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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8580R-3 
 Symbol bhr  Full Name bhringi 
 CG No CG8580  Old CG No CG8580 
 Synonyms EP(3)0906, CG8580, anon-WO0118547.397, bhr 
 Accession No (Link to NCBI) NM_139856.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGCCTGTGCAACCCTGAAA 
 Primer Seq. 3' 
aatctagaggtaccTCGTAGCGCTCCCTTAGCTGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGGCCCTGT GCAACCCTGA AACGAGCCCT AGACTGGGAG TCGATGAACC AAGCGGCCTC 
0061 CGAAGCGCCG GCGTATGCAA TCCCTTTGGC CAGGCTGGGA GCAATGCAGG TCCAGCGTCG 
0121 CCATCCCGCG ACGGTCCCAG CACCTCGGCT GGTCTGCCCC ACACGCCCAG CAACCGATTC 
0181 GCCAAGGATA GCACCGAACC TAGTCCGTTC AGTGAGTCGT CGCTGGCCAA AATGTCACCA 
0241 GACAAAATGG CCGAGAGCTT GTGCAATGAG ATCAAGAGAC TGCACAAGCG CCAAACAGCT 
0301 GCCGATCACT TCGTCGGCCT TGGAACGCAT GCAGGATTCG GAGTCCAGCG GATCGGAGAT 
0361 GGGTCCAGAG AGTCCGCGCC GCCCGGACAG TCCACAGAAC CTGATGCGCC ACGGCGAAAA 
0421 GGCCCTGTTC ACGGTTCAAG CAGGTGCAGC TCATTTGCGA GAGCATGATC AAGGAGCGCG 
0481 AGAATCAGCT AAGGGAGCGC TACGA 
 in silico PCR Fragment 
0001 ATGG-CCTGT GCAACCCTGA AACGAGCCCT AGACTGGGAG TCGATGAACC -AGCGGCCTC 
0061 CGAAGCGCCG GCGT-TGCAA TCCCTTTGGC CAGGCTGGGA GCAATGCAGG TCCAGCGTCG 
0121 CCATCCCGCG ACGGTCCCAG CACCTCGGCT GGTCTGCCCC ACACGCCCAG CAACCGATTC 
0181 GCCAAGGATA GCACCGAACC TAGTCCGTTC AGTGAGTCGT CGCTGGCCAA AATGTCACCA 
0241 GACAAAATGG CCGAGAGCTT GTGCAATGAG ATCAAGAGAC TGCACAAGCG -CAAACAGCT 
0301 GCCGATCACT TCGTCGGCCT TGGAACGCAT GCAGGATTCG GAGTCCAGCG GATCGGAGAT 
0361 GGGTCCAGAG AGTCCGCGCC GCCCGGACAG TCCACAGAAC CTGATGCGCC ACGGCGAAAA 
0421 GGCCCTGTTC AC-GTTCAAG CAGGTGCAGC TCATTTGCGA GAGCATGATC AAGGAGCGCG 
0481 AGAATCAGCT AAGGGAGCGC TACGA 
 Assemble Data 

8580R-3.IR_full       1   ATGGCCCTGTGCAACCCTGAAACGAGCCCTAGACTGGGAGTCGATGAACCAAGCGGCCTC 60
                          |||| ||||||||||||||||||||||||||||||||||||||||||||| |||||||||
8580R-3.in silico     1   ATGG-CCTGTGCAACCCTGAAACGAGCCCTAGACTGGGAGTCGATGAACC-AGCGGCCTC 60


8580R-3.IR_full       61  CGAAGCGCCGGCGTATGCAATCCCTTTGGCCAGGCTGGGAGCAATGCAGGTCCAGCGTCG 120
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
8580R-3.in silico     61  CGAAGCGCCGGCGT-TGCAATCCCTTTGGCCAGGCTGGGAGCAATGCAGGTCCAGCGTCG 120


8580R-3.IR_full       121 CCATCCCGCGACGGTCCCAGCACCTCGGCTGGTCTGCCCCACACGCCCAGCAACCGATTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8580R-3.in silico     121 CCATCCCGCGACGGTCCCAGCACCTCGGCTGGTCTGCCCCACACGCCCAGCAACCGATTC 180


8580R-3.IR_full       181 GCCAAGGATAGCACCGAACCTAGTCCGTTCAGTGAGTCGTCGCTGGCCAAAATGTCACCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8580R-3.in silico     181 GCCAAGGATAGCACCGAACCTAGTCCGTTCAGTGAGTCGTCGCTGGCCAAAATGTCACCA 240


8580R-3.IR_full       241 GACAAAATGGCCGAGAGCTTGTGCAATGAGATCAAGAGACTGCACAAGCGCCAAACAGCT 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
8580R-3.in silico     241 GACAAAATGGCCGAGAGCTTGTGCAATGAGATCAAGAGACTGCACAAGCG-CAAACAGCT 300


8580R-3.IR_full       301 GCCGATCACTTCGTCGGCCTTGGAACGCATGCAGGATTCGGAGTCCAGCGGATCGGAGAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8580R-3.in silico     301 GCCGATCACTTCGTCGGCCTTGGAACGCATGCAGGATTCGGAGTCCAGCGGATCGGAGAT 360


8580R-3.IR_full       361 GGGTCCAGAGAGTCCGCGCCGCCCGGACAGTCCACAGAACCTGATGCGCCACGGCGAAAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8580R-3.in silico     361 GGGTCCAGAGAGTCCGCGCCGCCCGGACAGTCCACAGAACCTGATGCGCCACGGCGAAAA 420


8580R-3.IR_full       421 GGCCCTGTTCACGGTTCAAGCAGGTGCAGCTCATTTGCGAGAGCATGATCAAGGAGCGCG 480
                          |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
8580R-3.in silico     421 GGCCCTGTTCAC-GTTCAAGCAGGTGCAGCTCATTTGCGAGAGCATGATCAAGGAGCGCG 480


8580R-3.IR_full       481 AGAATCAGCTAAGGGAGCGCTACGA 505
                          |||||||||||||||||||||||||
8580R-3.in silico     481 AGAATCAGCTAAGGGAGCGCTACGA 505



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_139856.2  CG8580-RA, transcript variant A (bhr), mRNA 
100   482  0  0  0  NM_168211.1  CG8580-RB, transcript variant B (bhr), mRNA 
0.2   1  2  2  4  NM_140968.2  CG11796-RB, transcript variant B (CG11796), mRNA 
0.2   1  2  2  4  NM_168857.1  CG11796-RA, transcript variant A (CG11796), mRNA 
0   0  2  4  2  NM_136013.1  CG15148-RA, transcript variant A (btv), mRNA 
0   0  2  4  2  NM_001042904.1  CG15148-RB, transcript variant B (btv), mRNA 
0   0  2  4  2  NM_001042905.1  CG15148-RC, transcript variant C (btv), mRNA 
0   0  2  3  4  NM_130679.2  CG3526-RB, transcript variant B (CG3526), mRNA 
0   0  2  3  4  NM_166954.1  CG3526-RA, transcript variant A (CG3526), mRNA 
0   0  2  3  3  NM_135522.2  CG4957-RA (CG4957), mRNA 
0   0  2  2  5  NM_057981.3  CG4063-RA (ebi), mRNA 
0   0  2  2  5  NM_169700.2  CG31045-RA, transcript variant A (Mhcl), mRNA 
0   0  2  2  5  NM_001043250.1  CG31045-RG, transcript variant G (Mhcl), mRNA 
0   0  2  2  5  NM_001043251.1  CG31045-RF, transcript variant F (Mhcl), mRNA 
0   0  2  2  5  NM_169701.2  CG31045-RB, transcript variant B (Mhcl), mRNA 
0   0  2  2  2  NM_134935.2  CG3248-RA (CG3248), mRNA 
0   0  2  2  2  NM_166790.2  CG32019-RA, transcript variant A (bt), mRNA 
0   0  2  2  2  NM_205875.1  CG32019-RE, transcript variant E (bt), mRNA 
0   0  2  2  2  NM_205876.1  CG32019-RC, transcript variant C (bt), mRNA 
0   0  2  2  2  NM_205877.1  CG32019-RD, transcript variant D (bt), mRNA 
0   0  1  6  3  NM_176734.1  CG33206-RA, transcript variant A (l(1)G0168), mRNA 
0   0  1  6  3  NM_176735.1  CG33206-RB, transcript variant B (l(1)G0168), mRNA 
0   0  1  4  14  NM_135239.1  CG18304-RA (CG18304), mRNA 
0   0  1  4  2  NM_079716.2  CG6705-RA, transcript variant A (tsl), mRNA 
0   0  1  4  2  NM_169969.1  CG6705-RB, transcript variant B (tsl), mRNA 
0   0  1  3  4  NM_057605.3  CG17907-RA (Ace), mRNA 
0   0  1  2  9  NM_134978.2  CG16857-RA (CG16857), mRNA 
0   0  1  2  2  NM_176251.1  CG33151-RA (Gr59e), mRNA 
0   0  0  79  325  NM_167482.3  CG32580-RA (CG32580), mRNA 
0   0  0  6  7  NM_168422.1  CG7958-RB, transcript variant B (tna), mRNA 

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