NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8638R-1

Symbol Cpr65Eb Full Name Cuticular protein 65Eb

CG No CG8638 Old CG No CG8638

Synonyms BcDNA:RE28679, Cpr65Eb, CG8638

Accession No (Link to NCBI) NM_139819.2

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.
An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.
Zoolog Sci (2016) 33(6) 583-591 [ PubMed ID = 27927092 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCATGCCGAGATCCGGAGCTTT

Primer Seq. 3'

aatctagaggtaccCTGTCGTAGGGCAGGACCTGA

Predicted Fragment Size

IR fragment full Seq

0001 CATGCCGAGA TCCGGAGCTT TGTCAACGAG CTGAAGCAGG AGGATGGCAT CTACAATTAC 
0061 CAGTTCGAGA CGTCCAACGG GATTGCGCAG CAGGAGCAGG GAGTGGGTGG TTACTACGCT 
0121 AGCGGCTCAT CACAGTACTA CACACCCGAG GGCCAGCTCA TCCAGTTGAC CTACACGGCA 
0181 GATGAGAACG GATTCCAGCC GCAGGGCGAG CACCTGCCCA CGCCCCATCC CATTCCCGAG 
0241 GCAATCCTGA AGTCGCTGGA GTACAATCGC AACCACCCGG AGGAGGATGG GGAGTACGAG 
0301 CACCACGACC ATCACCATGA CCACCATGAG CATCACCAGA GCCATGGTCA GGAGCAGGGC 
0361 AAGCAATATC TGCAGCCAGC CGGTGCTCTG CTCGCACCTG CGTCATCGCC AGTGTTGTCT 
0421 GTGGGTCAGG TCCTGCCNTA CGACAG

in silico PCR Fragment

0001 CATGCCGAGA TCCGGAGCTT TGTCAACGAA CTGAAGCAGG AGGATGGCAT CTACAATTAC 
0061 CAGTTCGAGA CGTCCAACGG GATTGCGCAG CAGGAGCAGG GAGTGGGTGG TTACTACGCT 
0121 AGCGGCTCAT CACAGTACTA CACACCCGAG GGCCAGCTCA TCCAGTTGAC CTACACGGCA 
0181 GATGAGAACG GATTCCAGCC GCAGGGCGAG CACCTGCCCA CGCCCCATCC CATTCCCGAG 
0241 GCAATCCTGA AGTCGCTGGA GTACAATCGC AACCACCCGG AGGAGGATGG GGAGTACGAG 
0301 CACCACGACC ATCACCATGA CCACCATGAG CATCACCAGA GCCATGGTCA GGAGCAGGGC 
0361 AAGCAGTATC TGCAGCCAGC CGGTGCTCTG CTCGCACCTG CGTCATCGCC AGTGTTGTCT 
0421 GTGGGTCAGG TCCTGCCCTA CGACAG

Assemble Data


8638R-1.IR_full       1   CATGCCGAGATCCGGAGCTTTGTCAACGAGCTGAAGCAGGAGGATGGCATCTACAATTAC 60
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
8638R-1.in silico     1   CATGCCGAGATCCGGAGCTTTGTCAACGAACTGAAGCAGGAGGATGGCATCTACAATTAC 60


8638R-1.IR_full       61  CAGTTCGAGACGTCCAACGGGATTGCGCAGCAGGAGCAGGGAGTGGGTGGTTACTACGCT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8638R-1.in silico     61  CAGTTCGAGACGTCCAACGGGATTGCGCAGCAGGAGCAGGGAGTGGGTGGTTACTACGCT 120


8638R-1.IR_full       121 AGCGGCTCATCACAGTACTACACACCCGAGGGCCAGCTCATCCAGTTGACCTACACGGCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8638R-1.in silico     121 AGCGGCTCATCACAGTACTACACACCCGAGGGCCAGCTCATCCAGTTGACCTACACGGCA 180


8638R-1.IR_full       181 GATGAGAACGGATTCCAGCCGCAGGGCGAGCACCTGCCCACGCCCCATCCCATTCCCGAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8638R-1.in silico     181 GATGAGAACGGATTCCAGCCGCAGGGCGAGCACCTGCCCACGCCCCATCCCATTCCCGAG 240


8638R-1.IR_full       241 GCAATCCTGAAGTCGCTGGAGTACAATCGCAACCACCCGGAGGAGGATGGGGAGTACGAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8638R-1.in silico     241 GCAATCCTGAAGTCGCTGGAGTACAATCGCAACCACCCGGAGGAGGATGGGGAGTACGAG 300


8638R-1.IR_full       301 CACCACGACCATCACCATGACCACCATGAGCATCACCAGAGCCATGGTCAGGAGCAGGGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8638R-1.in silico     301 CACCACGACCATCACCATGACCACCATGAGCATCACCAGAGCCATGGTCAGGAGCAGGGC 360


8638R-1.IR_full       361 AAGCAATATCTGCAGCCAGCCGGTGCTCTGCTCGCACCTGCGTCATCGCCAGTGTTGTCT 420
                          ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
8638R-1.in silico     361 AAGCAGTATCTGCAGCCAGCCGGTGCTCTGCTCGCACCTGCGTCATCGCCAGTGTTGTCT 420


8638R-1.IR_full       421 GTGGGTCAGGTCCTGCCNTACGACAG 446
                          ||||||||||||||||| ||||||||
8638R-1.in silico     421 GTGGGTCAGGTCCTGCCCTACGACAG 446

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	428	0	0	4	NM_139819.2	CG8638-RA (CG8638), mRNA
1.86	8	13	20	26	NM_139820.1	CG8634-RA (CG8634), mRNA
1.4	6	3	6	13	NM_130551.1	CG14770-RA (CG14770), mRNA
1.16	5	15	6	6	NM_139774.1	CG12330-RA (CG12330), mRNA
0.7	3	9	4	8	NM_136932.1	CG8511-RA (CG8511), mRNA
0.46	2	10	3	8	NM_144403.1	CG18777-RA (CG18777), mRNA
0.46	2	9	3	7	NM_176290.1	CG10529-RA (Lcp65Ae), mRNA
0.46	2	5	3	2	NM_165194.2	CG31991-RB, transcript variant B (mdy), mRNA
0.46	2	4	4	2	NM_206002.1	CG31991-RD, transcript variant D (mdy), mRNA
0.46	2	3	3	3	NM_001042896.1	CG16863-RB, transcript variant B (CG16863), mRNA
0.46	2	3	3	3	NM_135830.3	CG16863-RA, transcript variant A (CG16863), mRNA
0.46	2	2	5	12	NM_142397.1	CG14318-RA (CG14318), mRNA
0.46	2	2	4	3	NM_165195.2	CG31991-RC, transcript variant C (mdy), mRNA
0.46	2	2	3	2	NM_135969.3	CG31991-RA, transcript variant A (mdy), mRNA
0.23	1	6	2	2	NM_142113.1	CG14854-RA (CG14854), mRNA
0	0	16	9	10	NM_165891.1	CG30045-RA (CG30045), mRNA
0	0	13	11	5	NM_136930.3	CG8505-RA (CG8505), mRNA
0	0	6	7	2	NM_057924.2	CG10534-RA (Lcp65Ag2), mRNA
0	0	6	7	2	NM_057925.2	CG10530-RA (Lcp65Ag1), mRNA
0	0	5	12	7	NM_057932.1	CG7287-RA (Lcp65Aa), mRNA
0	0	5	6	6	NR_001423.1	CG7287-RA (Lcp65Aa), mRNA, mRNA
0	0	3	13	20	NM_001043144.1	CG4998-RB, transcript variant B (CG4998), mRNA
0	0	3	3	3	NM_140783.2	CG7285-RA (star1), mRNA
0	0	3	2	5	NM_143372.1	CG9995-RA (htt), mRNA
0	0	3	2	3	NM_078632.2	CG17336-RB, transcript variant B (Lcch3), mRNA
0	0	3	2	3	NM_206746.1	CG17336-RC, transcript variant C (Lcch3), mRNA
0	0	3	2	3	NM_167475.1	CG17336-RA, transcript variant A (Lcch3), mRNA
0	0	2	14	13	NM_168158.1	CG32405-RA (CG32405), mRNA
0	0	2	12	6	NM_176291.1	CG18773-RA (Lcp65Ab2), mRNA
0	0	2	12	6	NM_080075.2	CG32400-RA (Lcp65Ab1), mRNA

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