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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8625R-2 |
| Symbol |
Iswi |
Full Name |
Imitation SWI |
| CG No |
CG8625 |
Old CG No |
CG8625 |
| Synonyms |
NURF, ISWI, CG8625, ACF, iswi, p140/ISWI, p140, dNURF, CHRAC, dISWI, dCHRAC, SWI, ISW, NURF-140, anon-EP1279744.124, Nurf-140, Iswi |
| Accession No (Link to NCBI) |
NM_078995.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTACAGCTGCCGTGGAGGCAAC |
| Primer Seq. 3' |
aatctagaggtaccGCTTGAGGTAACCCAGCAGAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TACAGCTGcC GTGGAGGCAA CCGAAGAGAA CTCGAACGAG ACGACTTCAG ATGCGGCCAC
0061 CAGTTCATCC GGTGAAAAGG AGGCTGAGTT CGACAACAAA ATCGAGGCTG ATCGCAGTAG
0121 GcGCTTTGAT TTCCTGCTAA AGCAGACGGA GATATTCACC CACTTCATGA CTAACAGCGC
0181 TAAGAGTCCC ACGAAGCCTA AGGGTAGACC CAAGAAGATC AAAGACAAGG ACAAGGAAAA
0241 GGATGTGGCC GATCATCGTC ATCGCAAGAC AGAGCAGTGA GGAGGATGAG GAGTTGCTGG
0301 CGGAAGACTC GGCCACCAAG GAGATCTTTC GCTTCGATGC CTCACCCGCC TACATCAAAA
0361 GTGGAGAGAT GCGTGACTAC CAGATTCGCG GTCTTAACTG GATGATTTCG CTTTACGAAA
0421 ATGGTATCAA TGGAATTCTG GCCGATGAAA TGGGTCTAGG AAAGACCCTG CAGACCATAT
0481 CTCTGCTGGG TTACCTCAAG C |
| in silico PCR Fragment |
0001 TACAGCTGCC GTGGAGGCAA CCGAAGAGAA CTCGAACGAG ACGACTTCAG ATGCGGCCAC
0061 CAGTTCATCC GGTGAAAAGG AGGCTGAGTT CGACAACAAA ATCGAGGCTG ATCGCAGTAG
0121 GCGCTTTGAT TTCCTGCTAA AGCAGACGGA GATATTCACC CACTTCATGA CTAACAGCGC
0181 TAAGAGTCCC ACGAAGCCTA AGGGTAGACC CAAGAAGATC AAAGACAAGG ACAAGGAAAA
0241 GGATGTGGCC GATCATCGTC ATCGCAAGAC AGAGCAG-GA GGAGGATGAG GAGTTGCTGG
0301 CGGAAGACTC GGCCACCAAG GAGATCTTTC GCTTCGATGC CTCACCCGCC TACATCAAAA
0361 GTGGAGAGAT GCGTGACTAC CAGATTCGCG GTCTTAACTG GATGATTTCG CTTTACGAAA
0421 ATGGTATCAA TGGAATTCTG GCCGATGAAA TGGGTCTAGG AAAGACCCTG CAGACCATAT
0481 CTCTGCTGGG TTACCTCAAG C |
| Assemble Data |
8625R-2.IR_full 1 TACAGCTGCCGTGGAGGCAACCGAAGAGAACTCGAACGAGACGACTTCAGATGCGGCCAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8625R-2.in silico 1 TACAGCTGCCGTGGAGGCAACCGAAGAGAACTCGAACGAGACGACTTCAGATGCGGCCAC 60
8625R-2.IR_full 61 CAGTTCATCCGGTGAAAAGGAGGCTGAGTTCGACAACAAAATCGAGGCTGATCGCAGTAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8625R-2.in silico 61 CAGTTCATCCGGTGAAAAGGAGGCTGAGTTCGACAACAAAATCGAGGCTGATCGCAGTAG 120
8625R-2.IR_full 121 GCGCTTTGATTTCCTGCTAAAGCAGACGGAGATATTCACCCACTTCATGACTAACAGCGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8625R-2.in silico 121 GCGCTTTGATTTCCTGCTAAAGCAGACGGAGATATTCACCCACTTCATGACTAACAGCGC 180
8625R-2.IR_full 181 TAAGAGTCCCACGAAGCCTAAGGGTAGACCCAAGAAGATCAAAGACAAGGACAAGGAAAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8625R-2.in silico 181 TAAGAGTCCCACGAAGCCTAAGGGTAGACCCAAGAAGATCAAAGACAAGGACAAGGAAAA 240
8625R-2.IR_full 241 GGATGTGGCCGATCATCGTCATCGCAAGACAGAGCAGTGAGGAGGATGAGGAGTTGCTGG 300
||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
8625R-2.in silico 241 GGATGTGGCCGATCATCGTCATCGCAAGACAGAGCAG-GAGGAGGATGAGGAGTTGCTGG 300
8625R-2.IR_full 301 CGGAAGACTCGGCCACCAAGGAGATCTTTCGCTTCGATGCCTCACCCGCCTACATCAAAA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8625R-2.in silico 301 CGGAAGACTCGGCCACCAAGGAGATCTTTCGCTTCGATGCCTCACCCGCCTACATCAAAA 360
8625R-2.IR_full 361 GTGGAGAGATGCGTGACTACCAGATTCGCGGTCTTAACTGGATGATTTCGCTTTACGAAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8625R-2.in silico 361 GTGGAGAGATGCGTGACTACCAGATTCGCGGTCTTAACTGGATGATTTCGCTTTACGAAA 420
8625R-2.IR_full 421 ATGGTATCAATGGAATTCTGGCCGATGAAATGGGTCTAGGAAAGACCCTGCAGACCATAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8625R-2.in silico 421 ATGGTATCAATGGAATTCTGGCCGATGAAATGGGTCTAGGAAAGACCCTGCAGACCATAT 480
8625R-2.IR_full 481 CTCTGCTGGGTTACCTCAAGC 501
|||||||||||||||||||||
8625R-2.in silico 481 CTCTGCTGGGTTACCTCAAGC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_078995.1 |
CG8625-RA, transcript variant A (Iswi), mRNA |
| 100 |
482 |
0 |
0 |
4 |
NM_165930.1 |
CG8625-RB, transcript variant B (Iswi), mRNA |
| 100 |
482 |
0 |
0 |
4 |
NM_165931.1 |
CG8625-RC, transcript variant C (Iswi), mRNA |
| 0.2 |
1 |
3 |
2 |
2 |
NM_166109.1 |
CG8205-RB, transcript variant B (fus), mRNA |
| 0.2 |
1 |
3 |
2 |
2 |
NM_166107.1 |
CG8205-RE, transcript variant E (fus), mRNA |
| 0.2 |
1 |
3 |
2 |
2 |
NM_166108.1 |
CG8205-RF, transcript variant F (fus), mRNA |
| 0.2 |
1 |
3 |
2 |
2 |
NM_079952.2 |
CG8205-RD, transcript variant D (fus), mRNA |
| 0 |
0 |
14 |
32 |
45 |
NM_132578.1 |
CG11146-RA (CG11146), mRNA |
| 0 |
0 |
8 |
8 |
15 |
NM_079522.2 |
CG10281-RA (TfIIFalpha), mRNA |
| 0 |
0 |
6 |
6 |
24 |
NM_136942.2 |
CG8545-RA (CG8545), mRNA |
| 0 |
0 |
5 |
10 |
25 |
NM_143377.2 |
CG1646-RA, transcript variant A (CG1646), mRNA |
| 0 |
0 |
5 |
10 |
25 |
NM_176576.1 |
CG1646-RC, transcript variant C (CG1646), mRNA |
| 0 |
0 |
5 |
10 |
25 |
NM_176577.1 |
CG1646-RD, transcript variant D (CG1646), mRNA |
| 0 |
0 |
5 |
10 |
25 |
NM_170377.1 |
CG1646-RB, transcript variant B (CG1646), mRNA |
| 0 |
0 |
5 |
2 |
9 |
NM_176447.1 |
CG33208-RF, transcript variant F (MICAL), mRNA |
| 0 |
0 |
5 |
2 |
9 |
NM_176445.1 |
CG33208-RC, transcript variant C (MICAL), mRNA |
| 0 |
0 |
5 |
2 |
9 |
NM_176449.1 |
CG33208-RH, transcript variant H (MICAL), mRNA |
| 0 |
0 |
5 |
2 |
9 |
NM_176448.1 |
CG33208-RG, transcript variant G (MICAL), mRNA |
| 0 |
0 |
5 |
2 |
9 |
NM_176446.1 |
CG33208-RD, transcript variant D (MICAL), mRNA |
| 0 |
0 |
5 |
2 |
8 |
NM_176443.1 |
CG33208-RE, transcript variant E (MICAL), mRNA |
| 0 |
0 |
5 |
2 |
8 |
NM_176444.1 |
CG33208-RB, transcript variant B (MICAL), mRNA |
| 0 |
0 |
4 |
17 |
15 |
NM_168387.1 |
CG6711-RA (Taf2), mRNA |
| 0 |
0 |
4 |
11 |
15 |
NM_168640.1 |
CG5942-RD, transcript variant D (brm), mRNA |
| 0 |
0 |
4 |
11 |
15 |
NM_080497.4 |
CG5942-RC, transcript variant C (brm), mRNA |
| 0 |
0 |
4 |
11 |
15 |
NM_168641.1 |
CG5942-RB, transcript variant B (brm), mRNA |
| 0 |
0 |
4 |
11 |
15 |
NM_080498.2 |
CG5942-RA, transcript variant A (brm), mRNA |
| 0 |
0 |
3 |
13 |
31 |
NM_133114.2 |
CG32541-RA (CG32541), mRNA |
| 0 |
0 |
3 |
8 |
32 |
NM_167685.1 |
CG12701-RB, transcript variant B (CG12701), mRNA |
| 0 |
0 |
3 |
8 |
32 |
NM_134512.4 |
CG12701-RA, transcript variant A (CG12701), mRNA |
| 0 |
0 |
3 |
8 |
28 |
NM_167620.2 |
CG32547-RA (CG32547), mRNA |
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