAGCAAGAGGAGCAAGTTGAGGGTGCTTATTCGTTCATTACACCCG 360
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
8836R-3.in silico 301 ACATTGGAAACCAGCAGCAGGAGGAGCAAGTTGAGGGTGCTTATTCGTTCATTACACCCG 360
8836R-3.IR_full 361 AGGGTCTGCGAGTGGGAGTCAAGTACCTGGCTGATGCCAATGGTTTCCGTCCCGTGATCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8836R-3.in silico 361 AGGGTCTGCGAGTGGGAGTCAAGTACCTGGCTGATGCCAATGGTTTCCGTCCCGTGATCA 420
8836R-3.IR_full 421 CCTATGATGGTGTGAACTCCGCTTTTTATGCCGGACAACCAGCCCCTGCCAATGTAGTCT 480
||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
8836R-3.in silico 421 CCTATGATGGTGTGAACTCCGCTTTTTATGCCGGACAGCCAGCCCCTGCCAATGTAGTCT 480
8836R-3.IR_full 481 ATAC 484
||||
8836R-3.in silico 481 ATAC 484
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
466 |
0 |
0 |
0 |
NM_136929.1 |
CG8836-RA (CG8836), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_140786.1 |
CG18234-RB (CG18234), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_001032052.1 |
CG33715-RE, transcript variant E (Msp-300), mRNA |
| 0 |
0 |
2 |
3 |
22 |
NM_079902.2 |
CG6741-RB, transcript variant B (a), mRNA |
| 0 |
0 |
2 |
3 |
22 |
NM_166514.1 |
CG6741-RA, transcript variant A (a), mRNA |
| 0 |
0 |
2 |
3 |
22 |
NM_001014543.1 |
CG6741-RC, transcript variant C (a), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_078679.2 |
CG6816-RB, transcript variant B (Cyp18a1), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_080253.2 |
CG5067-RA (cic), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_167628.1 |
CG6816-RA, transcript variant A (Cyp18a1), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_137829.1 |
CG10384-RA (CG10384), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_138065.1 |
CG3419-RA, transcript variant A (CG3419), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_206215.1 |
CG3419-RB, transcript variant B (CG3419), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_136503.2 |
CG12769-RA, transcript variant A (CG12769), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_165585.1 |
CG12769-RB, transcript variant B (CG12769), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_137919.1 |
CG13545-RA (CG13545), mRNA |
| 0 |
0 |
1 |
3 |
15 |
NM_134890.2 |
CG17265-RA (CG17265), mRNA |
| 0 |
0 |
1 |
3 |
12 |
NM_166604.1 |
CG9812-RC, transcript variant C (CG9812), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_132017.1 |
CG3160-RA (CG3160), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_134576.2 |
CG1532-RA (CG1532), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_057249.4 |
CG7793-RA (Sos), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_169785.1 |
CG7223-RC, transcript variant C (htl), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_169784.1 |
CG7223-RA, transcript variant A (htl), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_176247.1 |
CG33143-RB, transcript variant B (CG33143), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_176246.1 |
CG33143-RC, transcript variant C (CG33143), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_139925.2 |
CG7979-RA (CG7979), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142421.2 |
CG7218-RA (CG7218), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132182.1 |
CG15326-RA (CG15326), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_141639.1 |
CG16779-RA (CG16779), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_132074.1 |
CG3585-RA (CG3585), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_001043247.1 |
CG6535-RB (tefu), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| +- |
|
| DC |
 |
| +- |
|
| DM |
 |
| +- |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| + |
|
| DC |
|
| + |
|
| DM |
|
| + |
|
| VM |
|
| |
|
| VB |
|
| |
|
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