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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8680R-1 |
| Symbol |
CG8680 |
Full Name |
CG8680 |
| CG No |
CG8680 |
Old CG No |
CG8680 |
| Synonyms |
CG8680 |
| Accession No (Link to NCBI) |
NM_135065.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGCCAGCAAGCAATTGGTAA |
| Primer Seq. 3' |
aatctagaggtaccGGTCATCTTTCTTGACGAAGC |
| Predicted Fragment Size |
369 |
| IR fragment full Seq |
0001 TGGCCAGCAA GCAATTGGTA AACAATTTGT CCAAACTCGG CCTTCCGCGA CAGAATTGGA
0061 TGTCCCCACT GGCCAGCGTT CGCCACTNCA AGCTGCCGCG GCGACATCGA GAAGGTCACA
0121 CACACCGGAC AAGTGTTCGA TAAGGANGGA CTACCGCAAT GCCCGGTTCG TGAACGCCAA
0181 GCGGTATGTG AACGAGAACT GGGGCATCAA GCTCATCGAG GAGGTGCCAC CCAAGGAGTG
0241 CACCGAACGA GTTGTCTTCT GCGACGGCGG CGATGGTCCT TTGGGTCATC CCAAGGTGTA
0301 CATCAACCTG GACAAACCCG GAAATCACAT CTGCGGCTAT CTGCGGCCTG CGCTTCGTCA
0361 AGAAAGATGA CC |
| in silico PCR Fragment |
0001 TGGCCAGCAA GCAATTGGTA AACAATTTGT CCAAACTCGG CCTTCCGCGA CAGAATTGGA
0061 TGTCCCCACT GGCCAGCGTT CGCCACT-CC AGCTGCCGCG GCGACATCGA GAAGGTTACA
0121 CACACCGGAC AAGTGTTCGA TAAGGA-GGA CTACCGCAAT GCCCGGTTCG TGAACGCCAA
0181 GCGGTATGTG AACGAGAACT GGGGCATCAA GCTCATCGAG GAGGTGCCAC CCAAGGAGTG
0241 CACCGAACGA GTTGTCTTCT GCGACGGCGG CGATGGTCCT TTGGGTCATC CCAAAGTGTA
0301 CATCAACCTG GACAAACCCG GAAATCACAT CTGCGGCTA- CTGCGGCCTG CGCTTCGTCA
0361 AGAAAGATGA CC |
| Assemble Data |
8680R-1.IR_full 1 TGGCCAGCAAGCAATTGGTAAACAATTTGTCCAAACTCGGCCTTCCGCGACAGAATTGGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8680R-1.in silico 1 TGGCCAGCAAGCAATTGGTAAACAATTTGTCCAAACTCGGCCTTCCGCGACAGAATTGGA 60
8680R-1.IR_full 61 TGTCCCCACTGGCCAGCGTTCGCCACTNCAAGCTGCCGCGGCGACATCGAGAAGGTCACA 120
||||||||||||||||||||||||||| | |||||||||||||||||||||||||| |||
8680R-1.in silico 61 TGTCCCCACTGGCCAGCGTTCGCCACT-CCAGCTGCCGCGGCGACATCGAGAAGGTTACA 120
8680R-1.IR_full 121 CACACCGGACAAGTGTTCGATAAGGANGGACTACCGCAATGCCCGGTTCGTGAACGCCAA 180
|||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
8680R-1.in silico 121 CACACCGGACAAGTGTTCGATAAGGA-GGACTACCGCAATGCCCGGTTCGTGAACGCCAA 180
8680R-1.IR_full 181 GCGGTATGTGAACGAGAACTGGGGCATCAAGCTCATCGAGGAGGTGCCACCCAAGGAGTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8680R-1.in silico 181 GCGGTATGTGAACGAGAACTGGGGCATCAAGCTCATCGAGGAGGTGCCACCCAAGGAGTG 240
8680R-1.IR_full 241 CACCGAACGAGTTGTCTTCTGCGACGGCGGCGATGGTCCTTTGGGTCATCCCAAGGTGTA 300
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
8680R-1.in silico 241 CACCGAACGAGTTGTCTTCTGCGACGGCGGCGATGGTCCTTTGGGTCATCCCAAAGTGTA 300
8680R-1.IR_full 301 CATCAACCTGGACAAACCCGGAAATCACATCTGCGGCTATCTGCGGCCTGCGCTTCGTCA 360
||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
8680R-1.in silico 301 CATCAACCTGGACAAACCCGGAAATCACATCTGCGGCTA-CTGCGGCCTGCGCTTCGTCA 360
8680R-1.IR_full 361 AGAAAGATGACC 372
||||||||||||
8680R-1.in silico 361 AGAAAGATGACC 372
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
351 |
0 |
0 |
0 |
NM_135065.2 |
CG8680-RA (CG8680), mRNA |
| 0.28 |
1 |
3 |
2 |
2 |
NM_139716.2 |
CG10596-RB, transcript variant B (Msr-110), mRNA |
| 0.28 |
1 |
3 |
2 |
2 |
NM_168123.1 |
CG10596-RA, transcript variant A (Msr-110), mRNA |
| 0.28 |
1 |
3 |
2 |
2 |
NM_168124.1 |
CG10596-RC, transcript variant C (Msr-110), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_132432.1 |
CG2186-RA (CG2186), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_001043332.1 |
CG2005-RE, transcript variant E (Ptp99A), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_206579.1 |
CG2005-RC, transcript variant C (Ptp99A), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_170409.1 |
CG2005-RA, transcript variant A (Ptp99A), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_001014683.1 |
CG2005-RD, transcript variant D (Ptp99A), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_057468.2 |
CG2005-RB, transcript variant B (Ptp99A), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142697.2 |
CG5862-RA (CG5862), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137943.2 |
CG3493-RA (CG3493), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_132896.1 |
CG9981-RA (CG9981), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143497.2 |
CG7896-RA (CG7896), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057819.3 |
CG9258-RA (nrv1), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_137707.2 |
CG30388-RA (Magi), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_137653.2 |
CG11175-RA (CG11175), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_168310.1 |
CG5263-RC, transcript variant C (smg), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_079263.2 |
CG5263-RA, transcript variant A (smg), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_168309.1 |
CG5263-RB, transcript variant B (smg), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_057961.4 |
CG3771-RA (a6), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_132969.2 |
CG8918-RA (CG8918), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_168354.1 |
CG8336-RB, transcript variant B (CG8336), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_140081.2 |
CG8336-RA, transcript variant A (CG8336), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_168355.1 |
CG8336-RC, transcript variant C (CG8336), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_079817.2 |
CG1842-RA (Dhc98D), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_001032407.1 |
CG33956-RA, transcript variant A (kay), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_165802.1 |
CG30015-RA, transcript variant A (CG30015), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_136777.2 |
CG30015-RB, transcript variant B (CG30015), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_132390.1 |
CG15306-RA (CG15306), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| ++ |
|
| VM |
 |
| ++ |
|
| VB |
 |
| ++ |
|
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