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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8631R-3 |
| Symbol |
msl-3 |
Full Name |
male-specific lethal 3 |
| CG No |
CG8631 |
Old CG No |
CG8631 |
| Synonyms |
msl3, MSL3, Msl3, msl, MSL-3, MSL, DmMsl-3, mls3, mle3, mle(3)132, CG8631, msl-3, Msl 1-3 |
| Accession No (Link to NCBI) |
NM_079227.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCTAAGGGACGAGACACCGCT |
| Primer Seq. 3' |
aatctagaggtaccTTAATCGGCCATCTCCGGACG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCTAAGGGAC GAGACACCGC TCTTTCACAA GGGCGAGATA GTCCTTTGCT ACGAGCCGGA
0061 TAAATCGAAG GCCCGTGTTC TTTACACCAG CAAGGTGCTA AACGTCTTTG AGCGCCGGAA
0121 CGAGCATGGC CTGAGGTTCT ACGAGTACAA GATTCACTTC CAGGGCTGGC GTCCATCCTA
0181 CGATCGCTGT GTGCGCGCCA CCGTTCTATT GAAAGACACC GAGGAGAATC GCCAGTTGCA
0241 AAGGGAGCTG GCCGAAGCTG CCAAGCTGCA AATTCGTGGT GACTACTCCT ACAANGGGCA
0301 CACCCGATAA NNCATCGGCC AAGAAGAAAC GCGGCGGAAA AGCGGCCCAC GTTGAGGAGC
0361 CAATAGTGGT TCCAATGGAC ACGGGACATC TTGAgGCGGA GCACGAAATG GCGCCCACTC
0421 CACGAGCAGC GGGCAACCGG ACGCGGGACA ACAGCGGTGG CAAGCGAAAG GAAAAACCCN
0481 CGTCCGGAGA TGGCCGATTA A |
| in silico PCR Fragment |
0001 GCTAAGGGAC GAGACACCGC TCTTTCACAA GGGCGAGATA GTCCTTTGCT ACGAGCCGGA
0061 TAAATCGAAG GCCCGTGTTC TTTACACCAG CAAGGTGCTA AACGTCTTTG AGCGCCGGAA
0121 CGAGCATGGC CTGAGGTTCT ACGAGTACAA GATTCACTTC CAGGGCTGGC GTCCATCCTA
0181 CGATCGCTGT GTGCGCGCCA CCGTTCTATT GAAAGACACC GAGGAGAATC GCCAGTTGCA
0241 AAGGGAGCTG GCCGAAGCTG CCAAGCTGCA AATTCGTGGT GACTACTCCT ACAA-GGGCA
0301 CACCCGATAA GCCATCGGCC AAGAAGAAAC GCGGCGGAAA AGCGGCCCAC GTTGAGGAGC
0361 CAATAGTGGT TCCAATGGAC ACGGGACATC TTGAGGCGGA GCACGAAATG GCGCCCACTC
0421 CACGAGCAGC GGGCAACCGG ACGCGGGACA ACAGCGGTGG CAAGCGAAAG GAAAAACCCC
0481 CGTCCGGAGA TGGCCGATTA A |
| Assemble Data |
8631R-3.IR_full 1 GCTAAGGGACGAGACACCGCTCTTTCACAAGGGCGAGATAGTCCTTTGCTACGAGCCGGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8631R-3.in silico 1 GCTAAGGGACGAGACACCGCTCTTTCACAAGGGCGAGATAGTCCTTTGCTACGAGCCGGA 60
8631R-3.IR_full 61 TAAATCGAAGGCCCGTGTTCTTTACACCAGCAAGGTGCTAAACGTCTTTGAGCGCCGGAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8631R-3.in silico 61 TAAATCGAAGGCCCGTGTTCTTTACACCAGCAAGGTGCTAAACGTCTTTGAGCGCCGGAA 120
8631R-3.IR_full 121 CGAGCATGGCCTGAGGTTCTACGAGTACAAGATTCACTTCCAGGGCTGGCGTCCATCCTA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8631R-3.in silico 121 CGAGCATGGCCTGAGGTTCTACGAGTACAAGATTCACTTCCAGGGCTGGCGTCCATCCTA 180
8631R-3.IR_full 181 CGATCGCTGTGTGCGCGCCACCGTTCTATTGAAAGACACCGAGGAGAATCGCCAGTTGCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8631R-3.in silico 181 CGATCGCTGTGTGCGCGCCACCGTTCTATTGAAAGACACCGAGGAGAATCGCCAGTTGCA 240
8631R-3.IR_full 241 AAGGGAGCTGGCCGAAGCTGCCAAGCTGCAAATTCGTGGTGACTACTCCTACAANGGGCA 300
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
8631R-3.in silico 241 AAGGGAGCTGGCCGAAGCTGCCAAGCTGCAAATTCGTGGTGACTACTCCTACAA-GGGCA 300
8631R-3.IR_full 301 CACCCGATAANNCATCGGCCAAGAAGAAACGCGGCGGAAAAGCGGCCCACGTTGAGGAGC 360
|||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
8631R-3.in silico 301 CACCCGATAAGCCATCGGCCAAGAAGAAACGCGGCGGAAAAGCGGCCCACGTTGAGGAGC 360
8631R-3.IR_full 361 CAATAGTGGTTCCAATGGACACGGGACATCTTGAGGCGGAGCACGAAATGGCGCCCACTC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8631R-3.in silico 361 CAATAGTGGTTCCAATGGACACGGGACATCTTGAGGCGGAGCACGAAATGGCGCCCACTC 420
8631R-3.IR_full 421 CACGAGCAGCGGGCAACCGGACGCGGGACAACAGCGGTGGCAAGCGAAAGGAAAAACCCN 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8631R-3.in silico 421 CACGAGCAGCGGGCAACCGGACGCGGGACAACAGCGGTGGCAAGCGAAAGGAAAAACCCC 480
8631R-3.IR_full 481 CGTCCGGAGATGGCCGATTAA 501
|||||||||||||||||||||
8631R-3.in silico 481 CGTCCGGAGATGGCCGATTAA 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
599 |
6 |
6 |
8 |
NM_168191.1 |
CG8631-RB, transcript variant B (msl-3), mRNA |
| 78.13 |
468 |
2 |
2 |
2 |
NM_079227.2 |
CG8631-RA, transcript variant A (msl-3), mRNA |
| 0.83 |
5 |
12 |
31 |
46 |
NM_132109.1 |
CG3198-RA (CG3198), mRNA |
| 0.66 |
4 |
4 |
31 |
71 |
NM_133108.2 |
CG32542-RA (CG32542), mRNA |
| 0.5 |
3 |
14 |
21 |
41 |
NM_166432.1 |
CG9480-RB, transcript variant B (Glycogenin), mRNA |
| 0.5 |
3 |
12 |
19 |
39 |
NM_079180.2 |
CG10579-RD, transcript variant D (Eip63E), mRNA |
| 0.5 |
3 |
12 |
19 |
39 |
NM_001043116.1 |
CG10579-RF, transcript variant F (Eip63E), mRNA |
| 0.5 |
3 |
8 |
15 |
20 |
NM_165665.1 |
CG8029-RA, transcript variant A (CG8029), mRNA |
| 0.5 |
3 |
8 |
15 |
20 |
NM_136626.4 |
CG8029-RB, transcript variant B (CG8029), mRNA |
| 0.33 |
2 |
7 |
17 |
31 |
NM_166076.1 |
CG12424-RB, transcript variant B (CG12424), mRNA |
| 0.33 |
2 |
7 |
14 |
24 |
NM_079871.2 |
CG1775-RA, transcript variant A (Med), mRNA |
| 0.33 |
2 |
7 |
14 |
24 |
NM_170559.1 |
CG1775-RB, transcript variant B (Med), mRNA |
| 0.33 |
2 |
7 |
11 |
13 |
NM_001038920.1 |
CG33991-RC, transcript variant C (nuf), mRNA |
| 0.33 |
2 |
7 |
11 |
13 |
NM_001038922.1 |
CG33991-RA, transcript variant A (nuf), mRNA |
| 0.33 |
2 |
6 |
24 |
42 |
NM_134714.3 |
CG4341-RA (CG4341), mRNA |
| 0.33 |
2 |
6 |
14 |
43 |
NM_206091.1 |
CG7734-RD, transcript variant D (shn), mRNA |
| 0.33 |
2 |
5 |
11 |
14 |
NM_080083.3 |
CG7004-RA, transcript variant A (fwd), mRNA |
| 0.33 |
2 |
5 |
9 |
10 |
NM_141625.2 |
CG8866-RB, transcript variant B (CG8866), mRNA |
| 0.33 |
2 |
5 |
9 |
10 |
NM_169265.1 |
CG8866-RA, transcript variant A (CG8866), mRNA |
| 0.33 |
2 |
3 |
2 |
3 |
NM_168112.1 |
CG32423-RB, transcript variant B (alan-shepard), mRNA |
| 0.33 |
2 |
2 |
4 |
10 |
NM_168399.1 |
CG32057-RC, transcript variant C (dpr10), mRNA |
| 0.16 |
1 |
9 |
19 |
33 |
NM_132059.2 |
CG4766-RA (CG4766), mRNA |
| 0.16 |
1 |
7 |
22 |
51 |
NM_166867.1 |
CG11642-RB, transcript variant B (TRAM), mRNA |
| 0.16 |
1 |
7 |
21 |
30 |
NM_166866.1 |
CG11642-RA, transcript variant A (TRAM), mRNA |
| 0.16 |
1 |
7 |
14 |
13 |
NM_001043286.1 |
CG6383-RB, transcript variant B (crb), mRNA |
| 0.16 |
1 |
7 |
14 |
13 |
NM_079756.2 |
CG6383-RA, transcript variant A (crb), mRNA |
| 0.16 |
1 |
7 |
6 |
17 |
NM_134490.2 |
CG12238-RA (l(1)G0084), mRNA |
| 0.16 |
1 |
5 |
20 |
41 |
NM_132625.1 |
CG4351-RA (CG4351), mRNA |
| 0.16 |
1 |
4 |
12 |
23 |
NM_142570.2 |
CG7535-RA, transcript variant A (GluClalpha), mRNA |
| 0.16 |
1 |
4 |
12 |
23 |
NM_001014641.1 |
CG7535-RC, transcript variant C (GluClalpha), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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