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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8768R-2 
 Symbol CG8768  Full Name CG8768 
 CG No CG8768  Old CG No CG8768 
 Synonyms CG8768 
 Accession No (Link to NCBI) NM_136969.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGTCGAGGCATGCTCTGATA 
 Primer Seq. 3' 
aatctagaggtaccCCATGACCCACAACAGCGCCA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGTCGAGGC ATGCTCTGAT AGGTGGTGGC ACTGGCTTCA TCGGTCGCAA CCTGGCTAAC 
0061 CATTTGACCA AAAAGGGTTA CGATGTGACG GTGATCTCCC GCATGCCAGG TGCGAAGCGT 
0121 ANTCACCTGG CACGAGCTGG AGAAGAATGG TATTCCCGGA TCCGTAAACG CCGTGGTAAA 
0181 TGCCACGGGC CAGAACACAT TGGATCCAAC GAGACGCTGG ACTCCAGGAT TCCAGCAGAA 
0241 TGTGTGGAAC TCGCGAATCA ACTCCTCAAA AACTTTGGCT CAGGCTATTA AGTCGGCTCC 
0301 CCAGGTCTCA TCTTTCGTGA ATCTATGCGG TGTGAGTCAC TATAAACCCT CGGAGTCCAA 
0361 GGTTTACACC GAGGAGGATC AGGTGCAAGG ATTCGACTAC ATGTCCAGGT TGTGCTTGGC 
0421 CTgGGAGGAG GCTGCACACA CTGGTAGTGA GCAGGATTGT AAAACCACCA TcCTGCGGTG 
0481 TGGCGCTGTT GTGGGTCATg g 
 in silico PCR Fragment 
0001 ATGTCGAGGC ATGCTCTGAT AGGTGGTGGC ACTGGCTTCA TCGGTCGCAA CCTGGCTAAC 
0061 CATTTGACCA AAAAGGGTTA CGATGTGACG GTGATCTCCC GCATGCCAGG TGCGAAGCGT 
0121 A-TCACCTGG CACGAGCTGG AGAAGAATGG TATTCCCGGA TCCGTAAACG CCGTGGTAAA 
0181 TGCCACGGGC CAGAACACAT TGGATCCAAC GAGACGCTGG ACTCCAGGAT TCCAGCAGAA 
0241 TGTGTGGAAC TCGCGAATCA ACTCCTCAAA AACTTTGGCT CAGGCTATTA AGTCGGCTCC 
0301 CCAGGTCTCA TCTTTCGTGA ATCTATGCGG TGTGAGTCAC TATAAACCCT CGGAGTCCAA 
0361 GGTTTACACC GAGGAGGATC AGGTGCAAGG ATTCGACTAC ATGTCCAGGT TGTGCTTGGC 
0421 CTGGGAGGAG GCTGCACACA CTGGTAGTGA GCAGGATTGT AAAACCACCA TCCTGCGGTG 
0481 TGGCGCTGTT GTGGGTCATG G 
 Assemble Data 

8768R-2.IR_full       1   ATGTCGAGGCATGCTCTGATAGGTGGTGGCACTGGCTTCATCGGTCGCAACCTGGCTAAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8768R-2.in silico     1   ATGTCGAGGCATGCTCTGATAGGTGGTGGCACTGGCTTCATCGGTCGCAACCTGGCTAAC 60


8768R-2.IR_full       61  CATTTGACCAAAAAGGGTTACGATGTGACGGTGATCTCCCGCATGCCAGGTGCGAAGCGT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8768R-2.in silico     61  CATTTGACCAAAAAGGGTTACGATGTGACGGTGATCTCCCGCATGCCAGGTGCGAAGCGT 120


8768R-2.IR_full       121 ANTCACCTGGCACGAGCTGGAGAAGAATGGTATTCCCGGATCCGTAAACGCCGTGGTAAA 180
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8768R-2.in silico     121 A-TCACCTGGCACGAGCTGGAGAAGAATGGTATTCCCGGATCCGTAAACGCCGTGGTAAA 180


8768R-2.IR_full       181 TGCCACGGGCCAGAACACATTGGATCCAACGAGACGCTGGACTCCAGGATTCCAGCAGAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8768R-2.in silico     181 TGCCACGGGCCAGAACACATTGGATCCAACGAGACGCTGGACTCCAGGATTCCAGCAGAA 240


8768R-2.IR_full       241 TGTGTGGAACTCGCGAATCAACTCCTCAAAAACTTTGGCTCAGGCTATTAAGTCGGCTCC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8768R-2.in silico     241 TGTGTGGAACTCGCGAATCAACTCCTCAAAAACTTTGGCTCAGGCTATTAAGTCGGCTCC 300


8768R-2.IR_full       301 CCAGGTCTCATCTTTCGTGAATCTATGCGGTGTGAGTCACTATAAACCCTCGGAGTCCAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8768R-2.in silico     301 CCAGGTCTCATCTTTCGTGAATCTATGCGGTGTGAGTCACTATAAACCCTCGGAGTCCAA 360


8768R-2.IR_full       361 GGTTTACACCGAGGAGGATCAGGTGCAAGGATTCGACTACATGTCCAGGTTGTGCTTGGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8768R-2.in silico     361 GGTTTACACCGAGGAGGATCAGGTGCAAGGATTCGACTACATGTCCAGGTTGTGCTTGGC 420


8768R-2.IR_full       421 CTGGGAGGAGGCTGCACACACTGGTAGTGAGCAGGATTGTAAAACCACCATCCTGCGGTG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8768R-2.in silico     421 CTGGGAGGAGGCTGCACACACTGGTAGTGAGCAGGATTGTAAAACCACCATCCTGCGGTG 480


8768R-2.IR_full       481 TGGCGCTGTTGTGGGTCATGG 501
                          |||||||||||||||||||||
8768R-2.in silico     481 TGGCGCTGTTGTGGGTCATGG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  3  NM_136969.2  CG8768-RA (CG8768), mRNA 
0   0  3  3  3  NM_165548.1  CG30495-RA (CG30495), mRNA 
0   0  2  2  3  NM_001014591.1  CG8103-RB, transcript variant B (Mi-2), mRNA 
0   0  2  2  3  NM_140897.2  CG8103-RA, transcript variant A (Mi-2), mRNA 
0   0  1  4  5  NM_132551.2  CG1490-RB (Usp7), mRNA 
0   0  1  4  2  NM_132961.2  CG4955-RA (CG4955), mRNA 
0   0  1  3  7  NM_137203.2  CG8192-RA (CG8192), mRNA 
0   0  1  2  3  NM_143059.1  CG13646-RA (CG13646), mRNA 
0   0  1  2  2  NM_135247.2  CG9138-RA (SP1070), mRNA 
0   0  0  7  8  NM_140022.1  CG5087-RA (CG5087), mRNA 
0   0  0  6  3  NM_078666.2  CG8146-RA (Socs16D), mRNA 
0   0  0  5  3  NM_206551.1  CG5320-RF, transcript variant F (Gdh), mRNA 
0   0  0  5  3  NM_079746.4  CG5320-RA, transcript variant A (Gdh), mRNA 
0   0  0  5  3  NM_206550.1  CG5320-RE, transcript variant E (Gdh), mRNA 
0   0  0  5  3  NM_170101.1  CG5320-RB, transcript variant B (Gdh), mRNA 
0   0  0  4  10  NM_165485.1  CG9432-RB, transcript variant B (l(2)01289), mRNA 
0   0  0  4  10  NM_001043010.1  CG9432-RD, transcript variant D (l(2)01289), mRNA 
0   0  0  4  7  NM_057739.3  CG3969-RB, transcript variant B (PR2), mRNA 
0   0  0  4  7  NM_057738.3  CG3969-RA, transcript variant A (PR2), mRNA 
0   0  0  4  5  NM_137419.2  CG6385-RA (CG6385), mRNA 
0   0  0  4  4  NM_139399.1  CG13921-RA, transcript variant A (CG13921), mRNA 
0   0  0  4  4  NM_206235.1  CG13921-RB, transcript variant B (CG13921), mRNA 
0   0  0  4  4  NM_141220.1  CG9783-RA (CG9783), mRNA 
0   0  0  4  3  NM_001032052.1  CG33715-RE, transcript variant E (Msp-300), mRNA 
0   0  0  4  2  NM_136391.2  CG9436-RA (CG9436), mRNA 
0   0  0  4  2  NM_170615.1  CG8732-RA, transcript variant A (l(2)44DEa), mRNA 
0   0  0  4  2  NM_001014617.1  CG7583-RE, transcript variant E (CtBP), mRNA 
0   0  0  4  2  NM_001014511.1  CG8732-RE, transcript variant E (l(2)44DEa), mRNA 
0   0  0  4  2  NM_142172.2  CG6654-RA (CG6654), mRNA 
0   0  0  4  2  NM_143777.2  CG8732-RB, transcript variant B (l(2)44DEa), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
+ 
DM
++ 
VM
++ 
VB
++ 
Dcompartment   Penetrance
+     

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